2018
On the damage done to the structure of the Thermoplasma acidophilum proteasome by electron radiation
Wang J, Liu Z, Crabtree RH, Frank J, Moore PB. On the damage done to the structure of the Thermoplasma acidophilum proteasome by electron radiation. Protein Science 2018, 27: 2051-2061. PMID: 30242932, PMCID: PMC6237698, DOI: 10.1002/pro.3511.Peer-Reviewed Original ResearchMeSH KeywordsElectronsModels, MolecularProteasome Endopeptidase ComplexProtein ConformationThermoplasmaConceptsLocal chemical effectsElectron beamElectron radiationChemical mechanismStructure effectsChemical effectsElectron microscopeBackbone atomsChemical damageΑ-helixThermoplasma acidophilumMoleculesDose-dependent degradationQuaternary structureElectronsBeamAtomsEM mapsRadiationStructureMicroscopeResolutionSmall changesAcidophilum
2017
Experimental charge density from electron microscopic maps
Wang J. Experimental charge density from electron microscopic maps. Protein Science 2017, 26: 1619-1626. PMID: 28543856, PMCID: PMC5521614, DOI: 10.1002/pro.3198.Peer-Reviewed Original Research
2016
Destruction‐and‐diffraction by X‐ray free‐electron laser
Wang J. Destruction‐and‐diffraction by X‐ray free‐electron laser. Protein Science 2016, 25: 1585-1592. PMID: 27262052, PMCID: PMC5338243, DOI: 10.1002/pro.2959.Peer-Reviewed Original ResearchConceptsX-ray free-electron lasersFree-electron laserSingle femtosecond pulseAtomic scattering factorsFemtosecond pulsesRecent theoretical studiesScattering factorsSuch pulsesElectronic processesDifferent atomsCrystallographic data collectionRadiation damageSingle pulseX-rayPulsesLaserSuch crystalsTheoretical studyCrystalsDiffractionMacromolecular crystalsFemtosecondExperimental evidenceAtomsRadiation
2009
Combining electron crystallography and X-ray crystallography to study the MlotiK1 cyclic nucleotide-regulated potassium channel
Clayton GM, Aller SG, Wang J, Unger V, Morais-Cabral JH. Combining electron crystallography and X-ray crystallography to study the MlotiK1 cyclic nucleotide-regulated potassium channel. Journal Of Structural Biology 2009, 167: 220-226. PMID: 19545635, PMCID: PMC2922748, DOI: 10.1016/j.jsb.2009.06.012.Peer-Reviewed Original ResearchConceptsS1-S4 domainMembrane protein structure determinationTetrameric cation channelsX-ray crystallographyProtein structure determinationPotassium channelsElectron crystallographyMlotiK1Cation channelsX-ray structureNew insightsProjection mapStructure determinationCrystallographyCombined applicationDomainUseful strategyQuality crystals