2004
Roles of the Proline-rich Domain in SLP-76 Subcellular Localization and T Cell Function* [boxs]
Singer AL, Bunnell SC, Obstfeld AE, Jordan MS, Wu JN, Myung PS, Samelson LE, Koretzky GA. Roles of the Proline-rich Domain in SLP-76 Subcellular Localization and T Cell Function* [boxs]. Journal Of Biological Chemistry 2004, 279: 15481-15490. PMID: 14722089, DOI: 10.1074/jbc.m313339200.Peer-Reviewed Original ResearchMeSH KeywordsAdaptor Proteins, Signal TransducingAlanineAntigens, CDAntigens, Differentiation, T-LymphocyteArginineBlotting, WesternCalciumCell LineCell LineageFlow CytometryGene DeletionGenes, DominantHematopoietic Stem CellsHumansJurkat CellsLectins, C-TypeLuciferasesLymphocyte ActivationLysineMembrane MicrodomainsModels, BiologicalMutationPhosphoproteinsPlasmidsPrecipitin TestsProlineProtein Structure, TertiarySignal TransductionSrc Homology DomainsSubcellular FractionsTime FactorsT-LymphocytesTransfectionConceptsSLP-76P1 domainSLP-76 functionProline-rich domainProline-rich regionDomain deletion mutantMultiple hematopoietic lineagesLeukocyte-specific phosphoproteinSrc homologyDeletion mutantsSignal transductionSubcellular localizationHematopoietic lineagesFunctional roleProtein fragmentsT cell receptorMolecular scaffoldsCell functionCell receptorLocalizationComplementary approachesDomainT cell functionMolecular associationDirect evidence
2003
Inhibition of T cell activation through interruption of adaptor protein associations
Singer A, Bunnell S, Obstfeld A, Jordan M, Wu J, Myung P, Samelson L, Koretzky G. Inhibition of T cell activation through interruption of adaptor protein associations. Journal Of Surgical Research 2003, 114: 271. DOI: 10.1016/j.jss.2003.08.227.Peer-Reviewed Original ResearchSLP-76NF-ATSLP-76 functionTCR engagementT cell receptorMultiple hematopoietic lineagesLive confocal microscopyJurkat T cellsAdaptor LATInducible associationSecond messenger pathwaysBinding partnerComplex assemblyProtein associationBiochemical signalsHematopoietic lineagesAdaptor moleculeFusion proteinImmune synapseTCR stimulationJurkat cellsMessenger pathwaysCell membraneT cell activationBinding fragment
2001
Differential Requirement for SLP-76 Domains in T Cell Development and Function
Myung P, Derimanov G, Jordan M, Punt J, Liu Q, Judd B, Meyers E, Sigmund C, Freedman B, Koretzky G. Differential Requirement for SLP-76 Domains in T Cell Development and Function. Immunity 2001, 15: 1011-1026. PMID: 11754821, DOI: 10.1016/s1074-7613(01)00253-9.Peer-Reviewed Original ResearchMeSH KeywordsAdaptor Proteins, Signal TransducingAmino Acid MotifsAmino Acid SubstitutionAnimalsBinding SitesCalcium SignalingCarrier ProteinsCD3 ComplexCell DifferentiationClonal DeletionImmunophenotypingMembrane ProteinsMiceMice, Inbred C57BLMice, KnockoutMice, TransgenicMutation, MissensePhosphoproteinsProtein Structure, TertiaryReceptors, Antigen, T-CellRecombinant Fusion ProteinsSequence DeletionSignal TransductionSpleenSrc Homology DomainsStructure-Activity RelationshipThymus GlandT-LymphocytesConceptsSLP-76T cell developmentCell developmentDifferential requirementSLP-76 functionT cell receptor signalingCell receptor signalingAdaptor proteinMolecular mechanismsNull backgroundExamination of miceReceptor signalingCell functionPeripheral T cellsNew insightsTransgenic miceStructural requirementsSpecific domainsT cell functionT cellsCellsDomainMutantsThymocytesSignaling
2000
Hematopoietic reconstitution of SLP-76 corrects hemostasis and platelet signaling through αIIbβ3 and collagen receptors
Judd B, Myung P, Leng L, Obergfell A, Pear W, Shattil S, Koretzky G. Hematopoietic reconstitution of SLP-76 corrects hemostasis and platelet signaling through αIIbβ3 and collagen receptors. Proceedings Of The National Academy Of Sciences Of The United States Of America 2000, 97: 12056-12061. PMID: 11050236, PMCID: PMC17293, DOI: 10.1073/pnas.97.22.12056.Peer-Reviewed Original ResearchConceptsSLP-76Alpha IIbAdapter protein SLP-76SLP-76 functionSLP-76-deficient miceRapid tyrosine phosphorylationSLP-76-deficient plateletsTyrosine phosphorylated proteinsCollagen receptorCollagen receptor signalingT cell developmentPlatelet fibrinogen receptorPhosphorylated proteinsActin rearrangementCell peripheryLamellipodial extensionTyrosine phosphorylationCell developmentMurine plateletsRetroviral transductionReceptor signalingBind fibrinogenHematopoietic cellsFibrinogen receptorPlatelet-bound fibrinogen