2021
Vulnerability of cholecystokinin-expressing GABAergic interneurons in the unilateral intrahippocampal kainate mouse model of temporal lobe epilepsy
Kang YJ, Clement EM, Park IH, Greenfield LJ, Smith BN, Lee SH. Vulnerability of cholecystokinin-expressing GABAergic interneurons in the unilateral intrahippocampal kainate mouse model of temporal lobe epilepsy. Experimental Neurology 2021, 342: 113724. PMID: 33915166, PMCID: PMC8192495, DOI: 10.1016/j.expneurol.2021.113724.Peer-Reviewed Original ResearchConceptsTemporal lobe epilepsyIntrahippocampal kainate mouse modelVentral CA1 regionEpileptic miceCA1 pyramidal cellsSclerotic hippocampusPyramidal cellsSham controlsSpontaneous seizuresLobe epilepsyCA1 regionMouse modelFrequency of IPSCsNetwork oscillationsRecurrent spontaneous seizuresNumber of boutonsHippocampal theta oscillationsIpsilateral hippocampusElectrical recordingsVentral hippocampusCA1 layerGABAergic interneuronsStratum pyramidaleDorsal hippocampusBehavioral comorbidities
2020
Deconstructing and reconstructing the human brain with regionally specified brain organoids
Xiang Y, Cakir B, Park IH. Deconstructing and reconstructing the human brain with regionally specified brain organoids. Seminars In Cell And Developmental Biology 2020, 111: 40-51. PMID: 32553582, DOI: 10.1016/j.semcdb.2020.05.023.Peer-Reviewed Original ResearchDysregulation of BRD4 Function Underlies the Functional Abnormalities of MeCP2 Mutant Neurons
Xiang Y, Tanaka Y, Patterson B, Hwang SM, Hysolli E, Cakir B, Kim KY, Wang W, Kang YJ, Clement EM, Zhong M, Lee SH, Cho YS, Patra P, Sullivan GJ, Weissman SM, Park IH. Dysregulation of BRD4 Function Underlies the Functional Abnormalities of MeCP2 Mutant Neurons. Molecular Cell 2020, 79: 84-98.e9. PMID: 32526163, PMCID: PMC7375197, DOI: 10.1016/j.molcel.2020.05.016.Peer-Reviewed Original ResearchConceptsMECP2 mutant neuronsEnhancer-promoter interactionsRett syndromeRTT-like phenotypesChromatin bindingMeCP2 functionMethyl-CpGAbnormal transcriptionRTT etiologyMutant neuronsBET inhibitorsPotential therapeutic opportunitiesMECP2 mutationsProtein 2Human brain organoidsFunctional phenotypeJQ1BRD4Therapeutic opportunitiesBrain organoidsFunction underliesMutationsPhenotypeHuman brain culturesCritical driver
2019
The critical role of persistent sodium current in hippocampal gamma oscillations
Kang YJ, Clement EM, Sumsky SL, Xiang Y, Park IH, Santaniello S, Greenfield LJ, Garcia-Rill E, Smith BN, Lee SH. The critical role of persistent sodium current in hippocampal gamma oscillations. Neuropharmacology 2019, 162: 107787. PMID: 31550457, PMCID: PMC6952064, DOI: 10.1016/j.neuropharm.2019.107787.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCA1 Region, HippocampalCalcium-Calmodulin-Dependent Protein Kinase Type 2Excitatory Amino Acid AntagonistsExcitatory Postsynaptic PotentialsGABAergic NeuronsGamma RhythmHippocampusInhibitory Postsynaptic PotentialsInterneuronsMiceOptogeneticsParvalbuminsPatch-Clamp TechniquesPhenytoinPyramidal CellsRiluzoleSodiumVoltage-Gated Sodium Channel BlockersConceptsParvalbumin-expressing basket cellsHippocampal gamma oscillationsCortical gamma oscillationsGABAergic interneuronsGamma oscillationsPyramidal cellsNon-inactivating sodium currentExcitatory cellsSodium currentWhole-cell patch-clamp recordingsNetwork oscillationsPatch-clamp recordingsPersistent sodium currentGamma network oscillationsAnticonvulsant efficacyGamma frequency rangeEpilepsy patientsBasket cellsCognitive impairmentAction potentialsSynaptic propertiesSynaptic interactionsOptogenetic stimulationElectrophysiological approachesCA1 network
2018
Generation and Fusion of Human Cortical and Medial Ganglionic Eminence Brain Organoids
Xiang Y, Yoshiaki T, Patterson B, Cakir B, Kim K, Cho YS, Park I. Generation and Fusion of Human Cortical and Medial Ganglionic Eminence Brain Organoids. Current Protocols In Stem Cell Biology 2018, 47 PMID: 30854156, PMCID: PMC6402040, DOI: 10.1002/cpsc.61.Peer-Reviewed Original ResearchMeSH KeywordsBrainHumansInterneuronsMedian EminenceNeurogenesisOrgan Culture TechniquesOrganoidsPluripotent Stem CellsConceptsMedial ganglionic eminenceBrain organoidsBrain regionsBrain developmentSpecific brain regionsDifferent brain regionsHuman CorticalHuman brain developmentCortical organoidsGanglionic eminenceNeurological disordersInterneuron migrationSpecific regionalizationOrganoid culturesOrganoidsBrain organoid culture
2017
Fusion of Regionally Specified hPSC-Derived Organoids Models Human Brain Development and Interneuron Migration
Xiang Y, Tanaka Y, Patterson B, Kang YJ, Govindaiah G, Roselaar N, Cakir B, Kim KY, Lombroso AP, Hwang SM, Zhong M, Stanley EG, Elefanty AG, Naegele JR, Lee SH, Weissman SM, Park IH. Fusion of Regionally Specified hPSC-Derived Organoids Models Human Brain Development and Interneuron Migration. Cell Stem Cell 2017, 21: 383-398.e7. PMID: 28757360, PMCID: PMC5720381, DOI: 10.1016/j.stem.2017.07.007.Peer-Reviewed Original ResearchConceptsHuman brain developmentChromatin accessibility dynamicsTransposase-accessible chromatinHigh-throughput sequencing analysisRegion-specific organoidsHuman pluripotent stem cellsRNA sequencing profilingHuman interneuron migrationPluripotent stem cellsRelated lineagesBrain developmentAccessibility dynamicsBulk assaysInterneuron migrationLineage relationshipsOrganoid techniquesSequencing profilingSequencing analysisFunctional neuronsOrganoid developmentStem cellsCortical organoidsOrganoidsBrain organoidsMGE