Isolation of stable mouse cell lines that express cell surface and secreted forms of the vesicular stomatitis virus glycoprotein.
Florkiewicz R, Smith A, Bergmann J, Rose J. Isolation of stable mouse cell lines that express cell surface and secreted forms of the vesicular stomatitis virus glycoprotein. Journal Of Cell Biology 1983, 97: 1381-1388. PMID: 6415065, PMCID: PMC2112694, DOI: 10.1083/jcb.97.5.1381.Peer-Reviewed Original ResearchConceptsVesicular stomatitis virus glycoproteinMouse cell linesG proteinsNormal G proteinsStable mouse cell linesEndoplasmic reticulumCell linesTg proteinRough endoplasmic reticulumVesicular stomatitis virus G proteinCell surfaceVirus G proteinBovine papilloma virusVirus glycoproteinComplex oligosaccharidesAnchor sequenceLevel of expressionPSV2 vectorCDNA encodingAnchorless proteinAberrant splicingDNA fragmentsGolgi apparatusMRNA sequencesRate-limiting stepIdentification of two distinct regulatory regions adjacent to the human β-interferon gene
Zinn K, Dimaio D, Maniatis T. Identification of two distinct regulatory regions adjacent to the human β-interferon gene. Cell 1983, 34: 865-879. PMID: 6313211, DOI: 10.1016/0092-8674(83)90544-5.Peer-Reviewed Original ResearchConceptsIFN gene expressionBovine papilloma virusRegulatory regionsExpression of deletion mutantsGene expressionMRNA cap sitePoly(I)-poly(CCell linesStable cell linesIndependent cell linesCloned geneStructural geneDeletion mutantsLevel of expressionKinetics of inductionCap siteMouse cellsHuman beta-interferonGenesInduced expressionConstitutive levelsDeletionBeta-interferonExpressionPapilloma virus
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