2013
Site-specific Genome Editing in PBMCs With PLGA Nanoparticle-delivered PNAs Confers HIV-1 Resistance in Humanized Mice
Schleifman EB, McNeer NA, Jackson A, Yamtich J, Brehm MA, Shultz LD, Greiner DL, Kumar P, Saltzman WM, Glazer PM. Site-specific Genome Editing in PBMCs With PLGA Nanoparticle-delivered PNAs Confers HIV-1 Resistance in Humanized Mice. Molecular Therapy - Nucleic Acids 2013, 2: e135. PMID: 24253260, PMCID: PMC3889188, DOI: 10.1038/mtna.2013.59.Peer-Reviewed Original ResearchPeripheral blood mononuclear cellsHuman peripheral blood mononuclear cellsHIV-1 resistanceHumanized miceCCR5 geneMolecular Therapy-Nucleic Acids (2013) 2Plasma viral RNA loadNOD-scid ILR5-tropic strainsBlood mononuclear cellsViral RNA loadWeeks posttransplantationControl miceMononuclear cellsRNA loadT cellsHIV-1Biodegradable poly (lactide-co-glycolide) (PLGA) nanoparticlesCCR2 geneSingle treatmentMiceCCR5PLGA NPsPeptide nucleic acidPLGA nanoparticles
2008
T Cell-Specific siRNA Delivery Suppresses HIV-1 Infection in Humanized Mice
Kumar P, Ban HS, Kim SS, Wu H, Pearson T, Greiner DL, Laouar A, Yao J, Haridas V, Habiro K, Yang YG, Jeong JH, Lee KY, Kim YH, Kim SW, Peipp M, Fey GH, Manjunath N, Shultz LD, Lee SK, Shankar P. T Cell-Specific siRNA Delivery Suppresses HIV-1 Infection in Humanized Mice. Cell 2008, 134: 577-586. PMID: 18691745, PMCID: PMC2943428, DOI: 10.1016/j.cell.2008.06.034.Peer-Reviewed Original ResearchConceptsHIV infectionAntiviral siRNAsT cellsAnimal modelsCD4 T-cell countCD4 T-cell lossPeripheral blood mononuclear cellsSuppress HIV-1 infectionHu-HSC miceHu-PBL miceT-cell countsT cell lossHIV-1 infectionBlood mononuclear cellsNaive T cellsPreclinical animal modelsSuitable animal modelHumanized miceInfected miceMononuclear cellsSuppress viremiaCell countCell lossTherapeutic potentialHematopoietic stem cells
2000
Ethyleneglycol-Bis-(β-Aminoethylether)Tetraacetate as a Blood Anticoagulant: Preservation of Antigen-Presenting Cell Function and Antigen-Specific Proliferative Response of Peripheral Blood Mononuclear Cells from Stored Blood
Kumar P, Satchidanandam V. Ethyleneglycol-Bis-(β-Aminoethylether)Tetraacetate as a Blood Anticoagulant: Preservation of Antigen-Presenting Cell Function and Antigen-Specific Proliferative Response of Peripheral Blood Mononuclear Cells from Stored Blood. MSphere 2000, 7: 578-583. PMID: 10882655, PMCID: PMC95917, DOI: 10.1128/cdli.7.4.578-583.2000.Peer-Reviewed Original ResearchConceptsPeripheral blood mononuclear cellsAntigen-specific responsesBlood mononuclear cellsJapanese encephalitis virusLymphoproliferative responsesMononuclear cellsT cellsFresh peripheral blood mononuclear cellsAdherent antigen-presenting cellsAntigen-Presenting Cell FunctionAntigen-specific proliferative responsesAntigen-specific T cell linesAntigen-specific lymphoproliferative responsesEffector T cellsAntigen-presenting cellsAnticoagulant of choiceT cell linesJEV patientsLymphocyte proliferationAntigen stimulationInterleukin-2Proliferative responseStored bloodStimulation assaysEncephalitis virus