2021
An IDH1-vitamin C crosstalk drives human erythroid development by inhibiting pro-oxidant mitochondrial metabolism
Gonzalez-Menendez P, Romano M, Yan H, Deshmukh R, Papoin J, Oburoglu L, Daumur M, Dumé AS, Phadke I, Mongellaz C, Qu X, Bories PN, Fontenay M, An X, Dardalhon V, Sitbon M, Zimmermann VS, Gallagher PG, Tardito S, Blanc L, Mohandas N, Taylor N, Kinet S. An IDH1-vitamin C crosstalk drives human erythroid development by inhibiting pro-oxidant mitochondrial metabolism. Cell Reports 2021, 34: 108723. PMID: 33535038, PMCID: PMC9169698, DOI: 10.1016/j.celrep.2021.108723.Peer-Reviewed Original ResearchConceptsIsocitrate dehydrogenase 1Oxidative phosphorylationMitochondrial metabolismReactive oxygen speciesHuman erythroid differentiationHuman erythroid developmentMitochondrial oxidative phosphorylationVitamin C homeostasisHSPC developmentIDH1 knockdownErythroid developmentStepwise differentiationErythroid differentiationLate-stage erythropoiesisTerminal stepCritical regulatorHematopoietic stemMitochondrial superoxideMitochondrial oxidationProgenitor cellsDehydrogenase 1Oxygen speciesCongenital dyserythropoietic anemiaCentral roleDyserythropoietic anemia
2017
Distinct roles for TET family proteins in regulating human erythropoiesis
Yan H, Wang Y, Qu X, Li J, Hale J, Huang Y, An C, Papoin J, Guo X, Chen L, Kang Q, Li W, Schulz VP, Gallagher PG, Hillyer CD, Mohandas N, An X. Distinct roles for TET family proteins in regulating human erythropoiesis. Blood 2017, 129: 2002-2012. PMID: 28167661, PMCID: PMC5383871, DOI: 10.1182/blood-2016-08-736587.Peer-Reviewed Original ResearchConceptsMyelodysplastic syndromeErythroid differentiationHuman erythropoiesisErythroid progenitorsHuman erythroid differentiationTET family proteinsDistinct rolesKnockdown of TET2Terminal erythroid differentiationHuman erythroid cellsTET2 gene mutationsFamily proteinsTranslocation (TET) familyTET2 knockdownKnockdown experimentsErythroid cellsBiological processesDevelopment defectsTET3TET3 expressionOrthochromatic erythroblastsImpaired differentiationHuman CD34KnockdownTET2