In vitro selection of an RNA sequence that interacts with high affinity with thymidylate synthase
Lin X, Mizunuma N, Chen T, Copur SM, Maley GF, Liu J, Maley F, Chu E. In vitro selection of an RNA sequence that interacts with high affinity with thymidylate synthase. Nucleic Acids Research 2000, 28: 4266-4274. PMID: 11058126, PMCID: PMC113123, DOI: 10.1093/nar/28.21.4266.Peer-Reviewed Original ResearchMeSH KeywordsBase SequenceBlotting, WesternColonic NeoplasmsGenes, ReporterHumansMutationNuclease Protection AssaysNucleic Acid ConformationPlasmidsPrecipitin TestsProtein BindingRecombinant Fusion ProteinsReverse Transcriptase Polymerase Chain ReactionRNARNA, MessengerRNA-Binding ProteinsSubstrate SpecificityThymidylate SynthaseTransfectionTumor Cells, CulturedConceptsH630 cellsTS proteinLuciferase reporter constructsRNA sequencesRT-PCRSignificant increaseInduced expressionThymidylate synthaseVivo transfection experimentsCellsSingle RNA moleculesCis-acting elementsHigh-affinity RNAStem-loop structureSecondary structure analysisNovel RNA sequencesDirect bindingHigh affinityReporter constructsTransfection experimentsImmunoprecipitation methodRepressive effectRNA libraries
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