2024
A compact regulatory RNA element in mouse Hsp70 mRNA
Wang W, Liu F, Ugalde M, Pyle A. A compact regulatory RNA element in mouse Hsp70 mRNA. NAR Molecular Medicine 2024, 1: ugae002. PMID: 38318492, PMCID: PMC10840451, DOI: 10.1093/narmme/ugae002.Peer-Reviewed Original ResearchOpen reading frameRNA elementsHeat shockOpen reading frame sequenceRegulatory RNA elementsSecondary structure modelMessenger RNACap-independent mannerCap-dependent translationMolecular chaperone functionSequences of HSP70Reading frameMisfolded proteinsRNA foldingRegulate protein expressionChaperone functionMRNA sequencesUntranslated regionShort stretchSecondary structureCell stressGene expressionHSP70 gene expressionStructural basisMolecular mechanisms
2018
Elongation factor P controls translation of the mgtA gene encoding a Mg2+ transporter during Salmonella infection
Choi E, Nam D, Choi J, Park S, Lee J, Lee E. Elongation factor P controls translation of the mgtA gene encoding a Mg2+ transporter during Salmonella infection. MicrobiologyOpen 2018, 8: e00680. PMID: 29949242, PMCID: PMC6460261, DOI: 10.1002/mbo3.680.Peer-Reviewed Original ResearchConceptsMgtA geneProline codonsControl translationIntramacrophage survivalPathogen Salmonella enterica serovar TyphimuriumElongation factor PConsecutive proline codonsOpen reading frameSalmonella enterica serovar TyphimuriumEnterica serovar TyphimuriumTranslational controlReading frameSalmonella pathogenesisMRNA sequencesGenesCodonSerovar TyphimuriumRibosomesFactor P
2016
Development of a Quantitative PCR Assay for Detection of Human Insulin-Like Growth Factor Receptor and Insulin Receptor Isoforms
Flannery CA, Rowzee AM, Choe GH, Saleh FL, Radford CC, Taylor HS, Wood TL. Development of a Quantitative PCR Assay for Detection of Human Insulin-Like Growth Factor Receptor and Insulin Receptor Isoforms. Endocrinology 2016, 157: 1702-1708. PMID: 26862994, PMCID: PMC4816738, DOI: 10.1210/en.2015-1698.Peer-Reviewed Original ResearchConceptsSplice variantsInsulin-like growth factor (IGF) ligandsInsulin-like growth factor receptorGrowth factor ligandsSpecific molecular signaturesQuantitative PCR assaysGrowth factor receptorHigh homologyMRNA sequencesQuantitative RT-PCRIGF-1 receptorFactor ligandInsulin receptor isoformsCompetition assaysPrimer pairsRelative abundanceInsulin receptorMolecular signaturesCell typesDifferent tissuesIGF-1R mRNAFactor receptorExpression levelsGel electrophoresisReceptor isoforms
1998
Analyses of Ribosome Distribution During In Vitro Translation
Wolin S. Analyses of Ribosome Distribution During In Vitro Translation. Methods In Molecular Biology 1998, 77: 1-9. PMID: 9770657, DOI: 10.1385/0-89603-397-x:1.Peer-Reviewed Original ResearchConceptsRibosome distributionFull-length cDNA cloneSignal recognition particleArrest of translationSingle-nucleotide precisionPosition of ribosomesDistribution of ribosomesRibosome pausesRecognition particleRibosome stallingParticular mRNA speciesTranslational regulationNucleotide precisionVitro translationCDNA clonesAbundant mRNAsMRNA speciesTermination codonRibosomesMRNA sequencesMRNAAssaysTranslationCodonSpecies
1986
Coding sequence of the precursor of the beta subunit of rat propionyl-CoA carboxylase.
Kraus J, Firgaira F, Novotný J, Kalousek F, Williams K, Williamson C, Ohura T, Rosenberg L. Coding sequence of the precursor of the beta subunit of rat propionyl-CoA carboxylase. Proceedings Of The National Academy Of Sciences Of The United States Of America 1986, 83: 8049-8053. PMID: 3464942, PMCID: PMC386864, DOI: 10.1073/pnas.83.21.8049.Peer-Reviewed Original ResearchConceptsPropionyl-CoA carboxylaseNH2-terminal leader peptideAmino acid sequenceBeta subunitBeta-subunit precursorMature subunitAcid sequenceLeader peptideMitochondrial enzyme propionyl-CoA carboxylaseAmino acidsSubunit precursorOpen reading frameAlpha-helical segmentsEnzyme propionyl-CoA carboxylaseCarboxylaseNH2-terminal residuesFirst helixReading frameDNA sequencesPrecursorsCytoplasmic precursorMRNA sequencesArginine residuesHydrophobic momentMRNA transcripts
1985
Light chain germ‐line genes and the immune response to 2‐phenyloxazolone.
Even J, Griffiths G, Berek C, Milstein C. Light chain germ‐line genes and the immune response to 2‐phenyloxazolone. The EMBO Journal 1985, 4: 3439-3445. PMID: 3937727, PMCID: PMC554682, DOI: 10.1002/j.1460-2075.1985.tb04102.x.Peer-Reviewed Original ResearchDideoxy mRNA Sequencing as a Method to Analyze Antibody Diversity
Griffiths G, Berek C, Kaartinen M, Milstein C. Dideoxy mRNA Sequencing as a Method to Analyze Antibody Diversity. Methodological Surveys In Biochemistry And Analysis 1985, 141-142. DOI: 10.1007/978-1-4684-5006-4_17.Peer-Reviewed Original ResearchSequence informationAb diversityNucleotide sequence informationAmino acid sequenceHapten 2-phenyloxazoloneAcid sequenceHeavy chain variable regionMRNA sequencesVariable regionAntibody diversityChain variable regionV region expressionSequenceDiversityAntigen-SpecificImmune responseNucleotideDideoxyAminoHybridoma
1984
Somatic mutation and the maturation of immune response to 2-phenyl oxazolone
Griffiths G, Berek C, Kaartinen M, Milstein C. Somatic mutation and the maturation of immune response to 2-phenyl oxazolone. Nature 1984, 312: 271-275. PMID: 6504141, DOI: 10.1038/312271a0.Peer-Reviewed Original ResearchmRNA sequences define an unusually restricted IgG response to oxazolone
Kaartinen M, Griffiths G, Milstein C. mRNA sequences define an unusually restricted IgG response to oxazolone. Research In Immunology 1984, 135: 143-148. PMID: 6712158, DOI: 10.1016/s0769-2625(84)80024-0.Peer-Reviewed Original Research
1983
Isolation of stable mouse cell lines that express cell surface and secreted forms of the vesicular stomatitis virus glycoprotein.
Florkiewicz R, Smith A, Bergmann J, Rose J. Isolation of stable mouse cell lines that express cell surface and secreted forms of the vesicular stomatitis virus glycoprotein. Journal Of Cell Biology 1983, 97: 1381-1388. PMID: 6415065, PMCID: PMC2112694, DOI: 10.1083/jcb.97.5.1381.Peer-Reviewed Original ResearchConceptsVesicular stomatitis virus glycoproteinMouse cell linesG proteinsNormal G proteinsStable mouse cell linesEndoplasmic reticulumCell linesTg proteinRough endoplasmic reticulumVesicular stomatitis virus G proteinCell surfaceVirus G proteinBovine papilloma virusVirus glycoproteinComplex oligosaccharidesAnchor sequenceLevel of expressionPSV2 vectorCDNA encodingAnchorless proteinAberrant splicingDNA fragmentsGolgi apparatusMRNA sequencesRate-limiting stepmRNA sequences define an unusually restricted IgG response to 2-phenyloxazolone and its early diversification
Kaartinen M, Griffiths G, Markham A, Milstein C. mRNA sequences define an unusually restricted IgG response to 2-phenyloxazolone and its early diversification. Nature 1983, 304: 320-324. PMID: 6877353, DOI: 10.1038/304320a0.Peer-Reviewed Original Research
1982
Somatic cell hybridization of growth hormone-producing rat pituitary cells and mouse fibroblasts results in extinction of growth hormone expression via a defect in growth hormone RNA production.
Strobl J, Dannies P, Thompson E. Somatic cell hybridization of growth hormone-producing rat pituitary cells and mouse fibroblasts results in extinction of growth hormone expression via a defect in growth hormone RNA production. Journal Of Biological Chemistry 1982, 257: 6588-6594. PMID: 6176584, DOI: 10.1016/s0021-9258(20)65183-3.Peer-Reviewed Original ResearchConceptsMouse GH geneGH geneHybrid cellsHybrid cell DNAHigh molecular weight precursorNon-pituitary cellsSomatic cell hybridizationMolecular weight precursorMultiple restriction enzymesStructural geneTranscript degradationMature mRNARestriction enzyme analysisIncomplete transcriptsFlanking sequencesParental cell lineTranscriptional blockRestriction enzyme digestionRNA productionSouthern hybridizationCell hybridizationMRNA sequencesFibroblasts resultsGrowth hormone expressionMouse fibroblast cells
1981
Nucleotide sequences of the mRNA's encoding the vesicular stomatitis virus N and NS proteins
Gallione C, Greene J, Iverson L, Rose J. Nucleotide sequences of the mRNA's encoding the vesicular stomatitis virus N and NS proteins. Journal Of Virology 1981, 39: 529-535. PMID: 6268841, PMCID: PMC171363, DOI: 10.1128/jvi.39.2.529-535.1981.Peer-Reviewed Original ResearchConceptsNS proteinsAUG codonReading frameNucleotide sequenceThird AUG codonAmino acidsComplete nucleotide sequenceVesicular stomatitis virus mRNAOpen reading frameSodium dodecyl sulfate-polyacrylamide gelsAnomalous electrophoretic mobilityDodecyl sulfate-polyacrylamide gelsSecond reading frameSulfate-polyacrylamide gelsCDNA clonesTranslational initiationSmall proteinsMRNA sequencesN proteinVirus NPolyadenylic acidVirus mRNACodonProteinMRNA
1980
Complete intergenic and flanking gene sequences from the genome of vesicular stomatitis virus
Rose J. Complete intergenic and flanking gene sequences from the genome of vesicular stomatitis virus. Cell 1980, 19: 415-421. PMID: 6244108, DOI: 10.1016/0092-8674(80)90515-2.Peer-Reviewed Original ResearchConceptsGene sequencesPossible promoter sequenceFrame AUG codonsIntergenic regionTranscription eventsVSV transcriptionPromoter sequencesExtensive homologyMRNA structurePolyadenylation signalNucleotide sequenceAUG codonVesicular stomatitis virusL geneRNA regionsMRNA sequencesVSV genesTerminal sequenceVSV mRNAsN mRNAStomatitis virusG mRNAMRNARibosomesVariable positions
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