A Modified ANK-1 Promoter Directs Uniform, Copy Number Dependent Gamma Globin Gene Expression at Therapeutic Levels of a in a Lentivirus Vector.
Harrow F, Cline A, Seidel N, Persons D, Gallagher P, Bodine D. A Modified ANK-1 Promoter Directs Uniform, Copy Number Dependent Gamma Globin Gene Expression at Therapeutic Levels of a in a Lentivirus Vector. Blood 2009, 114: 3565. DOI: 10.1182/blood.v114.22.3565.3565.Peer-Reviewed Original ResearchBeta-like globin genesMouse alpha-globinGamma-globin geneGamma-globin mRNAGlobin gene expressionLocus control regionGamma-globin expressionGlobin genesAlpha-globinHematopoietic stem cellsGene expressionEnhancer elementsMouse alpha-globin mRNAHuman gamma-globin geneBeta-globin locus control regionGamma-globin gene expressionSequence variantsTranscription initiation complexLCR elementsBeta-like globinCopy number-dependent expressionBinding of TFIIDAlpha-globin mRNACis-acting enhancer elementPromoter sequence variantsGenome Expression Profiling of the First Hematopoietic Progenitors of the Mouse Embryo.
Baron M, Isern J, Fraser S, He Z, Tuck D, Schulz V, Gallagher P. Genome Expression Profiling of the First Hematopoietic Progenitors of the Mouse Embryo. Blood 2009, 114: 2536. DOI: 10.1182/blood.v114.22.2536.2536.Peer-Reviewed Original ResearchGenome expression profilingMouse embryosGFP reporterExpression profilingErythroid lineageLineage-specific cell typesEmbryonic globin genesDefinitive erythroid lineagesHematopoietic progenitorsGlobin gene expressionCandidate transcriptional regulatorsCell typesPrimitive erythroid lineageLineage-specific expressionEarly mouse embryosAnalysis of promotersEnd of gastrulationEmbryonic day 7.5Hematopoietic cell typesEarly hematopoietic progenitorsExpression of GATA2Distinct functional classesOrganelle biogenesisRibosome biogenesisPrimitive erythropoiesis