2009
A Modified ANK-1 Promoter Directs Uniform, Copy Number Dependent Gamma Globin Gene Expression at Therapeutic Levels of a in a Lentivirus Vector.
Harrow F, Cline A, Seidel N, Persons D, Gallagher P, Bodine D. A Modified ANK-1 Promoter Directs Uniform, Copy Number Dependent Gamma Globin Gene Expression at Therapeutic Levels of a in a Lentivirus Vector. Blood 2009, 114: 3565. DOI: 10.1182/blood.v114.22.3565.3565.Peer-Reviewed Original ResearchBeta-like globin genesMouse alpha-globinGamma-globin geneGamma-globin mRNAGlobin gene expressionLocus control regionGamma-globin expressionGlobin genesAlpha-globinHematopoietic stem cellsGene expressionEnhancer elementsMouse alpha-globin mRNAHuman gamma-globin geneBeta-globin locus control regionGamma-globin gene expressionSequence variantsTranscription initiation complexLCR elementsBeta-like globinCopy number-dependent expressionBinding of TFIIDAlpha-globin mRNACis-acting enhancer elementPromoter sequence variants
2007
Identification of a TFIID Binding Sequence That Increases Expression from the Ankyrin-1 Promoter: Application to Globin Gene Therapy
Laflamme K, Elnitski L, Wong C, Mary Y, Gallagher P, Bodine D. Identification of a TFIID Binding Sequence That Increases Expression from the Ankyrin-1 Promoter: Application to Globin Gene Therapy. Blood 2007, 110: 3741. DOI: 10.1182/blood.v110.11.3741.3741.Peer-Reviewed Original ResearchGene therapyEffective gene therapyGlobin gene therapyDifferent tissue-specific promotersTissue-specific promotersVector copiesViral vectorsDouble-copy vectorVirus vectorsHematopoietic stem cellsSpecific promotersPromoter libraryLibrary of promotersRecombinant virusesStem cellsInefficient productionRecent demonstrationReporter geneTG dinucleotideInsertional activationHigh titersΓ-globin expressionTherapeutic levelsVectorLocus control region
2006
Identification of a Novel Core Promoter Element That Enhances Transcription: Application to Ankyrin Promoters for Globin Gene Therapy.
Laflamme K, Elnitski L, Owen A, Gallagher P, Bodine D. Identification of a Novel Core Promoter Element That Enhances Transcription: Application to Ankyrin Promoters for Globin Gene Therapy. Blood 2006, 108: 3261. DOI: 10.1182/blood.v108.11.3261.3261.Peer-Reviewed Original ResearchGC-rich promoterLocus control regionΓ-globin expressionHematopoietic stem cellsTG dinucleotideErythroid cellsNovel core promoter elementΑ-globinDifferent tissue-specific promotersLike globin genesBinding regionsΓ-globin transcriptionANK-1 geneTranscription initiation complexΓ-globin geneErythroid K562 cellsConsensus promoter sequenceTranscriptional start siteCore promoter elementsRNA virus vectorMouse erythroid cellsK562 cellsStable transfection assaysWild-type promoterTissue-specific promoters