Genomics and Bioinformatics Core
The Genomics Core provides high throughput sequencing services starting with experimental design, quality control of user materials, making sequencing library, generating sequencing results, and demultiplexing indexed samples providing raw sequencing data (FASTQ files) to the users. We have transitioned to a newer and more versatile sequencer, DNBSEQ-G400 from Complete Genomics Inc. Besides the standard sequencing services, this transition enables us to offer researchers a novel tool to explore spatial biology with subcellular resolution, which no other platform can achieve. Stereo-seq captures the RNA information in-situ within the tissue on the chip and through spatial coordination barcoding the cellular spatial location of the RNA can be restored subsequently. This advanced approach enables us to identify every cell and its location in the tissues.
In addition to the standard sequencing services, as continued core development, we have extended the Genomics Core service offerings into the multiomics field. The PhenoCycler-Fusion system is the fastest spatial biology solution from Akoya Bioscience Inc. The system allows unbiased spatial phenotyping of tissues, accounting for every single cell across the whole slide. We offer automated cycling and iterative imaging of 100+ biomarkers using this system on multiple tissue types, such as brain, liver, kidney, skin, both normal and diseased phenotypes. This unique proteomics technology can be used with both FFPE and/or fresh frozen tissues.
For Sample Submission:
- Submit the YSCC Genomics Core Sample Information Form
- Complete the Request for Services form. This form must provide full COA and PI approval signature. Send completed form to mei.zhong@yale.edu
- Current fiscal year pricing is listed on the Request for Services Form.
Contact:
Mei Zhong Ph.D.
Technical Director, Genomics Core of Yale Stem Cell Center
Yale University School of Medicine
10 Amistad Street, Room 201F
New Haven, CT 06520
Phone: (203) 737-6203
Email: mei.zhong@yale.edu
Location:
10 Amistad Street, Room 203
New Haven, CT 06520