activity Reviewer
07/28/2022 - PresentDescriptionReceived invitation from Molecular Omics journal. Acting as an independent reviewer for the journalactivity Member
02/23/2022 - Presentactivity Member
2022 - Presentactivity Member
2022 - Presentactivity Hemoglobin normalization outperforms other methods for standardizing dried blood spot metabolomics: A comparative study
honor ECR Best Poster Award in “MANA 2022” Metabolomics Association of North America
honor MANA 2022” conference travel award by Metabolomics Association of North America
activity Hemoglobin normalization outperforms other methods for standardizing dried blood spot metabolomics: A comparative study
activity Hemoglobin normalization outperforms other methods for standardizing dried blood spot metabolomics: A comparative study
honor Metabolomics Society Early Career Travel Awards
honor EMN Award 2022
activity Member
2019 - 2021activity Student C0-Advisor
2019 - 2021DescriptionServed as a co-advisor for the thesis of 2 graudate studentsactivity Novel mass spectrometry-based metabolomics tool for the selective investigation of gut microbiota-derived metabolites as biomarkers of diet
Abstract/SynopsisThe 14th International Scientific Conference on Probiotics, Prebiotics, Gut Microbiota and Health. The gut microbiome converts dietary compounds that are absorbed in the gastrointestinal tract and further metabolized by the human host. Sulfated metabolites are a major compound class derived from this co-metabolism and have been linked to disease development. In the present multidisciplinary study, we have investigated human urine samples from a dietary intervention study with 22 individuals collected before and after consumption of a polyphenol rich breakfast. These samples were analyzed utilizing our method combining enzymatic metabolite hydrolysis using an arylsulfatase and mass spectrometric metabolomics. Key to this study is the validation of 235 structurally diverse sulfated metabolites. We have identified 48 significantly upregulated metabolites upon dietary intervention including 11 previously unknown sulfated metabolites for this diet. We observed a large variation in subjects based on their potential to sulfate metabolites, which may be the foundation for classification of subjects as high and low sulfate metabolizers in future large cohort studies. The reported sulfatase-based method is a robust tool for the discovery of unknown microbiota-derived metabolites in human samples.
activity Instructor
05/14/2020 - 05/16/2020Description1. Presented metabolomics lectures on XCMS Online and Multivariate statistical Analysis to a class of 20 students 2. Presented lecture on metabolite quantificationactivity Novel mass spectrometry-based metabolomics tools for the selective investigation of gut microbiota-derived metabolites
Abstract/SynopsisNordic meeting in molecular microbial ecology 2019
honor Rector's research allowance from the Wallenberg Foundation
honor Apotekare C D Carlssons stiftelse, Swedish pharmaceutical society
honor Elisabeth and Alfred Ahlqvist's foundation scholarship ,Swedish pharmaceutical society
activity Involvement of organic acids and amino acids in ameliorating Ni (II) toxicity induced cell cycle dysregulation in Caulobacter crescentus: a metabolomics analysis
Abstract/Synopsismicrobiology society annual conference. Nickel (Ni(II)) toxicity is addressed by many different bacteria, but bacterial responses to nickel stress are still unclear. Therefore, we studied the effect of Ni(II) toxicity on cell proliferation of α-proteobacterium Caulobacter crescentus. Next, we showed the mechanism that allows C. crescentus to survive in Ni(II) stress condition. Our results revealed that the growth of C. crescentus is severely affected when the bacterium was exposed to different Ni(II) concentrations, 0.003 mM slightly affected the growth, 0.008 mM reduced the growth by 50%, and growth was completely inhibited at 0.015 mM. It was further shown that Ni(II) toxicity induced mislocalization of major regulatory proteins such as MipZ, FtsZ, ParB, and MreB, resulting in dysregulation of the cell cycle. GC-MS metabolomics analysis of Ni(II) stressed C. crescentus showed an increased level of nine important metabolites including TCA cycle intermediates and amino acids. This indicates that changes in central carbon metabolism and nitrogen metabolism are linked with the disruption of cell division process. Addition of malic acid, citric acid, alanine, proline, and glutamine to 0.015 mM Ni(II)-treated C. crescentus restored its growth. Thus, the present work shows a protective effect of these organic acids and amino acids on Ni(II) toxicity. Metabolic stimulation through the PutA/GlnA pathway, accelerated degradation of CtrA, and Ni-chelation by organic acids or amino acids are some of the possible mechanisms suggested to be involved in enhancing C. crescentus's tolerance. Our results shed light on the mechanism of increased Ni(II) tolerance in C. crescentus which may be useful in bioremediation strategies and synthetic biology applications such as the development of whole cell biosensor.