2012
169
Oda S, Hirasawa H, Sumpio B. 169. Critical Care Medicine 2012, 40: 1-328. DOI: 10.1097/01.ccm.0000424389.66537.91.Peer-Reviewed Original ResearchTF RNA expressionHigh TF expressionTF expressionTissue factorEndothelial cellsChemical stimulationRNA expressionExcessive inflammatory reactionReal-time RT-PCRLaminar flowVascular endothelial cellsQuantitative real-time RT-PCRHemodynamic stabilizationIntravascular activationIntravascular thrombosisFro flowInflammatory reactionExtrinsic coagulationTumor growth
2011
High Throughput Determination of TGFβ1/SMAD3 Targets in A549 Lung Epithelial Cells
Zhang Y, Handley D, Kaplan T, Yu H, Bais AS, Richards T, Pandit KV, Zeng Q, Benos PV, Friedman N, Eickelberg O, Kaminski N. High Throughput Determination of TGFβ1/SMAD3 Targets in A549 Lung Epithelial Cells. PLOS ONE 2011, 6: e20319. PMID: 21625455, PMCID: PMC3098871, DOI: 10.1371/journal.pone.0020319.Peer-Reviewed Original ResearchMeSH KeywordsBase SequenceCell LineChromatin ImmunoprecipitationDNA PrimersElectrophoretic Mobility Shift AssayEpithelial CellsHumansLungOligonucleotide Array Sequence AnalysisPromoter Regions, GeneticProtein BindingReverse Transcriptase Polymerase Chain ReactionSmad3 ProteinTransforming Growth Factor beta1ConceptsGene expression microarraysLung epithelial cellsMolecular pathwaysTranscriptional regulationExpression microarraysGlobal transcriptional regulationTGFβ1/Smad3Epithelial cellsHuman promoter regionsSignal transduction cascadeTarget gene expressionEpithelial cell phenotypeGene expression analysisTranscription factor Smad3Primary lung epithelial cellsSmad3 targetsQuantitative real-time RT-PCRFOXA2 promoterHuman A549 alveolar epithelial cellsChromatin immunoprecipitationTransduction cascadeTarget genesA549 lung epithelial cellsExpression analysisGene expression
2009
Progestin and Thrombin Regulate Tissue Factor Expression in Human Term Decidual Cells
Lockwood CJ, Murk W, Kayisli UA, Buchwalder LF, Huang S, Funai EF, Krikun G, Schatz F. Progestin and Thrombin Regulate Tissue Factor Expression in Human Term Decidual Cells. The Journal Of Clinical Endocrinology & Metabolism 2009, 94: 2164-2170. PMID: 19276228, PMCID: PMC2690421, DOI: 10.1210/jc.2009-0065.Peer-Reviewed Original ResearchConceptsMaternal-fetal interfaceTerm decidual cellsDecidual cellsTNF-alphaTissue factorPlacental sectionsTF mRNA levelsIL-1betaHuman maternal-fetal interfaceTF expressionWestern blottingTF levelsVillous mesenchymal cellsRT-PCRTerm placental sectionsMRNA levelsHigher histologic scoresTissue factor expressionIL-1beta effectReal-time RT-PCRQuantitative real-time RT-PCRDecidual sectionsHemostatic protectionPreterm deliveryQuantitative RT-PCRWNT5A Is a Regulator of Fibroblast Proliferation and Resistance to Apoptosis
Vuga LJ, Ben-Yehudah A, Kovkarova-Naumovski E, Oriss T, Gibson KF, Feghali-Bostwick C, Kaminski N. WNT5A Is a Regulator of Fibroblast Proliferation and Resistance to Apoptosis. American Journal Of Respiratory Cell And Molecular Biology 2009, 41: 583-589. PMID: 19251946, PMCID: PMC2778165, DOI: 10.1165/rcmb.2008-0201oc.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsApoptosisbeta CateninBlotting, WesternCase-Control StudiesCaspase 3Cell LineCell ProliferationCell SurvivalFibroblastsFibronectinsGene Expression ProfilingHumansHydrogen PeroxideIdiopathic Pulmonary FibrosisIntegrin alpha5LungMiceOligonucleotide Array Sequence AnalysisProto-Oncogene ProteinsRecombinant ProteinsReverse Transcriptase Polymerase Chain ReactionRNA InterferenceTransfectionUp-RegulationWnt ProteinsWnt-5a ProteinConceptsUsual interstitial pneumoniaNormal lung fibroblastsLung tissueLung fibroblastsFibrotic interstitial lung diseaseInterstitial lung fibrosisSpecific histopathologic patternIdiopathic pulmonary fibrosisInterstitial lung diseaseRole of Wnt5aReal-time RT-PCRQuantitative real-time RT-PCRInterstitial pneumoniaPulmonary fibrosisAutoimmune diseasesHistopathologic patternLung diseaseLung fibrosisHistological patternNormal histologyWnt/beta-catenin pathwayCanonical Wnt/beta-catenin pathwayWestern blotFibroblast proliferationBeta-catenin pathway
2008
Experimental Murine Endometriosis Induces DNA Methylation and Altered Gene Expression in Eutopic Endometrium1
Lee B, Du H, Taylor HS. Experimental Murine Endometriosis Induces DNA Methylation and Altered Gene Expression in Eutopic Endometrium1. Biology Of Reproduction 2008, 80: 79-85. PMID: 18799756, PMCID: PMC2804809, DOI: 10.1095/biolreprod.108.070391.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsDNA MethylationEndometriosisEndometriumFemaleGene Expression RegulationHomeobox A10 ProteinsHomeodomain ProteinsImmunohistochemistryInsulin-Like Growth Factor Binding Protein 1Integrin beta ChainsKruppel-Like Transcription FactorsMiceReceptors, ProgesteroneReverse Transcriptase Polymerase Chain ReactionRNA, MessengerConceptsEndometriosis groupEndometrial receptivityEutopic endometriumExperimental endometriosisAltered gene expressionInsulin-like growth factorInduction of endometriosisKruppel-like factor 9Expression of HOXA10Total progesterone receptorEndometrial gene expressionBeta3 mRNA expressionProtein-1 mRNAReal-time RT-PCRQuantitative real-time RT-PCRGene expressionMurine endometriosisNormal endometriumProgesterone receptorMethylation-specific PCREndometriosisEndometriumMRNA expressionEctopic locationsGrowth factorCalpain5 expression is decreased in endometriosis and regulated by HOXA10 in human endometrial cells
Penna I, Du H, Ferriani R, Taylor HS. Calpain5 expression is decreased in endometriosis and regulated by HOXA10 in human endometrial cells. Molecular Human Reproduction 2008, 14: 613-618. PMID: 18829447, PMCID: PMC2734161, DOI: 10.1093/molehr/gan055.Peer-Reviewed Original ResearchConceptsEndometrial cellsGlandular cellsDevelopment of endometriosisHuman endometrial cellsExpression of HOXA10Real-time RT-PCRQuantitative real-time RT-PCRHOXA10 siRNAEndometrial biopsyEpithelial cell lineDecidual samplesEutopic endometriumMenstrual cyclePregnancy terminationEndometriosisFertile controlsHOXA10 expressionDecreased expressionRT-PCRProtein expressionRespective controlsHOXA10BiopsyCell linesAberrant regulationTherapeutic siRNAs for dominant genetic skin disorders including pachyonychia congenita
Leachman SA, Hickerson RP, Hull PR, Smith FJ, Milstone LM, Lane EB, Bale SJ, Roop DR, McLean WH, Kaspar RL. Therapeutic siRNAs for dominant genetic skin disorders including pachyonychia congenita. Journal Of Dermatological Science 2008, 51: 151-157. PMID: 18495438, PMCID: PMC2587483, DOI: 10.1016/j.jdermsci.2008.04.003.Peer-Reviewed Original ResearchConceptsPachyonychia congenitaQuantitative RT-PCRRT-PCRReal-time RT-PCRQuantitative real-time RT-PCRClinical efficacyGenetic skin disordersClinical trialsCandidate siRNASkin disordersRNA interferenceNovel therapeuticsPotent inhibitionTherapeutic siRNAsClinical applicationDisordersGenetic disordersPhysiciansCongenitaMutant allelesSiRNAHuman diseasesKeratin disordersPatientsDisease
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