2020
A Static Self-Directed Method for Generating Brain Organoids from Human Embryonic Stem Cells.
Boisvert EM, Means RE, Michaud M, Thomson JJ, Madri JA, Katz SG. A Static Self-Directed Method for Generating Brain Organoids from Human Embryonic Stem Cells. Journal Of Visualized Experiments 2020 PMID: 32202516, PMCID: PMC7245934, DOI: 10.3791/60379.Peer-Reviewed Original ResearchConceptsEmbryonic stem cellsCell typesStem cellsIntrinsic developmental cuesHuman embryonic stem cellsHuman pluripotent stem cellsBrain organoidsBrain cell typesPluripotent stem cellsBasement membrane matrixMultiple cell typesDevelopmental cuesUse of organoidsExogenous growth factorsQuantitative reverse transcription polymerase chain reactionMultitude of diseasesHuman brain organoidsOrganoid growthSingle cellsReal-time quantitative reverse transcription polymerase chain reactionSpatial organizationOrganoidsGenetic disordersGrowth factorReverse transcription-polymerase chain reaction
2011
GSK-3β: a signaling pathway node modulating neural stem cell and endothelial cell interactions
Li Q, Michaud M, Canosa S, Kuo A, Madri JA. GSK-3β: a signaling pathway node modulating neural stem cell and endothelial cell interactions. Angiogenesis 2011, 14: 173-185. PMID: 21253820, DOI: 10.1007/s10456-011-9201-9.Peer-Reviewed Original ResearchMeSH KeywordsAminophenolsAnimalsBasic Helix-Loop-Helix Transcription FactorsBeta CateninBrainCell CommunicationCell DifferentiationCell MovementCell ProliferationEndothelial CellsEnzyme ActivationGlycogen Synthase Kinase 3Glycogen Synthase Kinase 3 betaHypoxia-Inducible Factor 1, alpha SubunitIntercellular Signaling Peptides and ProteinsMaleMaleimidesMiceMice, Inbred C57BLNeovascularization, PhysiologicNeural Stem CellsNeurogenesisPhosphorylationPhosphoserineReceptor Cross-TalkSignal TransductionSolubilitySpecies SpecificityConceptsNeural stem cellsNotch-1 expressionHIF-1αGSK-3βSDF-1III-tubulinStem cellsPremature infant populationMicrovascular endothelial cellsGSK-3β activationCD1 levelsEndothelial cell interactionsNeurogenic areasVascular proliferationInfant populationGSK-3β inhibitorTherapeutic potentialSVZ tissueGreater angiogenesisHIF-2αMouse strainsΒ-catenin participatesEndothelial cellsReciprocal modulation
2010
Angiogenesis, the Neurovascular Niche and Neuronal Reintegration After Injury
Lavik E, Madri J. Angiogenesis, the Neurovascular Niche and Neuronal Reintegration After Injury. 2010, 145-167. DOI: 10.1007/978-90-481-9495-7_7.Peer-Reviewed Original ResearchCell-matrix interactionsCentral nervous systemNeurogenic zonesCell biologyClinical improvementAffected individualsExtracellular matrixStem cellsCell proliferationNeuro-genesisNervous systemNeurodegenerative diseasesRepair processNicheTissue cultureSpinal cord injuryNeurovascular nicheComplete understandingDisease states
2006
Modeling the neurovascular niche: VEGF‐ and BDNF‐mediated cross‐talk between neural stem cells and endothelial cells: An in vitro study
Li Q, Ford MC, Lavik EB, Madri JA. Modeling the neurovascular niche: VEGF‐ and BDNF‐mediated cross‐talk between neural stem cells and endothelial cells: An in vitro study. Journal Of Neuroscience Research 2006, 84: 1656-1668. PMID: 17061253, DOI: 10.1002/jnr.21087.Peer-Reviewed Original ResearchMeSH KeywordsAnalysis of VarianceAnimalsAnimals, NewbornBrainBrain-Derived Neurotrophic FactorCell CommunicationCell ProliferationCells, CulturedCoculture TechniquesEndothelial CellsEnzyme-Linked Immunosorbent AssayGreen Fluorescent ProteinsMiceMice, Inbred C57BLMice, TransgenicMicroscopy, Electron, TransmissionModels, BiologicalNerve Tissue ProteinsNeuronsNitric OxidePlatelet Endothelial Cell Adhesion Molecule-1Stem CellsVascular Endothelial Growth Factor AConceptsBrain-derived neurotrophic factorBrain-derived endothelial cellsNeural stem cellsNeurovascular nicheTube formationResident neural stem cellsEndothelial cellsCell-derived soluble factorsVascular endothelial growth factorStem cellsNitric oxide scavengerEndothelial growth factorPaucity of dataExogenous NO donorNeurotrophic factorStem cell modulationVascular tube formationCell modulationENOS activationNO donorSoluble factorsGrowth factorNeuronal differentiationReciprocal modulationInductionPECAM‐1: a multi‐faceted regulator of megakaryocytopoiesis
Wu Y, Madri J. PECAM‐1: a multi‐faceted regulator of megakaryocytopoiesis. The FASEB Journal 2006, 20: a633-a633. DOI: 10.1096/fasebj.20.4.a633-c.Peer-Reviewed Original ResearchNull animalsMulti-faceted regulatorAltered localizationActin filament organizationProgenitor cellsDifferentiation of megakaryocytesCommitted progenitor cellsEndothelial cell monolayersQuiescent G0 phaseHematopoietic stem cellsSHP-2Progenitor populationsMarrow progenitor cellsSHP-1Filament organizationPECAM-1Mature megakaryocytesCell monolayersFGF-4Colony formation potentialStem cellsAbnormal megakaryocytopoiesisMegakaryocyte adhesionG0 phaseLSK population
1998
Immunofluorescence Characterization of Key Extracellular Matrix Proteins in Murine Bone Marrow In Situ
Nilsson S, Debatis M, Dooner M, Madri J, Quesenberry P, Becker P. Immunofluorescence Characterization of Key Extracellular Matrix Proteins in Murine Bone Marrow In Situ. Journal Of Histochemistry & Cytochemistry 1998, 46: 371-377. PMID: 9487119, DOI: 10.1177/002215549804600311.Peer-Reviewed Original ResearchConceptsExtracellular matrix proteinsMatrix proteinsKey extracellular matrix proteinsParticular extracellular matrix proteinsExtracellular matrix protein fibronectinStem cellsCentral marrow regionsMatrix protein fibronectinStem cell proliferationCollagen type IMurine bone marrowHemopoietic stem cellsProtein fibronectinBone marrow vesselsExtracellular matrixBone marrowMechanistic roleCell proliferationProteinMarrow microenvironmentImportant mechanistic roleMolecular interactionsImmunofluorescence labelingCollagen type IVType I