Kaelyn Sumigray, PhD
Assistant Professor of Genetics; Co-Director, Yale Summer Enrichment Research Experience, Yale Center for Clinical Investigation (YCCI)
Research & Publications
Biography
News
Locations
Research Summary
Our goal as a lab is to understand the factors that affect stem cell niche morphogenesis and function. To this end, we study the formation of the mammalian intestinal stem cell niche, the crypt. Through confocal microscopy, transcriptome analysis, and 3D organoid culture, we are uncovering novel contributors to crypt morphogenesis and the impacts they have on tissue function.
Current projects include regulation of cell-extracellular matrix attachments, changes in the extracellular matrix during crypt formation, roles for planar cell polarity in crypt morphogenesis, as well as recruitment and involvement of other cell types that instruct crypt morphogenesis. Details can be found on the lab website.
Coauthors
Research Interests
Cell Adhesion; Cytoskeleton; Intestinal Polyps; Intestine, Small; Actin Cytoskeleton; Morphogenesis; Stem Cells; Cell Polarity; Organogenesis; Cell Shape; Stem Cell Niche
Selected Publications
- Generation and Manipulation of Rat Intestinal Organoids.Zagoren E, Santos A, Ameen N, Sumigray K. Generation and Manipulation of Rat Intestinal Organoids. Journal Of Visualized Experiments 2023 PMID: 37427951, DOI: 10.3791/65343.
- Antibiotic-induced microbial depletion enhances murine small intestinal epithelial growth in a serotonin-dependent mannerSalvi P, Shaughnessy M, Sumigray K, Cowles R. Antibiotic-induced microbial depletion enhances murine small intestinal epithelial growth in a serotonin-dependent manner. AJP Gastrointestinal And Liver Physiology 2023, 325: g80-g91. PMID: 37158470, DOI: 10.1152/ajpgi.00113.2022.
- Sa1225 BIFUNCTIONALITY OF UNCONVENTIONAL MYOSIN 5B (MYO5B) IN INTESTINAL PHYSIOLOGY: ROLE IN MVID AND GENERATION OF CFTR HIGH EXPRESSER (CHE) CELLS IN MICE.Santos A, dos Reis D, Sumigray K, Ameen N. Sa1225 BIFUNCTIONALITY OF UNCONVENTIONAL MYOSIN 5B (MYO5B) IN INTESTINAL PHYSIOLOGY: ROLE IN MVID AND GENERATION OF CFTR HIGH EXPRESSER (CHE) CELLS IN MICE. Gastroenterology 2023, 164: s-331. DOI: 10.1016/s0016-5085(23)01758-4.
- Vibrio cholerae biofilms use modular adhesins with glycan-targeting and nonspecific surface binding domains for colonizationHuang X, Nero T, Weerasekera R, Matej K, Hinbest A, Jiang Z, Lee R, Wu L, Chak C, Nijjer J, Gibaldi I, Yang H, Gamble N, Ng W, Malaker S, Sumigray K, Olson R, Yan J. Vibrio cholerae biofilms use modular adhesins with glycan-targeting and nonspecific surface binding domains for colonization. Nature Communications 2023, 14: 2104. PMID: 37055389, PMCID: PMC10102183, DOI: 10.1038/s41467-023-37660-0.
- CFTR High Expresser Cells in cystic fibrosis and intestinal diseasesdos Reis D, Dastoor P, Santos A, Sumigray K, Ameen N. CFTR High Expresser Cells in cystic fibrosis and intestinal diseases. Heliyon 2023, 9: e14568. PMID: 36967909, PMCID: PMC10031467, DOI: 10.1016/j.heliyon.2023.e14568.
- Maf family transcription factors are required for nutrient uptake in the mouse neonatal gutBara A, Chen L, Ma C, Underwood J, Moreci R, Sumigray K, Sun T, Diao Y, Verzi M, Lechler T. Maf family transcription factors are required for nutrient uptake in the mouse neonatal gut. Development 2022, 149 PMID: 36504079, PMCID: PMC10112929, DOI: 10.1242/dev.201251.
- Serotonin Activity Is Correlated with Intestinal Stem Cell Proliferation in Small Intestinal CryptsShah P, Sumigray K, Cowles R. Serotonin Activity Is Correlated with Intestinal Stem Cell Proliferation in Small Intestinal Crypts. Journal Of The American College Of Surgeons 2022, 235: s189-s190. DOI: 10.1097/01.xcs.0000894456.99853.6f.
- Loss of Serum Glucocorticoid-Inducible Kinase 1 SGK1 Worsens Malabsorption and Diarrhea in Microvillus Inclusion Disease (MVID)Ahsan K, dos Reis DC, Barbieri A, Sumigray KD, Nottoli T, Salas PJ, Ameen NA. Loss of Serum Glucocorticoid-Inducible Kinase 1 SGK1 Worsens Malabsorption and Diarrhea in Microvillus Inclusion Disease (MVID). Journal Of Clinical Medicine 2022, 11: 4179. PMID: 35887942, PMCID: PMC9319011, DOI: 10.3390/jcm11144179.
- Serum and Glucocorticoid‐Inducible Kinase 1 (SGK1): An Important Contributor to Diarrhea and Malabsorption in Microvillus Inclusion Disease (MVID)Ahsan K, Reis D, Barbieri A, Sumigray K, Nottoli T, Salas P, Ameen N. Serum and Glucocorticoid‐Inducible Kinase 1 (SGK1): An Important Contributor to Diarrhea and Malabsorption in Microvillus Inclusion Disease (MVID). The FASEB Journal 2022, 36 DOI: 10.1096/fasebj.2022.36.s1.r5730.
- Serum and Glucocorticoid-Inducible Kinase 1 (SGK1): An Important Contributor to Diarrhea and Malabsorption in Microvillus Inclusion Disease (MVID).Ahsan MK, Dos Reis DC, Barbieri A, Sumigray K, Nottoli T, Salas PJ, Ameen NA. Serum and Glucocorticoid-Inducible Kinase 1 (SGK1): An Important Contributor to Diarrhea and Malabsorption in Microvillus Inclusion Disease (MVID). FASEB Journal : Official Publication Of The Federation Of American Societies For Experimental Biology 2022, 36 Suppl 1 PMID: 35553440, DOI: 10.1096/fasebj.2022.36.S1.R5730.
- Lysosome-Rich Enterocytes Mediate Protein Absorption in the Vertebrate GutPark J, Levic DS, Sumigray KD, Bagwell J, Eroglu O, Block CL, Eroglu C, Barry R, Lickwar CR, Rawls JF, Watts SA, Lechler T, Bagnat M. Lysosome-Rich Enterocytes Mediate Protein Absorption in the Vertebrate Gut. Developmental Cell 2019, 51: 7-20.e6. PMID: 31474562, PMCID: PMC6783362, DOI: 10.1016/j.devcel.2019.08.001.
- Cellular Dynamics Driving Elongation of the GutSumigray K, Lechler T. Cellular Dynamics Driving Elongation of the Gut. Developmental Cell 2018, 46: 127-128. PMID: 30016614, DOI: 10.1016/j.devcel.2018.06.027.
- Morphogenesis and Compartmentalization of the Intestinal CryptSumigray KD, Terwilliger M, Lechler T. Morphogenesis and Compartmentalization of the Intestinal Crypt. Developmental Cell 2018, 45: 183-197.e5. PMID: 29689194, PMCID: PMC5987226, DOI: 10.1016/j.devcel.2018.03.024.
- Spine Patterning Is Guided by Segmentation of the Notochord SheathWopat S, Bagwell J, Sumigray KD, Dickson AL, Huitema LFA, Poss KD, Schulte-Merker S, Bagnat M. Spine Patterning Is Guided by Segmentation of the Notochord Sheath. Cell Reports 2018, 22: 2026-2038. PMID: 29466731, PMCID: PMC5860813, DOI: 10.1016/j.celrep.2018.01.084.
- Rap1 acts via multiple mechanisms to position Canoe and adherens junctions and mediate apical-basal polarity establishmentBonello TT, Perez-Vale KZ, Sumigray KD, Peifer M. Rap1 acts via multiple mechanisms to position Canoe and adherens junctions and mediate apical-basal polarity establishment. Development 2017, 145: dev157941. PMID: 29361565, PMCID: PMC5825837, DOI: 10.1242/dev.157941.
- Abelson kinase acts as a robust, multifunctional scaffold in regulating embryonic morphogenesisRogers EM, Spracklen AJ, Bilancia CG, Sumigray KD, Allred SC, Nowotarski SH, Schaefer KN, Ritchie BJ, Peifer M. Abelson kinase acts as a robust, multifunctional scaffold in regulating embryonic morphogenesis. Molecular Biology Of The Cell 2016, 27: 2613-2631. PMID: 27385341, PMCID: PMC4985262, DOI: 10.1091/mbc.e16-05-0292.
- The Arp2/3 complex has essential roles in vesicle trafficking and transcytosis in the mammalian small intestineZhou K, Sumigray KD, Lechler T. The Arp2/3 complex has essential roles in vesicle trafficking and transcytosis in the mammalian small intestine. Molecular Biology Of The Cell 2015, 26: 1995-2004. PMID: 25833710, PMCID: PMC4472011, DOI: 10.1091/mbc.e14-10-1481.
- Epigenetic control of intestinal barrier function and inflammation in zebrafishMarjoram L, Alvers A, Deerhake ME, Bagwell J, Mankiewicz J, Cocchiaro JL, Beerman RW, Willer J, Sumigray KD, Katsanis N, Tobin DM, Rawls JF, Goll MG, Bagnat M. Epigenetic control of intestinal barrier function and inflammation in zebrafish. Proceedings Of The National Academy Of Sciences Of The United States Of America 2015, 112: 2770-2775. PMID: 25730872, PMCID: PMC4352795, DOI: 10.1073/pnas.1424089112.
- Chapter Twelve Cell Adhesion in Epidermal Development and Barrier FormationSumigray KD, Lechler T. Chapter Twelve Cell Adhesion in Epidermal Development and Barrier Formation. 2015, 112: 383-414. PMID: 25733147, PMCID: PMC4737682, DOI: 10.1016/bs.ctdb.2014.11.027.
- Cell-Cell Adhesions and Cell Contractility Are Upregulated upon Desmosome DisruptionSumigray K, Zhou K, Lechler T. Cell-Cell Adhesions and Cell Contractility Are Upregulated upon Desmosome Disruption. PLOS ONE 2014, 9: e101824. PMID: 25006807, PMCID: PMC4090201, DOI: 10.1371/journal.pone.0101824.
- FRAP Analysis Reveals Stabilization of Adhesion Structures in the Epidermis Compared to Cultured KeratinocytesFoote HP, Sumigray KD, Lechler T. FRAP Analysis Reveals Stabilization of Adhesion Structures in the Epidermis Compared to Cultured Keratinocytes. PLOS ONE 2013, 8: e71491. PMID: 23977053, PMCID: PMC3747223, DOI: 10.1371/journal.pone.0071491.
- Rap1 and Canoe/afadin are essential for establishment of apical–basal polarity in the Drosophila embryoChoi W, Harris NJ, Sumigray KD, Peifer M. Rap1 and Canoe/afadin are essential for establishment of apical–basal polarity in the Drosophila embryo. Molecular Biology Of The Cell 2013, 24: 945-963. PMID: 23363604, PMCID: PMC3608504, DOI: 10.1091/mbc.e12-10-0736.
- Noncentrosomal microtubules and type II myosins potentiate epidermal cell adhesion and barrier formationSumigray KD, Foote HP, Lechler T. Noncentrosomal microtubules and type II myosins potentiate epidermal cell adhesion and barrier formation. Journal Of Cell Biology 2012, 199: 513-525. PMID: 23091070, PMCID: PMC3483132, DOI: 10.1083/jcb.201206143.
- Cell Shape by Coercion: Par1 and aPKC Put the Squeeze on JunctionsSumigray KD, Peifer M. Cell Shape by Coercion: Par1 and aPKC Put the Squeeze on Junctions. Developmental Cell 2012, 22: 907-908. PMID: 22595665, DOI: 10.1016/j.devcel.2012.04.016.
- Desmoplakin controls microvilli length but not cell adhesion or keratin organization in the intestinal epitheliumSumigray KD, Lechler T. Desmoplakin controls microvilli length but not cell adhesion or keratin organization in the intestinal epithelium. Molecular Biology Of The Cell 2012, 23: 792-799. PMID: 22238362, PMCID: PMC3290639, DOI: 10.1091/mbc.e11-11-0923.
- Control of cortical microtubule organization and desmosome stability by centrosomal proteinsSumigray KD, Lechler T. Control of cortical microtubule organization and desmosome stability by centrosomal proteins. BioArchitecture 2011, 1: 221-224. PMID: 22754612, PMCID: PMC3384573, DOI: 10.4161/bioa.18403.
- Lis1 is essential for cortical microtubule organization and desmosome stability in the epidermisSumigray KD, Chen H, Lechler T. Lis1 is essential for cortical microtubule organization and desmosome stability in the epidermis. Journal Of Cell Biology 2011, 194: 631-642. PMID: 21844209, PMCID: PMC3160577, DOI: 10.1083/jcb.201104009.
- Dissecting cell adhesion cross-talk with micropatternsSumigray KD, Lechler T. Dissecting cell adhesion cross-talk with micropatterns. Proceedings Of The National Academy Of Sciences Of The United States Of America 2010, 107: 13199-13200. PMID: 20639470, PMCID: PMC2922164, DOI: 10.1073/pnas.1008253107.