2020
Dolosigranulum pigrum Cooperation and Competition in Human Nasal Microbiota
Brugger SD, Eslami SM, Pettigrew MM, Escapa IF, Henke MT, Kong Y, Lemon KP. Dolosigranulum pigrum Cooperation and Competition in Human Nasal Microbiota. MSphere 2020, 5: e00852-20. PMID: 32907957, PMCID: PMC7485692, DOI: 10.1128/msphere.00852-20.Peer-Reviewed Original ResearchMeSH KeywordsBacteriaCarnobacteriaceaeChild, PreschoolGenomicsHumansInfantMicrobial InteractionsMicrobiotaNasopharynxPneumococcal InfectionsStaphylococcus aureusStreptococcus pneumoniaeConceptsMicrobe-microbe interactionsHuman nasal microbiotaGenomic analysisPhenotypic interactionsBiosynthetic gene clusterSmall genomesNasal microbiotaGene clusterAccessory genomeKey nutrientsNew insightsGenomic capacityBacterial residentsPathogenic speciesNasal microbiota compositionBeneficial bacteriumFunctional assaysDiverse repertoireHuman hostGenomeSpeciesAcid productionMicrobiotaNasal bacteriaBacteria
2017
Panel 6: Vaccines
Pettigrew MM, Alderson MR, Bakaletz LO, Barenkamp SJ, Hakansson AP, Mason KM, Nokso‐Koivisto J, Patel J, Pelton SI, Murphy TF. Panel 6: Vaccines. Otolaryngology 2017, 156: s76-s87. PMID: 28372533, PMCID: PMC5505493, DOI: 10.1177/0194599816632178.Peer-Reviewed Original ResearchConceptsPneumococcal otitis mediaOtitis mediaVaccine antigensVaccine developmentH influenzaePneumococcal serotypesHaemophilus influenzae protein DSignificant health care burdenAdditional pneumococcal serotypesCorrelates of protectionHealth care burdenRecurrent otitis mediaEffectiveness of vaccinesTympanostomy tube insertionProtein-based vaccinesCandidate vaccine antigensOM pathogensOM visitsPCV effectivenessPneumococcal vaccineRespiratory virusesVaccine protectionTube insertionMoraxella catarrhalisCare burden
2016
Density, Serotype Diversity, and Fitness of Streptococcus pneumoniae in Upper Respiratory Tract Cocolonization With Nontypeable Haemophilus influenzae
Lewnard JA, Huppert A, Givon-Lavi N, Pettigrew MM, Regev-Yochay G, Dagan R, Weinberger DM. Density, Serotype Diversity, and Fitness of Streptococcus pneumoniae in Upper Respiratory Tract Cocolonization With Nontypeable Haemophilus influenzae. The Journal Of Infectious Diseases 2016, 214: 1411-1420. PMID: 27540112, PMCID: PMC5079371, DOI: 10.1093/infdis/jiw381.Peer-Reviewed Original ResearchConceptsNontypeable Haemophilus influenzaeNTHi colonizationPneumococcal serotypesChildren 2Haemophilus influenzaeStreptococcus pneumoniaeUpper respiratory tract carriageSerotype diversityComplex otitis mediaNasopharyngeal pneumococcal carriagePneumococcal conjugate vaccineImmune-evasive phenotypePneumococcal serotype diversityNTHi carriageConjugate vaccinePneumococcal carriageOtitis mediaStudy visitNeutrophil resistanceCapsular thicknessLogistic regressionDensity associationsCarriageSerotypesColonization density
2015
Epidemiological Markers for Interactions Among Streptococcus pneumoniae, Haemophilus influenzae, and Staphylococcus aureus in Upper Respiratory Tract Carriage
Lewnard JA, Givon-Lavi N, Huppert A, Pettigrew MM, Regev-Yochay G, Dagan R, Weinberger DM. Epidemiological Markers for Interactions Among Streptococcus pneumoniae, Haemophilus influenzae, and Staphylococcus aureus in Upper Respiratory Tract Carriage. The Journal Of Infectious Diseases 2015, 213: 1596-1605. PMID: 26704617, PMCID: PMC4837906, DOI: 10.1093/infdis/jiv761.Peer-Reviewed Original ResearchMeSH KeywordsCarrier StateChild, PreschoolFemaleHaemophilus InfectionsHaemophilus influenzaeHeptavalent Pneumococcal Conjugate VaccineHumansInfantIsraelLongitudinal StudiesMalePneumococcal InfectionsRespiratory Tract InfectionsRisk FactorsStaphylococcal InfectionsStaphylococcus aureusStreptococcus pneumoniaeVaccines, ConjugateConceptsPneumococcal serotype replacementH. influenzaeSerotype replacementS. pneumoniaeHaemophilus influenzaeStreptococcus pneumoniaePneumococcal conjugate vaccine trialUpper respiratory tract carriagePneumococcal vaccine introductionS. aureus carriageMonths of ageStaphylococcus aureusPCV7 recipientsAureus carriageVaccine introductionVaccine trialsCarriage patternsConfounding factorsClinical interventionsInfluenzaeEpidemiological markersBedouin childrenPneumoniaeCarriageS. aureusStreptococcus pneumoniae biofilm formation and dispersion during colonization and disease
Chao Y, Marks LR, Pettigrew MM, Hakansson AP. Streptococcus pneumoniae biofilm formation and dispersion during colonization and disease. Frontiers In Cellular And Infection Microbiology 2015, 4: 194. PMID: 25629011, PMCID: PMC4292784, DOI: 10.3389/fcimb.2014.00194.Peer-Reviewed Original ResearchConceptsNasopharyngeal environmentConcomitant virus infectionPneumococcal biofilmsMiddle ear infectionRespiratory epithelial cellsMillions of infectionsPneumococcal biofilm formationPneumococcal diseaseEar infectionsSterile sitesInvasive diseasePneumococcal colonizationHigh prevalenceVirus infectionDisease processDistinct phenotypic propertiesMucosal surfacesStreptococcus pneumoniaeVaccine escapeHuman nasopharynxDiseaseEpithelial cellsInfectionPneumococciAntibiotic resistance
2014
Dynamic Changes in the Streptococcus pneumoniae Transcriptome during Transition from Biofilm Formation to Invasive Disease upon Influenza A Virus Infection
Pettigrew MM, Marks LR, Kong Y, Gent JF, Roche-Hakansson H, Hakansson AP. Dynamic Changes in the Streptococcus pneumoniae Transcriptome during Transition from Biofilm Formation to Invasive Disease upon Influenza A Virus Infection. Infection And Immunity 2014, 82: 4607-4619. PMID: 25135685, PMCID: PMC4249342, DOI: 10.1128/iai.02225-14.Peer-Reviewed Original ResearchConceptsWhole transcriptome shotgun sequencingPneumococcal transcriptomeTransparent colony phenotypeVirus infectionTranscriptional differencesShotgun sequencingUpregulated genesFebrile range temperaturesColony phenotypeInfluenza A Virus InfectionNutrient availabilityTranscriptomeS. pneumoniae pathogenesisA virus infectionProduction of bacteriocinsHost immune responsePlanktonic cellsBiofilm formationGenesPlanktonic bacteriaBiofilm bacteriaExtracellular ATPCarbohydrate metabolismBacteriaEnzymatic assay
2013
Panel 6: Vaccines
Pelton SI, Pettigrew MM, Barenkamp SJ, Godfroid F, Grijalva CG, Leach A, Patel J, Murphy TF, Selak S, Bakaletz LO. Panel 6: Vaccines. Otolaryngology 2013, 148: e90-e101. PMID: 23536534, PMCID: PMC4029613, DOI: 10.1177/0194599812466535.Peer-Reviewed Original ResearchConceptsAcute otitis mediaPneumococcal conjugate vaccineOtitis mediaDevelopment of correlatesCandidate antigensAnimal modelsClinical efficacy trialsEffectiveness of vaccinesBurden of diseaseNontypeable Haemophilus influenzaeHuman clinical trialsOtitis pathogensConjugate vaccineVaccine strategiesPneumococcal serotypesClinical trialsMoraxella catarrhalisAdditional serotypesNew adjuvantsEfficacy trialsObservational studyFrequent recurrenceChild healthVaccine antigensMore antigens
2012
Bacterial and viral interactions within the nasopharynx contribute to the risk of acute otitis media
Ruohola A, Pettigrew MM, Lindholm L, Jalava J, Räisänen KS, Vainionpää R, Waris M, Tähtinen PA, Laine MK, Lahti E, Ruuskanen O, Huovinen P. Bacterial and viral interactions within the nasopharynx contribute to the risk of acute otitis media. Journal Of Infection 2012, 66: 247-254. PMID: 23266462, PMCID: PMC3571106, DOI: 10.1016/j.jinf.2012.12.002.Peer-Reviewed Original ResearchConceptsRisk of AOMAcute otitis mediaMiddle ear effusionAOM groupRespiratory virusesAOM pathogensAcute symptomsOtitis mediaEar effusionM. catarrhalisAcute inflammatory signsNasopharyngeal bacterial colonizationRespiratory tract infectionsOtoscopic signsTract infectionsInflammatory signsNasopharyngeal samplesSymptomatic childrenNasopharyngeal bacteriaH. influenzaePneumatic otoscopyS. pneumoniaeViral interactionsBacterial colonizationEffusionVirulence of Streptococcus pneumoniae serotype 6C in experimental otitis media
Sabharwal V, Figueira M, Pelton SI, Pettigrew MM. Virulence of Streptococcus pneumoniae serotype 6C in experimental otitis media. Microbes And Infection 2012, 14: 712-718. PMID: 22414497, PMCID: PMC3382049, DOI: 10.1016/j.micinf.2012.02.008.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsChildChild, PreschoolChinchillaDisease Models, AnimalHumansNasopharynxOtitis MediaPneumococcal InfectionsSerotypingStreptococcus pneumoniaeVirulenceConceptsAcute otitis mediaExperimental otitis mediaPneumococcal conjugate vaccineOtitis mediaSerotype 6CInvasive pneumococcal diseaseOverall carriage rateLow-density infectionsMiddle ear infectionStreptococcus pneumoniae serotype 6CReplacement serotypesPneumococcal diseaseVaccine serotypesConjugate vaccineNonvaccine serotypesCarriage rateEar infectionsNasopharyngeal colonizationChinchilla modelStreptococcus pneumoniaeHost defenseSerotypesDisease potentialInfectionDisease
2011
Viral-Bacterial Interactions and Risk of Acute Otitis Media Complicating Upper Respiratory Tract Infection
Pettigrew MM, Gent JF, Pyles RB, Miller AL, Nokso-Koivisto J, Chonmaitree T. Viral-Bacterial Interactions and Risk of Acute Otitis Media Complicating Upper Respiratory Tract Infection. Journal Of Clinical Microbiology 2011, 49: 3750-3755. PMID: 21900518, PMCID: PMC3209086, DOI: 10.1128/jcm.01186-11.Peer-Reviewed Original ResearchMeSH KeywordsChild, PreschoolCohort StudiesComorbidityHaemophilus influenzaeHuman bocavirusHumansInfantMetapneumovirusMicrobial InteractionsMoraxella catarrhalisOtitis MediaPolymerase Chain ReactionProspective StudiesRespiratory Syncytial VirusesRespiratory Tract InfectionsRisk AssessmentStreptococcus pneumoniaeConceptsUpper respiratory tract infectionAcute otitis mediaRespiratory tract infectionsRespiratory syncytial virusOtitis mediaOtitis media riskTract infectionsViral loadSyncytial virusHaemophilus influenzaeStreptococcus pneumoniaeRespiratory Syncytial Virus LoadMedium-risk factorsRepeated-measures logistic regressionRSV viral loadViral-bacterial interactionsHigh viral loadSpecific virusesBacterial pathogensChildren ages 6Common complicationRespiratory virusesMedium riskProspective studyHuman bocavirusStreptococcus pneumoniae Serotype 6C: an Intra- and Interclonal Complex Comparison
Thomas JC, Kong Y, Sabharwal V, Pelton SI, Pettigrew MM. Streptococcus pneumoniae Serotype 6C: an Intra- and Interclonal Complex Comparison. Journal Of Bacteriology 2011, 193: 3409-3410. PMID: 21551297, PMCID: PMC3133277, DOI: 10.1128/jb.05079-11.Peer-Reviewed Original ResearchStreptococcus pneumoniae Clonal Complex 199: Genetic Diversity and Tissue-Specific Virulence
Thomas JC, Figueira M, Fennie KP, Laufer AS, Kong Y, Pichichero ME, Pelton SI, Pettigrew MM. Streptococcus pneumoniae Clonal Complex 199: Genetic Diversity and Tissue-Specific Virulence. PLOS ONE 2011, 6: e18649. PMID: 21533186, PMCID: PMC3077395, DOI: 10.1371/journal.pone.0018649.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsChinchillaDisease Models, AnimalGenes, BacterialGenetic VariationPneumococcal InfectionsStreptococcus pneumoniaeVirulenceConceptsChinchilla modelHeptavalent pneumococcal conjugate vaccinePneumococcal conjugate vaccineSerotype 19A isolatesMiddle ear isolatesS. pneumoniae isolatesLong-term effectivenessConjugate vaccinePneumococcal diseaseOtitis mediaVaccinated populationReplacement diseaseBlood isolatesInvasive diseaseCarriage isolatesPneumoniae isolatesCerebrospinal fluidEar isolatesFuture vaccinesImportant causeStreptococcus pneumoniaeMiddle earDiseaseVirulence potentialVaccineMicrobial Communities of the Upper Respiratory Tract and Otitis Media in Children
Laufer AS, Metlay JP, Gent JF, Fennie KP, Kong Y, Pettigrew MM. Microbial Communities of the Upper Respiratory Tract and Otitis Media in Children. MBio 2011, 2: 10.1128/mbio.00245-10. PMID: 21285435, PMCID: PMC3031303, DOI: 10.1128/mbio.00245-10.Peer-Reviewed Original ResearchMeSH KeywordsBacteriaChildChild, PreschoolCross-Sectional StudiesFemaleHumansInfantInfant, NewbornMaleMolecular Sequence DataNoseOtitis MediaPhylogenyStreptococcus pneumoniaeConceptsOtitis mediaUpper respiratory tractS. pneumoniaeRespiratory tractOtitis media treatmentRespiratory virus seasonS. pneumoniae colonizationOtitis media pathogensCross-sectional studyOtitis media susceptibilityPneumoniae colonizationDecreased riskPneumococcal colonizationNasal swabsFrequent causePrevention strategiesStreptococcus pneumoniaeDemographic dataProtective floraPneumoniaeSwabsChildrenCausal pathwaysTractRisk
2010
Capacity of serotype 19A and 15B/C Streptococcus pneumoniae isolates for experimental otitis media: Implications for the conjugate vaccine
Laufer AS, Thomas JC, Figueira M, Gent JF, Pelton SI, Pettigrew MM. Capacity of serotype 19A and 15B/C Streptococcus pneumoniae isolates for experimental otitis media: Implications for the conjugate vaccine. Vaccine 2010, 28: 2450-2457. PMID: 20067753, PMCID: PMC2851619, DOI: 10.1016/j.vaccine.2009.12.078.Peer-Reviewed Original ResearchConceptsSerotype 19ASerotype 15B/CChinchilla otitis media modelMiddle ear infectionOtitis media modelDisease prevention effortsStreptococcus pneumoniae isolatesExperimental otitis mediaStreptococcus pneumoniae serotypesQuantitative PCRConjugate vaccineOtitis mediaEar infectionsPneumoniae isolatesVaccine formulationsPneumoniae serotypesSame sequence typeVaccine targetsPrevention effortsPathogenic potentialDifferent serotypesSequence typesSerotypesIsolatesVaccine
2009
Molecular Epidemiology of Pediatric Pneumococcal Empyema from 2001 to 2007 in Utah
Byington CL, Hulten KG, Ampofo K, Sheng X, Pavia AT, Blaschke AJ, Pettigrew M, Korgenski K, Daly J, Mason EO. Molecular Epidemiology of Pediatric Pneumococcal Empyema from 2001 to 2007 in Utah. Journal Of Clinical Microbiology 2009, 48: 520-525. PMID: 20018815, PMCID: PMC2815589, DOI: 10.1128/jcm.01200-09.Peer-Reviewed Original ResearchConceptsUtah childrenPCV-7Nonvaccine serotypesPneumococcal empyemaMolecular epidemiologyStreptococcus pneumoniae serotype 1Pneumococcal conjugate vaccineSerotype 1Sequence typesCommon sequence typesEmpyema ratesSerotype replacementConjugate vaccineSerotype switchReplacement diseasePPE casesEmpyemaChildrenSerotypesHigh rateEpidemiologyLicensureMLSTVaccineIncidenceMultilocus Sequence Typing and Pulsed Field Gel Electrophoresis of Otitis Media Causing Pathogens
Thomas JC, Pettigrew MM. Multilocus Sequence Typing and Pulsed Field Gel Electrophoresis of Otitis Media Causing Pathogens. Methods In Molecular Biology 2009, 493: 179-190. PMID: 18839348, PMCID: PMC3581352, DOI: 10.1007/978-1-59745-523-7_11.Peer-Reviewed Original ResearchConceptsOtitis mediaMolecular epidemiologyMultilocus sequence typingMoraxella catarrhalisHaemophilus influenzaeStreptococcus pneumoniaeSequence typingPulsed-Field Gel ElectrophoresisEpidemiologyBacterial pathogensField gel electrophoresisType of studyResearch settingsCharacterization of isolatesPathogensCatarrhalisInfluenzaePneumoniae
2008
Microbial Interactions during Upper Respiratory Tract Infections - Volume 14, Number 10—October 2008 - Emerging Infectious Diseases journal - CDC
Pettigrew MM, Gent JF, Revai K, Patel JA, Chonmaitree T. Microbial Interactions during Upper Respiratory Tract Infections - Volume 14, Number 10—October 2008 - Emerging Infectious Diseases journal - CDC. Emerging Infectious Diseases 2008, 14: 1584-1591. PMID: 18826823, PMCID: PMC2609881, DOI: 10.3201/eid1410.080119.Peer-Reviewed Original ResearchMeSH KeywordsChild, PreschoolCohort StudiesFemaleHaemophilus InfectionsHaemophilus influenzaeHumansInfantMaleMoraxella catarrhalisMoraxellaceae InfectionsNasopharynxOtitis MediaProspective StudiesRespiratory Tract InfectionsStaphylococcal InfectionsStaphylococcus aureusStreptococcus pneumoniaeSuperinfectionConceptsUpper respiratory tract infectionH. influenzaeInfectious Diseases journal - CDCRespiratory tract infectionsS. pneumoniae colonizationHealthy children 6Months of ageS. aureusProspective cohortTract infectionsPneumoniae colonizationVaccination strategiesAntimicrobial therapyMoraxella catarrhalisChildren 6M. catarrhalisS. pneumoniaeHaemophilus influenzaeStreptococcus pneumoniaeBacterial infectionsSpecific bacterial speciesInfluenzaeLikelihood of colonizationCatarrhalisStaphylococcus aureus
2006
Variation in the Presence of Neuraminidase Genes among Streptococcus pneumoniae Isolates with Identical Sequence Types
Pettigrew MM, Fennie KP, York MP, Daniels J, Ghaffar F. Variation in the Presence of Neuraminidase Genes among Streptococcus pneumoniae Isolates with Identical Sequence Types. Infection And Immunity 2006, 74: 3360-3365. PMID: 16714565, PMCID: PMC1479257, DOI: 10.1128/iai.01442-05.Peer-Reviewed Original ResearchMeSH KeywordsBacterial Typing TechniquesChild, PreschoolGenetic VariationHumansInfantInfant, NewbornNeuraminidaseSequence Analysis, DNAStreptococcus pneumoniaeVirulenceConceptsPneumoniae isolatesSequence typesUpper respiratory tractNeuraminidase geneS. pneumoniae isolatesVirulence determinantsStreptococcus pneumoniae isolatesIdentical sequence typesYoung childrenAdditional virulence determinantsSialic acid-containing substratesCapsule typesMultilocus sequence typesOtitis mediaInvasive diseaseRespiratory tractCarriage isolatesPneumococcal clonesSame sequence typePneumococcal strainsImportant causeStreptococcus pneumoniaeMiddle earDifferent tissue sourcesPneumococcal neuraminidase
2005
Genomic Subtraction Followed by Dot Blot Screening of Streptococcus pneumoniae Clinical and Carriage Isolates Identifies Genetic Differences Associated with Strains That Cause Otitis Media
Pettigrew MM, Fennie KP. Genomic Subtraction Followed by Dot Blot Screening of Streptococcus pneumoniae Clinical and Carriage Isolates Identifies Genetic Differences Associated with Strains That Cause Otitis Media. Infection And Immunity 2005, 73: 2805-2811. PMID: 15845484, PMCID: PMC1087362, DOI: 10.1128/iai.73.5.2805-2811.2005.Peer-Reviewed Original ResearchConceptsMiddle ear isolatesEar isolatesOtitis mediaMiddle earOtitis media pathogenesisCerebrospinal fluid isolatesBacterial otitis mediaStreptococcus pneumoniae strainsMolecular epidemiological approachSpecific bacterial factorsCarriage isolatesPneumoniae strainsBacterial factorsDot-blot screeningBloodBrucella melitensisEpidemiological approachMeningitisFurther studiesYoung childrenPrecise roleCarriageMedium virulenceIsolatesEar
2003
Molecular epidemiology of Streptococcus pneumoniae mediated otitis media.
McCoy S, Pettigrew M. Molecular epidemiology of Streptococcus pneumoniae mediated otitis media. Frontiers In Bioscience-Landmark 2003, 8: e87-93. PMID: 12456346, DOI: 10.2741/961.Peer-Reviewed Original ResearchMeSH KeywordsHumansMolecular EpidemiologyOtitis MediaPneumococcal InfectionsStreptococcus pneumoniae