2024
PLK1 Acts in Homologous Recombinatorial Repair and in Mitosis As Synthetically Lethal with the Fanconi Anemia/BRCA Pathway
Kupfer G, Doroodian P, Li J, Golemis E, Young P, Stahl E, Nagarajan A, Sung P, Burtness B. PLK1 Acts in Homologous Recombinatorial Repair and in Mitosis As Synthetically Lethal with the Fanconi Anemia/BRCA Pathway. Blood 2024, 144: 2705-2705. DOI: 10.1182/blood-2024-200980.Peer-Reviewed Original ResearchHead and neck squamous cell carcinomaMutant cell typesLow dose treatmentMutant cellsHR repair efficiencyCell cycle analysisFA mutationsFanconi anemiaBone marrow failure syndromesCorrected cellsNeck squamous cell carcinomaDose treatmentPARP1 inhibitorsHypersensitivity to DNA-damaging agentsPLK1 expressionCombination Targeted TherapyIncreased risk of cancerFA gene mutationsMarrow failure syndromesMitomycin C (MMCSubstrate of Plk1DNA interstrand crosslinking agentsNon-FA patientsRAD51 fociSquamous cell carcinoma
2021
Immune-Mediated Reprogramming of Intestinal Stem Cells Drives STAT1-Dependent Myc Expression and Epithelial Regeneration in GI-Gvhd
Takashima S, Sharma R, Egorova A, Kuttiyara J, Ito T, Chang W, Jansen S, Liu C, Lindemans C, Mazutis L, Robine N, Hanash A. Immune-Mediated Reprogramming of Intestinal Stem Cells Drives STAT1-Dependent Myc Expression and Epithelial Regeneration in GI-Gvhd. Blood 2021, 138: 86-86. DOI: 10.1182/blood-2021-152647.Peer-Reviewed Original ResearchIntestinal stem cellsGene Set Enrichment AnalysisMYC expressionIFNG treatmentSTAT1-dependent mannerActivating myc expressionDownstream of Wnt signalingInduced epithelial apoptosisInhibitor 10058-F4Intestinal stem cell maintenanceMYC inhibitor 10058-F4Single cell RNA sequencingIFNG signalingIntestinal stem cell populationIntestinal cryptsCell RNA sequencingGene expression profilesCell cycle analysisUpregulated transcription factorsWnt target gene Axin2Myc functionUpregulation of cyclin D1Sequenced cellsMYC pathwayPhenotypic distancesHDAC Inhibition Induces Cell Cycle Arrest and Mesenchymal-Epithelial Transition in a Novel Pleural-Effusion Derived Uterine Carcinosarcoma Cell Line
Stockhammer P, Okumus Ö, Hegedus L, Rittler D, Ploenes T, Herold T, Kalbourtzis S, Bankfalvi A, Sucker A, Kimmig R, Aigner C, Hegedus B. HDAC Inhibition Induces Cell Cycle Arrest and Mesenchymal-Epithelial Transition in a Novel Pleural-Effusion Derived Uterine Carcinosarcoma Cell Line. Pathology & Oncology Research 2021, 27: 636088. PMID: 34257602, PMCID: PMC8262245, DOI: 10.3389/pore.2021.636088.Peer-Reviewed Original ResearchMeSH KeywordsAntineoplastic Combined Chemotherapy ProtocolsBiomarkers, TumorCarcinosarcomaCell Cycle CheckpointsCisplatinEpithelial-Mesenchymal TransitionFemaleGene Expression Regulation, NeoplasticHistone DeacetylasesHumansMiddle AgedMutationPaclitaxelPhthalazinesPiperazinesPleural Effusion, MalignantPrognosisPyrazolesQuinolinesTumor Cells, CulturedUterine NeoplasmsVorinostatConceptsEpithelial-mesenchymal transitionUterine carcinosarcomaPleural effusionMesenchymal-epithelial transitionCell linesPatient-derived preclinical modelsMalignant pleural effusionMetastatic tumor lesionsVimentin-positive tumorsE-cadherinCarcinosarcoma cell lineInduces cell cycle arrestHistone deacetylase inhibitionFirst-line chemotherapeuticsΒ-catenin expressionE-cadherin expressionPSmad2 expressionCell cycle analysisPositive tumorsAggressive malignancyMetastatic tumorsDisease progressionCell cycle arrestNovel therapiesPreclinical models
2020
CSIG-08. TARGETING ION TRANSPORT-REGULATORY KINASES AS A NOVEL TREATMENT FOR GLIOBLASTOMA
Schiapparelli P, Meade P, Miranda-Herrera P, Bechtle A, Issacs F, Levchenko A, Rinehart J, Quinones-Hinojosa A. CSIG-08. TARGETING ION TRANSPORT-REGULATORY KINASES AS A NOVEL TREATMENT FOR GLIOBLASTOMA. Neuro-Oncology 2020, 22: ii29-ii29. PMCID: PMC7650317, DOI: 10.1093/neuonc/noaa215.120.Peer-Reviewed Original ResearchOxidative stress-responsive kinase 1Small molecule inhibitorsGBM cellsMolecule inhibitorsAggressive primary brain tumorCell migrationComplete surgical resectionMaximal safe resectionPrimary brain tumorsAdjacent brain parenchymaCell proliferationOrthotopic murine modelPatient-derived GBM cell linesDose-dependent reductionImportant therapeutic componentGBM cell linesCell cycle analysisGBM cell migrationSurgical resectionSafe resectionBrain parenchymaCell infiltrationCell cycle arrestMurine modelRadiation therapyThe palette of techniques for cell cycle analysis
Eastman AE, Guo S. The palette of techniques for cell cycle analysis. FEBS Letters 2020, 594: 2084-2098. PMID: 32441778, PMCID: PMC9261528, DOI: 10.1002/1873-3468.13842.Peer-Reviewed Original ResearchCell cycleCell cycle analysisCell fate specificationCell division cycleCell cycle speedSingle-cell eraSingle-cell resolutionCell cycle progressionCell cycle dynamicsMulticellular organismsFate specificationCell cycle heterogeneityGenomic fidelityDivision cycleBiochemical machineryTissue homeostasisCycle progressionCellular growthCell cycle measurementsCycle analysisPalette of techniquesGenerational periodCycle dynamicsCentral roleCell number
2016
Dual CCNE1/PIK3CA targeting is synergistic in CCNE1-amplified/PIK3CA-mutated uterine serous carcinomas in vitro and in vivo
Cocco E, Lopez S, Black J, Bellone S, Bonazzoli E, Predolini F, Ferrari F, Schwab CL, Menderes G, Zammataro L, Buza N, Hui P, Wong S, Zhao S, Bai Y, Rimm DL, Ratner E, Litkouhi B, Silasi DA, Azodi M, Schwartz PE, Santin AD. Dual CCNE1/PIK3CA targeting is synergistic in CCNE1-amplified/PIK3CA-mutated uterine serous carcinomas in vitro and in vivo. British Journal Of Cancer 2016, 115: 303-311. PMID: 27351214, PMCID: PMC4973158, DOI: 10.1038/bjc.2016.198.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntineoplastic AgentsCell Line, TumorClass I Phosphatidylinositol 3-KinasesCyclin EDNA Copy Number VariationsFemaleGene Knockdown TechniquesHeterograftsHumansIn Situ Hybridization, FluorescenceIn Vitro TechniquesMiceMutationOncogene ProteinsPhosphatidylinositol 3-KinasesRNA, MessengerTissue Array AnalysisUterine NeoplasmsConceptsUterine serous carcinomaSerous carcinomaTumor growthCyclin E1 (CCNE1) gene amplificationRecurrent uterine serous carcinomaPrimary USC cell linesNovel therapeutic optionsSingle-agent treatmentIdeal therapeutic targetUSC cell linesCyclin E1 expressionUSC patientsUSC xenograftsInhibited cell growthCell cycle analysisAggressive variantTherapeutic optionsCCNE1 amplificationEndometrial tumorsCYC065Therapeutic targetClinical optionPIK3CA driver mutationsDriver mutationsXenografts
2012
Olive Oil Polyphenols Differentially Inhibit Smooth Muscle Cell Proliferation through a G1/S Cell Cycle Block Regulated by ERK1/2
Abe R, Beckett J, Abe R, Nixon A, Rochier A, Yamashita N, Sumpio B. Olive Oil Polyphenols Differentially Inhibit Smooth Muscle Cell Proliferation through a G1/S Cell Cycle Block Regulated by ERK1/2. International Journal Of Angiology 2012, 21: 069-076. PMID: 23730132, PMCID: PMC3444010, DOI: 10.1055/s-0032-1315630.Peer-Reviewed Original ResearchSmooth muscle cell proliferationMuscle cell proliferationCell cycle blockOlive oil polyphenolsCell cycle analysisDay 1SMC proliferationG1/S cell cycle blockCycle blockOil polyphenolsRisk of atherosclerosisCell proliferationOLE groupControl groupFlow cytometryVascular SMCsWestern blottingDays of exposureS phaseCycle analysisERK1/2 activationNumber of cellsCell populationsOlive oilG1 phaseP190B RhoGAP Regulates Chromosome Segregation in Cancer Cells
Hwang M, Peddibhotla S, McHenry P, Chang P, Yochum Z, Park K, Sears J, Vargo-Gogola T. P190B RhoGAP Regulates Chromosome Segregation in Cancer Cells. Cancers 2012, 4: 475-489. PMID: 22582143, PMCID: PMC3348653, DOI: 10.3390/cancers4020475.Peer-Reviewed Original ResearchChromosome segregationMicrotubule-kinetochore attachmentsDisruption of mitosisRegulator of mitosisDeficient MCF-7 cellsP190B Rho GTPaseCancer cellsMitotic defectsRho proteinsRho GTPasesRac GTPaseRho GTPaseRac activityKnockdown cellsCell cycle analysisDeficient cellsChemical inhibitionNovel roleMitosisP190BS transitionS phaseHeLa cellsAltered expressionMCF-7 cellsRadio-Sensitization of Human Leukaemic MOLT-4 Cells by DNA-Dependent Protein Kinase Inhibitor, NU7026
Tichý A, Novotná E, Ďurišová K, Šalovská B, Sedlaříková R, Pejchal J, Zárybnická L, Vávrová J, Šinkorová Z, Řezáčová M. Radio-Sensitization of Human Leukaemic MOLT-4 Cells by DNA-Dependent Protein Kinase Inhibitor, NU7026. Acta Medica 2012, 55: 66-73. PMID: 23101268, DOI: 10.14712/18059694.2015.57.Peer-Reviewed Original ResearchConceptsMOLT-4 cellsDNA repairDNA-dependent protein kinase inhibitorDNA-dependent protein kinaseDNA damage responsePost-translational modificationsCheckpoint kinase 2Protein kinase inhibitorsG2 phase arrestInduction of apoptosisATM kinaseDamage responseProtein kinaseKinase 2Cell cycle analysisNU7026Specific inhibitorCellular mechanismsPronounced apoptosisApoptosisKinase inhibitorsAmount of cellsCellsCycle analysisInhibitors
2011
Olive Oil Polyphenol Oleuropein Inhibits Smooth Muscle Cell Proliferation
Abe R, Beckett J, Abe R, Nixon A, Rochier A, Yamashita N, Sumpio B. Olive Oil Polyphenol Oleuropein Inhibits Smooth Muscle Cell Proliferation. European Journal Of Vascular And Endovascular Surgery 2011, 41: 814-820. PMID: 21333557, DOI: 10.1016/j.ejvs.2010.12.021.Peer-Reviewed Original ResearchConceptsSmooth muscle cell proliferationMuscle cell proliferationCell cycle analysisSMC proliferationCell proliferationInhibits Smooth Muscle Cell ProliferationVascular smooth muscle cell proliferationCoronary artery diseaseNon-treated groupExtracellular signal-regulated kinase 1/2 activationCardiovascular mortalityArtery diseaseKinase 1/2 activationCell cycle blockG1-S phaseMediterranean dietDay 1Oleuropein treatmentGrowth of SMCsG1 phase regulatorsFlow cytometryVascular SMCsPresence of oleuropeinBeneficial effectsCycle analysis
2007
Multiple Defects of Both Primitive and Definitive Erythrocytes in EKLF-Deficient Mice.
Pilon A, Beaupre J, Bieker J, Gallagher P, Bodine D. Multiple Defects of Both Primitive and Definitive Erythrocytes in EKLF-Deficient Mice. Blood 2007, 110: 1234. DOI: 10.1182/blood.v110.11.1234.1234.Peer-Reviewed Original ResearchIngenuity Pathway AnalysisFL cellsSevere anemiaFetal liver cellsG0/G1Cell cycle analysisColony-forming assaysLevels of mRNADay 14Cytometric analysisAbsolute numberLiver cellsCell cycle progressionComparable reductionMiceDifferentiation blockOrthochromatic normoblastsNormoblastsKrüppel-like factorAnemiaTranscription factorsApoptosisOsmotic fragility assaysPathway analysisWT levelsActivation of the PTEN/mTOR/STAT3 pathway in breast cancer stem-like cells is required for viability and maintenance
Zhou J, Wulfkuhle J, Zhang H, Gu P, Yang Y, Deng J, Margolick JB, Liotta LA, Petricoin E, Zhang Y. Activation of the PTEN/mTOR/STAT3 pathway in breast cancer stem-like cells is required for viability and maintenance. Proceedings Of The National Academy Of Sciences Of The United States Of America 2007, 104: 16158-16163. PMID: 17911267, PMCID: PMC2042178, DOI: 10.1073/pnas.0702596104.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBreast NeoplasmsCell CycleCell Line, TumorCell SurvivalFemaleHumansMiceMice, NudeModels, BiologicalNeoplasm TransplantationNeoplastic Stem CellsOligonucleotide Array Sequence AnalysisProtein KinasesPTEN PhosphohydrolaseSignal TransductionSTAT3 Transcription FactorTOR Serine-Threonine KinasesConceptsCancer stem-like cellsStem-like cellsNon-SP cellsSP cellsATP-binding cassette (ABC) transportersTarget of rapamycinActivator of transcriptionCDNA microarray analysisPathway-specific inhibitorsSTAT3 pathwayVivo tumorigenicity assaysProtein microarray technologyRare cell populationsCytometry cell cycle analysisSide population cellsColony formation abilityMTOR/STAT3 pathwayCell maintenanceSignal transductionBreast cancer stem-like cellsGene knockdownNegative regulatorCassette transportersMicroarray analysisCell cycle analysis
2005
Resveratrol inhibits vascular smooth muscle cell proliferation and induces apoptosis
Poussier B, Cordova AC, Becquemin JP, Sumpio BE. Resveratrol inhibits vascular smooth muscle cell proliferation and induces apoptosis. Journal Of Vascular Surgery 2005, 42: 1190-1190.e14. PMID: 16376213, DOI: 10.1016/j.jvs.2005.08.014.Peer-Reviewed Original ResearchMeSH KeywordsAngiogenesis InhibitorsAnimalsAnimals, NewbornAntioxidantsAorta, ThoracicApoptosisBlotting, WesternCattleCell ProliferationCells, CulturedDNADose-Response Relationship, DrugFlow CytometryIn Situ Nick-End LabelingIn Vitro TechniquesMuscle, Smooth, VascularProliferating Cell Nuclear AntigenResveratrolStilbenesConceptsSmooth muscle cell proliferationMuscle cell proliferationVascular smooth muscle cell proliferationDose-dependent mannerSMC proliferationCell proliferationG1-S phaseBeneficial effectsFrench paradoxDose-dependent apoptotic effectTerminal deoxynucleotidyl transferase-mediated dUTP-biotin nickTransferase-mediated dUTP-biotin nickCardiovascular death ratesRed wine intakeRed wine consumptionEffects of resveratrolAortic SMC proliferationEnd labeling stainingVascular SMC proliferationDUTP-biotin nickMajor polyphenol componentHealthy life styleCell cycle analysisCardiovascular mortalityFlow-activated cell sorting
1995
Induction of p53 in mouse cells decreases mutagenesis by UV radiation
Yuan J, Yeasky T, Havre P, Glazer P. Induction of p53 in mouse cells decreases mutagenesis by UV radiation. Carcinogenesis 1995, 16: 2295-2300. PMID: 7586125, DOI: 10.1093/carcin/16.10.2295.Peer-Reviewed Original ResearchConceptsInduction of p53Cell cycle blockCell linesCycle blockRole of p53Cell cycle analysisInvolvement of p53Lambda phage shuttle vectorWestern blotChromosomal damageClonogenic survivalNucleotide excision repairUV-induced mutationsCellular DNA damageP53 alleleRecent evidenceP53Recoverable lambda phage shuttle vectorFibroblast cell lineMutation reporter geneUV-induced lesionsG1 phaseP53 activityMouse fibroblast cell lineReporter gene
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