Aimilia Krypotou
Associate Research Scientist in Microbial PathogenesisDownloadHi-Res Photo
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Microbial Pathogenesis
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Associate Research Scientist in Microbial Pathogenesis
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Microbial Pathogenesis
Associate Research ScientistPrimary
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Frequent collaborators of Aimilia Krypotou's published research.
Publications Timeline
A big-picture view of Aimilia Krypotou's research output by year.
Eduardo Groisman, PhD
Andrew Goodman, PhD
Jun Liu, PhD
Nick Pokorzynski, PhD
Shoichi Tachiyama
11Publications
349Citations
Publications
2023
Advancing the fitness of gut commensal bacteria
Groisman E, Han W, Krypotou E. Advancing the fitness of gut commensal bacteria. Science 2023, 382: 766-768. PMID: 37972163, PMCID: PMC10838159, DOI: 10.1126/science.adh9165.Peer-Reviewed Original ResearchCitationsAltmetricBacteria require phase separation for fitness in the mammalian gut
Krypotou E, Townsend G, Gao X, Tachiyama S, Liu J, Pokorzynski N, Goodman A, Groisman E. Bacteria require phase separation for fitness in the mammalian gut. Science 2023, 379: 1149-1156. PMID: 36927025, PMCID: PMC10148683, DOI: 10.1126/science.abn7229.Peer-Reviewed Original ResearchCitationsAltmetricMeSH Keywords and ConceptsConceptsMammalian gutTranscription termination factor RhoTermination factor RhoGene regulationTranscription terminationMechanisms bacteriaBacteria interactionsHuman commensalValuable targetBacteriaRhoGut microbiotaFitnessNovel clinical applicationsTherapeutic manipulationGutHuman healthCommensalRegulation
2019
Control of Bacterial Virulence through the Peptide Signature of the Habitat
Krypotou E, Scortti M, Grundström C, Oelker M, Luisi B, Sauer-Eriksson A, Vázquez-Boland J. Control of Bacterial Virulence through the Peptide Signature of the Habitat. Cell Reports 2019, 26: 1815-1827.e5. PMID: 30759392, PMCID: PMC6389498, DOI: 10.1016/j.celrep.2019.01.073.Peer-Reviewed Original ResearchCitationsAltmetricMeSH Keywords and ConceptsConceptsBacterial virulenceControl of bacterial virulenceFacultative intracellular bacterium Listeria monocytogenesOpp transport systemIntracellular bacterium Listeria monocytogenesBacterium Listeria monocytogenesGSH-binding siteVirulence programCo-crystallization studiesNutritional peptidesPrfAGenetic screeningTranscriptional activityOrganic N sourcesVirulenceNon-cysteine-containing peptidesSteric blockadeHost entryRegulation mechanismBinding sitesPeptide signaturesActive peptidesPeptideN sourcesOligopeptides
2017
Listeria Placental Infection
Vázquez-Boland J, Krypotou E, Scortti M. Listeria Placental Infection. MBio 2017, 8: 10.1128/mbio.00949-17. PMID: 28655824, PMCID: PMC5487735, DOI: 10.1128/mbio.00949-17.Peer-Reviewed Original ResearchCitationsAltmetricMeSH Keywords and ConceptsConceptsCongenital neonatal infectionsAdverse pregnancy outcomesAbsence of overt illnessSystemic bacterial infectionNonhuman primate modelFetal complicationsCausative agent of listeriosisNeonatal infectionPreterm birthPregnancy outcomesPregnant womenFood-borne infectionsBacterial infectionsPrimate modelTransplacental infectionInfectionOvert illnessMedical interventionsCausative agentListeriosisComplicationsMiscarriageStillbirthPathophysiologyBirth
2016
Design and synthesis of purine analogues as highly specific ligands for FcyB, a ubiquitous fungal nucleobase transporter
Lougiakis N, Gavriil E, Kairis M, Sioupouli G, Lambrinidis G, Benaki D, Krypotou E, Mikros E, Marakos P, Pouli N, Diallinas G. Design and synthesis of purine analogues as highly specific ligands for FcyB, a ubiquitous fungal nucleobase transporter. Bioorganic & Medicinal Chemistry 2016, 24: 5941-5952. PMID: 27720327, DOI: 10.1016/j.bmc.2016.09.055.Peer-Reviewed Original ResearchCitationsMeSH Keywords and Concepts
2015
Origin, diversification and substrate specificity in the family of NCS1/FUR transporters
Krypotou E, Evangelidis T, Bobonis J, Pittis A, Gabaldón T, Scazzocchio C, Mikros E, Diallinas G. Origin, diversification and substrate specificity in the family of NCS1/FUR transporters. Molecular Microbiology 2015, 96: 927-950. PMID: 25712422, DOI: 10.1111/mmi.12982.Peer-Reviewed Original ResearchCitationsAltmetricMeSH Keywords and ConceptsMeSH KeywordsAmino Acid SequenceAspergillus nidulansBinding SitesFungal ProteinsGene DuplicationGene Transfer, HorizontalMembrane Transport ProteinsMolecular Docking SimulationMolecular Dynamics SimulationMutationPhylogenyProtein ConformationProtein Structure, TertiaryPseudogenesSequence Homology, Amino AcidSubstrate SpecificitySymportersConceptsSubstrate specificitySubstrate binding siteFunctional diversificationModel fungus Aspergillus nidulansSystematic mutational analysisBinding sitesStructure-function analysisUptake of purinesNCS1 proteinsPlant homologuesFur proteinAspergillus nidulansGene duplicationHorizontal transferSubstrate dockingMutation analysisSub-familyHomology modelingMolecular mechanismsNCS1ProteinResiduesFurDiversificationNidulansFunctional characterization of NAT/NCS2 proteins of Aspergillus brasiliensis reveals a genuine xanthine–uric acid transporter and an intrinsically misfolded polypeptide
Krypotou E, Scazzocchio C, Diallinas G. Functional characterization of NAT/NCS2 proteins of Aspergillus brasiliensis reveals a genuine xanthine–uric acid transporter and an intrinsically misfolded polypeptide. Fungal Genetics And Biology 2015, 75: 56-63. PMID: 25639910, DOI: 10.1016/j.fgb.2015.01.009.Peer-Reviewed Original ResearchCitationsMeSH Keywords and ConceptsConceptsNucleobase-ascorbate transporterUptake of purinesAcid transportAspergillus nidulansMisfolded polypeptidesER-retainedGFP tagMisfolded proteinsHeterologous expressionEvolutionary implicationsInactive proteinPutative transportersAspergillus brasiliensisFunctional characterizationTurned-overPlasma membranePeptide sequencesIn silicoProteinSubfamilyAspergillusTransport functionLow affinityHigher affinityVacuoles
2014
Modelling, substrate docking and mutational analysis identify residues essential for function and specificity of the major fungal purine transporter AzgA
Krypotou E, Lambrinidis G, Evangelidis T, Mikros E, Diallinas G. Modelling, substrate docking and mutational analysis identify residues essential for function and specificity of the major fungal purine transporter AzgA. Molecular Microbiology 2014, 93: 129-145. PMID: 24818808, DOI: 10.1111/mmi.12646.Peer-Reviewed Original ResearchCitationsMeSH Keywords and ConceptsConceptsSubstrate dockingMutation analysisNucleobase-ascorbate transporterAmino acid residuesNAT familyAspergillus nidulansInverted repeatsSubstrate bindingSubstrate translocationA-helicesPurine bindingC-tailAcid residuesTransporter familyCytoplasmic N-H(+) bindingAzgATMS8TMS10Founding memberFlexible domainsBinding cavityGate domainTMS3Residues
2013
Transport assays in filamentous fungi: Kinetic characterization of the UapC purine transporter of Aspergillus nidulans
Krypotou E, Diallinas G. Transport assays in filamentous fungi: Kinetic characterization of the UapC purine transporter of Aspergillus nidulans. Fungal Genetics And Biology 2013, 63: 1-8. PMID: 24355588, DOI: 10.1016/j.fgb.2013.12.004.Peer-Reviewed Original ResearchCitationsMeSH Keywords and ConceptsConceptsFilamentous ascomycete Aspergillus nidulansUric acid-xanthine transporterSpecificity profileAscomycete Aspergillus nidulansTransport assaysNucleobase-ascorbate transporter familySubstrate specificity profileMeasured apparent KmGenetic null mutantsAspergillus nidulansA. nidulansNull mutantsFilamentous fungiGerminating conidiosporesTransporter familyKinetic characterizationGenetic backgroundCompetition assaysVmax valuesMaximal expressionApparent KmUapCMorphological stagesAssayKinetic analysisTransport Assays in Aspergillus nidulans
Krypotou E, Diallinas G. Transport Assays in Aspergillus nidulans. Bio-protocol 2013, 3 DOI: 10.21769/bioprotoc.971.Peer-Reviewed Original Research