2025
Bioluminescent imaging to investigate Coxiella burnetii pathogenesis identifies adipose tissue as a host niche for infection
Andrews J, Roy C. Bioluminescent imaging to investigate Coxiella burnetii pathogenesis identifies adipose tissue as a host niche for infection. Infection And Immunity 2025, 93: e00080-25. PMID: 40586810, PMCID: PMC12341371, DOI: 10.1128/iai.00080-25.Peer-Reviewed Original ResearchConceptsBioluminescence imagingIntraperitoneal infectionAdipose tissueIntraperitoneal infection of miceDisease Q feverMile phase IIInfection of miceHost nichesVisceral adipose tissueCulturing cells <i>inBiosafety level 3 containmentIntracellular replicationVirulence differencesQ feverAnimal hostsGram-negativeIntracellular pathogensTracking of bacteriaImmunocompromised miceBiosafety level 2Determination of immunityNMIIMiceStrainNiche
2024
Dual roles for a tick protein disulfide isomerase during the life cycle of the Lyme disease agent
Tang X, Cui Y, Namarra U, Tian X, Rivas-Giorgi F, Fikrig E. Dual roles for a tick protein disulfide isomerase during the life cycle of the Lyme disease agent. MBio 2024, 15: e01754-24. PMID: 39470213, PMCID: PMC11633212, DOI: 10.1128/mbio.01754-24.Peer-Reviewed Original ResearchProtein disulfide isomeraseLyme disease agentDisulfide isomeraseBlood-feeding vectorsExtracellular pathogensGene expressionInvasion of host cellsThiol-disulfide oxidoreductasesDisease agentsGroup of enzymesStages of bacterial infectionVirulence factorsLife cycleChaperone activityMammalian hostsHost cellsBlood-feeding ticksIsomeraseMicrobial infectionsColonized ticksPathogensDiverse infectionsMicrobial invasionInfection of miceVector-borne diseases
2021
A Mosquito AgTRIO Monoclonal Antibody Reduces Early Plasmodium Infection of Mice
Chuang YM, Tang XD, Fikrig E. A Mosquito AgTRIO Monoclonal Antibody Reduces Early Plasmodium Infection of Mice. Infection And Immunity 2021, 90: e00359-21. PMID: 34724388, PMCID: PMC8788779, DOI: 10.1128/iai.00359-21.Peer-Reviewed Original ResearchConceptsMonoclonal antibodiesFuture malaria vaccinesInfection of miceIsotype monoclonal antibodyVector antigensProtective immunityPassive immunizationMalaria vaccinePlasmodium infectionPassive transferProtein monoclonal antibodySignificant protectionSynergistic protectionMiceInfectionAntibodiesFc regionAntiserumVertebrate hostsProtein TrioImmunizationVaccineMalariaAntigenImmunity
2018
A single-point mutation in the RNA-binding protein 6 generates Trypanosoma brucei metacyclics that are able to progress to bloodstream forms in vitro
Shi H, Butler K, Tschudi C. A single-point mutation in the RNA-binding protein 6 generates Trypanosoma brucei metacyclics that are able to progress to bloodstream forms in vitro. Molecular And Biochemical Parasitology 2018, 224: 50-56. PMID: 30055184, PMCID: PMC6147148, DOI: 10.1016/j.molbiopara.2018.07.011.Peer-Reviewed Original Research
2015
Salmonella Infection Drives Promiscuous B Cell Activation Followed by Extrafollicular Affinity Maturation
Di Niro R, Lee SJ, Vander Heiden J, Elsner RA, Trivedi N, Bannock JM, Gupta NT, Kleinstein SH, Vigneault F, Gilbert TJ, Meffre E, McSorley SJ, Shlomchik MJ. Salmonella Infection Drives Promiscuous B Cell Activation Followed by Extrafollicular Affinity Maturation. Immunity 2015, 43: 120-131. PMID: 26187411, PMCID: PMC4523395, DOI: 10.1016/j.immuni.2015.06.013.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntibodies, MonoclonalB-LymphocytesClonal Selection, Antigen-MediatedGerminal CenterImmunoglobulin GLymphocyte ActivationMiceMice, Inbred BALB CMice, Inbred C57BLMice, KnockoutReceptors, Antigen, B-CellSalmonella InfectionsSalmonella typhimuriumSomatic Hypermutation, ImmunoglobulinSpleenConceptsB cell receptorExtrafollicular sitesGerminal centersAffinity maturationInfection of miceB cell responsesB cell activationDetectable antibodiesSomatic hypermutationExtrafollicular responseAntigen microarraysSalmonella infectionAntigen targetsCell activationSalmonella typhimuriumCell responsesBCR specificityFlow cytometryCell receptorMonoclonal antibodiesUndetectable affinityClonal selectionInfectionAntibodiesLaser microdissection
2009
Antibodies against a Tick Protein, Salp15, Protect Mice from the Lyme Disease Agent
Dai J, Wang P, Adusumilli S, Booth CJ, Narasimhan S, Anguita J, Fikrig E. Antibodies against a Tick Protein, Salp15, Protect Mice from the Lyme Disease Agent. Cell Host & Microbe 2009, 6: 482-492. PMID: 19917502, PMCID: PMC2843562, DOI: 10.1016/j.chom.2009.10.006.Peer-Reviewed Original ResearchConceptsArthropod-borne pathogensTick-borne BorreliaTick salivary proteinsTick proteinsB. burgdorferiLyme diseaseDisease agentsTick-borne illnessB. burgdorferi infectionLyme disease agentHuman vaccinesSalp15Infection of miceB. burgdorferi antigensMicrobial toxinsMammalian hostsBorrelia burgdorferiPathogensMechanism of actionBurgdorferi infectionProtect miceMedical importanceBurgdorferiProtective capacityMiceTrypanosoma cruzi Triggers an Early Type I IFN Response In Vivo at the Site of Intradermal Infection
Chessler AD, Unnikrishnan M, Bei AK, Daily JP, Burleigh BA. Trypanosoma cruzi Triggers an Early Type I IFN Response In Vivo at the Site of Intradermal Infection. The Journal Of Immunology 2009, 182: 2288-2296. PMID: 19201883, DOI: 10.4049/jimmunol.0800621.Peer-Reviewed Original ResearchConceptsInfection of miceIFN responseT. cruzi-infected miceIFN-gamma-deficient miceIFN-gamma-producing cellsLevels of IFNEarly type IType I IFN receptorInnate immune responseIntradermal infection modelEarly host responseSite of infectionType I IFNT. cruziI IFN receptorLocal infection siteSite of inoculationType IIntradermal infectionImmune responseI IFNPrimary siteHost responseIFN receptorInfection
2002
Murine Lyme Arthritis Development Mediated by p38 Mitogen-Activated Protein Kinase Activity
Anguita J, Barthold SW, Persinski R, Hedrick MN, Huy CA, Davis RJ, Flavell RA, Fikrig E. Murine Lyme Arthritis Development Mediated by p38 Mitogen-Activated Protein Kinase Activity. The Journal Of Immunology 2002, 168: 6352-6357. PMID: 12055252, PMCID: PMC4309983, DOI: 10.4049/jimmunol.168.12.6352.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntigens, BacterialArthritis, InfectiousBorrelia burgdorferiCD4-Positive T-LymphocytesCell LineEnzyme ActivationInflammationInterferon-gammaLyme DiseaseMAP Kinase Kinase 3MAP Kinase Signaling SystemMiceMice, KnockoutMitogen-Activated Protein Kinase KinasesMitogen-Activated Protein Kinasesp38 Mitogen-Activated Protein KinasesPhagocytesPhosphorylationProtein-Tyrosine KinasesReceptors, InterferonConceptsProinflammatory cytokine productionCytokine productionT helper type 1 responsePhagocytic cellsDevelopment of arthritisPotential new therapeutic approachType 1 responseInfection of miceExperimental murine modelMurine Lyme arthritisNew therapeutic approachesLyme arthritis developmentTreatment of inflammationCytokine burstArthritis developmentJoint inflammationLyme arthritisNF-kappa BProinflammatory cytokinesTNF-alphaT cellsMurine modelTherapeutic approachesP38 MAP kinaseSpecific Abs
2001
Infection of Mice with the Agent of Human Granulocytic Ehrlichiosis after Different Routes of Inoculation
Hodzic E, Feng S, Fish D, Leutenegger C, Freet K, Barthold S. Infection of Mice with the Agent of Human Granulocytic Ehrlichiosis after Different Routes of Inoculation. The Journal Of Infectious Diseases 2001, 183: 1781-1786. PMID: 11372031, DOI: 10.1086/320735.Peer-Reviewed Original ResearchConceptsBone marrow samplesReal-time polymerase chain reactionPolymerase chain reactionHuman granulocytic ehrlichiosisMarrow samplesDay 20Granulocytic ehrlichiosisInfection of miceRate of infectionKinetics of infectionLymph nodesGene targetsInfected miceC3H miceEarly disseminationVascular perfusionDay 10Experimental infectionInfectionMiceNeedle inoculationChain reactionBloodEhrlichiosisInoculation
1995
Cellular mechanisms in the immune response to malaria in Plasmodium vinckei-infected mice
Perlmann H, Kumar S, Vinetz J, Kullberg M, Miller L, Perlmann P. Cellular mechanisms in the immune response to malaria in Plasmodium vinckei-infected mice. Infection And Immunity 1995, 63: 3987-3993. PMID: 7558309, PMCID: PMC173560, DOI: 10.1128/iai.63.10.3987-3993.1995.Peer-Reviewed Original ResearchConceptsImmune miceTh1 responseControl miceIL-4T cellsSpleen cellsIL-4-producing Th2 cellsBALB/c miceTumor necrosis factor alphaAbsence of elevationPlasmodium vinckei vinckeiImmunoglobulin E levelsInfection of miceGamma interferon productionNecrosis factor alphaChabaudi infectionAntigen exposureNonimmune miceImportant protective factorC miceSolid immunityTh2 cellsFactor alphaParasite antigensE levels
1984
Autoimmunity following viral infection: demonstration of monoclonal antibodies against normal tissue following infection of mice with reovirus and demonstration of shared antigenicity between virus and lymphocytes
Tardieu M, Powers M, Hafler D, Hauser S, Weiner H. Autoimmunity following viral infection: demonstration of monoclonal antibodies against normal tissue following infection of mice with reovirus and demonstration of shared antigenicity between virus and lymphocytes. European Journal Of Immunology 1984, 14: 561-565. PMID: 6329771, DOI: 10.1002/eji.1830140614.Peer-Reviewed Original ResearchConceptsNormal tissuesMonoclonal antibodiesViral infectionOnly virusInfection of miceUninfected control animalsAdult C57BL/6 miceAutoreactive monoclonal antibodiesNS1 myeloma cellsReovirus type 3Reovirus type 1Autoimmune responseC57BL/6 miceLung tissueT lymphocytesImmune responseSplenic lymphocytesControl animalsEpendymal cellsViral determinantsMyeloma cellsType 1LymphocytesInfectionReovirus type
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