Featured Publications
The indelible toll of enteric pathogens: Prevalence, clinical characterization, and seasonal trends in patients with acute community-acquired diarrhea in disenfranchised communities
Osman M, Kassem I, Dabboussi F, Cummings K, Hamze M. The indelible toll of enteric pathogens: Prevalence, clinical characterization, and seasonal trends in patients with acute community-acquired diarrhea in disenfranchised communities. PLOS ONE 2023, 18: e0282844. PMID: 36913372, PMCID: PMC10010529, DOI: 10.1371/journal.pone.0282844.Peer-Reviewed Original ResearchConceptsEnteropathogenic E. coliEnteroaggregative Escherichia coliEnterotoxigenic E. coliEnteric pathogensCross-sectional community-based studyAcute community-acquired diarrheaMultivariable logistic regression modelCommon parasitic agentsCommunity-acquired diarrheaCommunity-based studyPublic health challengeLogistic regression modelsGII infectionsAcute diarrheaMiddle-income countriesGastrointestinal PanelDiarrheic patientsEnteric infectionsOverall prevalenceRisk factorsStool samplesClinical characterizationETEC infectionDiarrheal diseaseEtiologic agentEvidence for the transmission of antimicrobial resistant bacteria between humans and companion animals: A scoping review
Jin M, Osman M, Green B, Yang Y, Ahuja A, Lu Z, Cazer C. Evidence for the transmission of antimicrobial resistant bacteria between humans and companion animals: A scoping review. One Health 2023, 17: 100593. PMID: 37448771, PMCID: PMC10336692, DOI: 10.1016/j.onehlt.2023.100593.Peer-Reviewed Original ResearchRefugee settlements, sewage pollution, COVID-19 and the unfolding cholera outbreak in Lebanon
Kassem I, Osman M, Jaafar H, Omari K. Refugee settlements, sewage pollution, COVID-19 and the unfolding cholera outbreak in Lebanon. Journal Of Travel Medicine 2022, 29: taac142. PMID: 36434781, DOI: 10.1093/jtm/taac142.Peer-Reviewed Original ResearchCatch-22: War, Refugees, COVID-19, and the Scourge of Antimicrobial Resistance
Osman M, Cummings K, Omari K, Kassem I. Catch-22: War, Refugees, COVID-19, and the Scourge of Antimicrobial Resistance. Frontiers In Medicine 2022, 9: 921921. PMID: 35814789, PMCID: PMC9263824, DOI: 10.3389/fmed.2022.921921.Peer-Reviewed Original ResearchIntegrated Surveillance System for Controlling COVID-19 on a University Campus, 2020‒2021
Meredith G, Osman M, Cazer C, Cummings K, Hecht J, Madsen C, Santacrose L, Dubovi A, Clarkberg M, Johnson T, Fitzpatrick M, Parrilla L, Li Y, Francis L, Weisfuse I, Travis A, Jones A, Warnick L, Koretzky G. Integrated Surveillance System for Controlling COVID-19 on a University Campus, 2020‒2021. American Journal Of Public Health 2022, 112: 980-984. PMID: 35728024, PMCID: PMC9222446, DOI: 10.2105/ajph.2022.306838.Peer-Reviewed Original ResearchMachine-learning approaches prevent post-treatment resistance-gaining bacterial recurrences
Osman M, Mahieu R, Eveillard M. Machine-learning approaches prevent post-treatment resistance-gaining bacterial recurrences. Trends In Microbiology 2022, 30: 612-614. PMID: 35599112, DOI: 10.1016/j.tim.2022.05.006.Peer-Reviewed Original ResearchA brewing storm: the impact of economic collapse on the access to antimicrobials in Lebanon
Kassem I, Osman M. A brewing storm: the impact of economic collapse on the access to antimicrobials in Lebanon. Journal Of Global Antimicrobial Resistance 2022, 29: 313-315. PMID: 35513228, DOI: 10.1016/j.jgar.2022.04.023.Peer-Reviewed Original ResearchAntimicrobial resistance in the protracted Syrian conflict: halting a war in the war
Osman M, Rafei R, Ismail M, Al Omari S, Mallat H, Dabboussi F, Cazer C, Karah N, Abbara A, Hamze M. Antimicrobial resistance in the protracted Syrian conflict: halting a war in the war. Future Microbiology 2021, 16: 825-845. PMID: 34223789, DOI: 10.2217/fmb-2021-0040.Peer-Reviewed Original ResearchConceptsSyrian crisisSyrian conflictMassive displacementHost countryEffective policiesMiddle EastInternational health organizationsWarExhausting conditionsConflictCrisisCurrent situationCurrent obstaclesKey interventionsRefugeesGovernmentPolicySyriaCountriesEuropeContextOrganizationEastThreatEmergenceDrug-Resistant Tuberculosis, Lebanon, 2016 – 2017 - Volume 25, Number 3—March 2019 - Emerging Infectious Diseases journal - CDC
Achkar S, Demanche C, Osman M, Rafei R, Ismail M, Yaacoub H, Pinçon C, Duthoy S, De Matos F, Gaudin C, Trovato A, Cirillo D, Hamze M, Supply P. Drug-Resistant Tuberculosis, Lebanon, 2016 – 2017 - Volume 25, Number 3—March 2019 - Emerging Infectious Diseases journal - CDC. Emerging Infectious Diseases 2019, 25: 564-568. PMID: 30789124, PMCID: PMC6390733, DOI: 10.3201/eid2503.181375.Peer-Reviewed Original Research
2024
Genomic characterization of plasmid-borne colistin resistance variants, mcr-1.1 and mcr-1.26, in multidrug-resistant Escherichia coli isolated from backyard farm animals
Daaboul D, Kassem I, Omari K, Eltai N, Hassan J, Jamal H, Fayad S, Salma R, Tajani A, Bisha B, Hamze M, Oueslati S, Cummings K, Daboussi F, Naas T, Osman M. Genomic characterization of plasmid-borne colistin resistance variants, mcr-1.1 and mcr-1.26, in multidrug-resistant Escherichia coli isolated from backyard farm animals. Journal Of Global Antimicrobial Resistance 2024 PMID: 38969269, DOI: 10.1016/j.jgar.2024.06.009.Peer-Reviewed Original ResearchSyndromic Surveillance Tracks COVID-19 Cases in University and County Settings: Retrospective Observational Study
Wass L, Hoare D, Smits G, Osman M, Zhang N, Klepack W, Parrilla L, Busche J, Clarkberg M, Basu S, Cazer C. Syndromic Surveillance Tracks COVID-19 Cases in University and County Settings: Retrospective Observational Study. JMIR Public Health And Surveillance 2024, 10: e54551. PMID: 38952000, PMCID: PMC11220726, DOI: 10.2196/54551.Peer-Reviewed Original ResearchConceptsCOVID-19-like illnessSyndromic surveillanceCounty Health DepartmentSARS-CoV-2 test positivityCounty settingsMiddle-income countriesLow-resource settingsCounty levelReport surveillance dataHealth departmentsTest positivity rateLinear regression modelsPositive test rateTesting ratesCOVID-19 casesSelf-reportSymptom reportingCOVID-19 riskCOVID-19 testingCOVID-19Negative binomial modelRetrospective observational studyTest positivityCOVID-19 surveillanceObservational studyNeonatal sepsis due to NDM-1 and VIM-2 co-producing Pseudomonas aeruginosa in Morocco
Daaboul D, Osman M, Kassem I, Yassine I, Girlich D, Proust A, Mounir C, Zerouali K, Raymond J, Naas T, Oueslati S. Neonatal sepsis due to NDM-1 and VIM-2 co-producing Pseudomonas aeruginosa in Morocco. Journal Of Antimicrobial Chemotherapy 2024, 79: 1614-1618. PMID: 38804143, DOI: 10.1093/jac/dkae153.Peer-Reviewed Original ResearchNeonatal intensive care unitBlaVIM-2BlaNDM-1Intensive care unitCenter of Genomic EpidemiologyBlaVIM-2 geneIntegrative conjugative elementsWhole-genome sequencingAntimicrobial resistance genesBlaNDM-1 geneAntimicrobial susceptibility testingCarbapenem-resistant Pseudomonas aeruginosaCare unitLast-resort antibioticsSusceptible to colistinResistance to cefiderocolXDR P. aeruginosaGram-negative bacteriaCarba NPMetallo-b-lactamasesReducing treatment optionsConjugative elementsSequence typingESBL genesMinION technologyEvaluation of SARS-CoV-2 anti-Spike antibody levels and breakthrough infection risk among vaccinated adults in North Lebanon
Nour D, Ismail M, Osman M, Rafei R, Kasir D, Dabboussi F, Colson P, Hamze M. Evaluation of SARS-CoV-2 anti-Spike antibody levels and breakthrough infection risk among vaccinated adults in North Lebanon. PLOS ONE 2024, 19: e0302579. PMID: 38722969, PMCID: PMC11081361, DOI: 10.1371/journal.pone.0302579.Peer-Reviewed Original ResearchConceptsImmune responseAntibody titersAntibody levelsAnti-spike antibody levelsCharacteristics of immune responsesWaning immune responsesMultivariate logistic regression modelSARS-CoV-2 antibody titersLevel of antibody titerVaccinated patientsBNT162b2 vaccineUnivariate analysisLogistic regression modelsSputnik V vaccineBlood specimensPfizer-BioNTechVaccine doseImmune statusVaccinated adultsSARS-CoV-2InfectionNorth LebanonVaccineAstraZeneca vaccineTitersDraft genome sequences of antibiotic-resistant Serratia and Enterobacter species isolated from imported fresh produce in Georgia, USA
Kassem I, Wang J, Tajani A, Esseili M, Hassan J, Yassine I, Osman M, Bisha B. Draft genome sequences of antibiotic-resistant Serratia and Enterobacter species isolated from imported fresh produce in Georgia, USA. Microbiology Resource Announcements 2024, 13: e01139-23. PMID: 38695584, PMCID: PMC11237534, DOI: 10.1128/mra.01139-23.Peer-Reviewed Original ResearchEmergence of NDM-1, VIM-4 and CTX-M-15-co-harbouring Enterobacter xiangfangensis in a bloodstream infection
Daaboul D, Kassem I, Yassine I, Hamze M, Dabboussi F, Girlich D, Oueslati S, Naas T, Osman M. Emergence of NDM-1, VIM-4 and CTX-M-15-co-harbouring Enterobacter xiangfangensis in a bloodstream infection. Journal Of Antimicrobial Chemotherapy 2024, 79: 923-925. PMID: 38297956, DOI: 10.1093/jac/dkae008.Peer-Reviewed Original Research
2023
Multidrug-resistant pathogens contaminate river water used in irrigation in disenfranchised communities
Osman M, Daaboul D, Tajani A, El Omari K, Bisha B, Hassan J, Cazer C, Fiorella K, Karah N, Abbara A, Hamze M, Cummings K, Naas T, Kassem I. Multidrug-resistant pathogens contaminate river water used in irrigation in disenfranchised communities. Journal Of Global Antimicrobial Resistance 2023, 36: 175-180. PMID: 38154747, DOI: 10.1016/j.jgar.2023.12.016.Peer-Reviewed Original ResearchThe occurrence of the carbapenemase gene, bla NDM-5, on a transmissible IncX3 plasmid in multidrug-resistant Escherichia coli isolated from a farm dog
Daaboul D, Kassem I, El Omari K, Dabboussi F, Oueslati S, Naas T, Osman M. The occurrence of the carbapenemase gene, bla NDM-5, on a transmissible IncX3 plasmid in multidrug-resistant Escherichia coli isolated from a farm dog. Journal Of Global Antimicrobial Resistance 2023, 36: 59-61. PMID: 38128725, DOI: 10.1016/j.jgar.2023.12.006.Peer-Reviewed Original ResearchGenomic analysis of Shigella isolates from Lebanon reveals marked genetic diversity and antimicrobial resistance
Yassine I, Rafei R, de la Gandara M, Osman M, Fabre L, Dabboussi F, Hamze M, Weill F. Genomic analysis of Shigella isolates from Lebanon reveals marked genetic diversity and antimicrobial resistance. Microbial Genomics 2023, 9: 001157. PMID: 38100171, PMCID: PMC10763507, DOI: 10.1099/mgen.0.001157.Peer-Reviewed Original ResearchConceptsExtended-spectrum beta-lactamase genesThird-generation cephalosporinsMultidrug resistance phenotypeBeta-lactamase genesAntimicrobial resistance determinantsPrevalent serogroupsClinical isolatesQuinolone resistanceDNA gyrase genesAntimicrobial resistanceResistance determinantsCTXResistance phenotypeAmino acid substitutionsGyrase genesIsolatesNorth LebanonFirst studyAcid substitutionsPoint mutationsGenomic analysisCephalosporinsEmergence of Extended-Spectrum Cephalosporin- and Colistin-Resistant Enterobacterales in Otherwise Healthy University Students
Osman M, Yassine I, Hamze M, Al Mir H, Tajani A, Bisha B, Cummings K, Madec J, Haenni M, Kassem I. Emergence of Extended-Spectrum Cephalosporin- and Colistin-Resistant Enterobacterales in Otherwise Healthy University Students. Microbial Drug Resistance 2023, 30: 101-107. PMID: 38011748, DOI: 10.1089/mdr.2023.0213.Peer-Reviewed Original ResearchLast-resort antibioticsHealthy university studentsExtended-spectrum cephalosporin-resistant isolatesComprehensive antimicrobial stewardship programAntimicrobial stewardship programsColistin-resistant isolatesExtended-spectrum cephalosporinsCephalosporin-resistant isolatesColistin-resistant EnterobacteralesMultidrug-resistant bacteriaMiddle-income countriesEvidence-based practiceAntimicrobial stewardshipStewardship programsOccurrence of resistanceCommunity settingsAntimicrobial resistanceClinical samplesAntibiotic resistanceFecal samplesResistance determinantsAntibioticsPublic healthMultiple factorsIsolatesThe emergence of carbapenemase-producing Enterobacterales in hospitals: a major challenge for a debilitated healthcare system in Lebanon
Daaboul D, Oueslati S, Rima M, Kassem I, Mallat H, Birer A, Girlich D, Hamze M, Dabboussi F, Osman M, Naas T. The emergence of carbapenemase-producing Enterobacterales in hospitals: a major challenge for a debilitated healthcare system in Lebanon. Frontiers In Public Health 2023, 11: 1290912. PMID: 38074718, PMCID: PMC10699444, DOI: 10.3389/fpubh.2023.1290912.Peer-Reviewed Original ResearchConceptsKirby-Bauer disk diffusionDifferent Lebanese hospitalsSelf-medication practicesDisk diffusionPublic health systemCarbapenemase-producing EnterobacteralesConventional diagnostic methodsESBL producersHospital settingAntibiotic susceptibilityLebanese hospitalsClinical settingHospitalHealth systemSusceptibility profilesAntimicrobial resistanceHealthcare systemPlasmid typesHealthcare challengesDiagnostic methodsNDMCarbapenemsAMR awarenessIsolatesLebanese community