2008
Local and Distant Elements Regulate Tissue-Specific Expression of ANK-1 Gene Transcripts
Owen A, Laflamme K, Pilon A, Garrett L, Gallagher P, Bodine D. Local and Distant Elements Regulate Tissue-Specific Expression of ANK-1 Gene Transcripts. Blood 2008, 112: 2432. DOI: 10.1182/blood.v112.11.2432.2432.Peer-Reviewed Original ResearchNon-erythroid cellsHypersensitive sitesTransient transfection assaysConsensus sequenceChromatin loopsGATA-1NF-E2Promoter sequencesKb regionHistone acetylationErythroid transcription factor NF-E2Transfection assaysFirst exonDNase IErythroid cellsDevelopmental stage-specific patternsLocal chromatin architectureProtein-coding genesChromatin conformation captureANK-1 geneRNA Pol IITranscription initiation complexErythroid K562 cellsDNase hypersensitive sitesGATA-1 binding
2006
Defects in E2F1/2 Expression Are Associated with Abnormalities in Cell Cycle and Differentiation in EKLF-Deficient Erythroid Cells.
Pilon A, Arcasoy M, Vayda S, Dressman H, Bieker J, Bodine D, Gallagher P. Defects in E2F1/2 Expression Are Associated with Abnormalities in Cell Cycle and Differentiation in EKLF-Deficient Erythroid Cells. Blood 2006, 108: 84. DOI: 10.1182/blood.v108.11.84.84.Peer-Reviewed Original ResearchHypersensitive sitesErythroid cellsMicroarray analysisCell cycleNumerous genesMature erythroblastsGel mobility shift assaysMicroarray dataFL cellsTranscription factor EKLFCell cycle controlMobility shift assaysΒ-globin expressionIngenuity Pathway AnalysisImmature erythroid cellsPrevious microarray analysisPrevious microarray dataE2F proteinsDefinitive erythropoiesisDNA replicationTranscription factorsColony forming assaysEKLFShift assaysTarget genesAlterations in Expression and Chromatin Configuration of the Alpha Hemoglobin-Stabilizing Protein Gene in Erythroid Krüppel-Like Factor-Deficient Mice
Pilon AM, Nilson DG, Zhou D, Sangerman J, Townes TM, Bodine DM, Gallagher PG. Alterations in Expression and Chromatin Configuration of the Alpha Hemoglobin-Stabilizing Protein Gene in Erythroid Krüppel-Like Factor-Deficient Mice. Molecular And Cellular Biology 2006, 26: 4368-4377. PMID: 16705186, PMCID: PMC1489081, DOI: 10.1128/mcb.02216-05.Peer-Reviewed Original ResearchConceptsErythroid Krüppel-like factorAlpha-hemoglobin-stabilizing proteinWild-type chromatinAHSP promoterCACCC siteBeta-globin gene transcriptionDNase I hypersensitive sitesLocal chromatin structureZinc finger proteinBeta-globin promoterKrüppel-like factorMobility shift assaysBeta-globin geneErythroid genesCACCC sequenceChromatin modulatorsFinger proteinChromatin structureSubtractive hybridizationChromatin statusCACCC boxTranscription factorsProtein geneChromatin configurationHypersensitive sites
2004
Multiple Defects in Erythroid Gene Expression in Erythroid Krupple-Like Factor (EKLF) Target Genes in EKLF-Deficient Mice.
Gallagher P, Pilon A, Arcasoy M, Bodine D. Multiple Defects in Erythroid Gene Expression in Erythroid Krupple-Like Factor (EKLF) Target Genes in EKLF-Deficient Mice. Blood 2004, 104: 1602. DOI: 10.1182/blood.v104.11.1602.1602.Peer-Reviewed Original ResearchDNaseI hypersensitive sitesSubtractive hybridizationHypersensitive sitesΒ-globin geneHistone H3Transcription factorsTarget genesFetal liver cellsΒ-spectrinGene expressionΒ-globinErythroid gene expressionMouse Genome 430 2.0 ArrayAffymetrix GeneChip Mouse Genome 430 2.0 ArrayFactor target genesAHSP gene expressionChromatin immunoprecipitation analysisBand 3Mature erythroid progenitorsAHSP promoterRBC membrane proteinsErythroid genesChromatin upstreamChromatin modulatorsRegulated genesChromatin Remodeling of the Mouse AHSP Gene Requires EKLF.
Pilon A, Wong C, Garrett-Beal L, Weiss M, Gallagher P, Bodine D. Chromatin Remodeling of the Mouse AHSP Gene Requires EKLF. Blood 2004, 104: 375. DOI: 10.1182/blood.v104.11.375.375.Peer-Reviewed Original ResearchAlpha-Hemoglobin Stabilizing ProteinDNase I hypersensitive sitesAHSP geneAHSP gene expressionHypersensitive sitesWild typeΓ-globin mRNAFetal liver cellsHistone H3Human γ-globin genesGene expressionStrong DNase I hypersensitive siteIntron 1Wild-type chromatinChromatin Remodeling ComplexErythroid-specific proteinΓ-globin genePutative promoter sequencesTranscription factor EKLFExon 1Non-coding exonsΑ-globin mRNAPosition-independent expressionMRNA initiation siteRNase protection analysisSequences Downstream of the Erythroid Promoter Are Required for High Level Expression of the Human α-Spectrin Gene*
Wong EY, Lin J, Forget BG, Bodine DM, Gallagher PG. Sequences Downstream of the Erythroid Promoter Are Required for High Level Expression of the Human α-Spectrin Gene*. Journal Of Biological Chemistry 2004, 279: 55024-55033. PMID: 15456760, DOI: 10.1074/jbc.m408886200.Peer-Reviewed Original ResearchMeSH KeywordsBase SequenceBinding SitesCell DifferentiationCell MembraneCell NucleusChromatin ImmunoprecipitationCREB-Binding ProteinDeoxyribonuclease IDNADNA PrimersDNA-Binding ProteinsDNA, ComplementaryErythrocytesErythroid-Specific DNA-Binding FactorsEthidiumExonsGATA1 Transcription FactorGenes, ReporterHeLa CellsHumansImmunoprecipitationIntronsK562 CellsLuciferasesModels, GeneticMolecular Sequence DataMutationNuclear ProteinsPlasmidsPromoter Regions, GeneticSpectrinTemperatureTrans-ActivatorsTranscription FactorsTransfectionConceptsErythroid-specific expressionAlpha-spectrin geneGATA-1 sitesCore promoterDNase I hypersensitive sitesElectrophoretic mobility shift assaysChromatin immunoprecipitation assaysMobility shift assaysΑ-spectrin geneThymidine kinase promoterPositive regulatory elementHigh-level expressionGenomic orientationErythroid promoterGATA-1Membrane proteinsHypersensitive sitesImmunoprecipitation assaysRegulatory elementsSequence downstreamShift assaysErythroid differentiationTransfection assaysEnhancer activityReporter gene