2024
Mechano-inhibition of endocytosis sensitizes cancer cells to Fas-induced Apoptosis
Kural M, Djakbarova U, Cakir B, Tanaka Y, Chan E, Arteaga Muniz V, Madraki Y, Qian H, Park J, Sewanan L, Park I, Niklason L, Kural C. Mechano-inhibition of endocytosis sensitizes cancer cells to Fas-induced Apoptosis. Cell Death & Disease 2024, 15: 440. PMID: 38909035, PMCID: PMC11193792, DOI: 10.1038/s41419-024-06822-3.Peer-Reviewed Original ResearchConceptsFas-induced apoptosisCell surface Fas expressionDeath receptor FasInhibition of endocytosisSurface Fas expressionPlasma membrane tensionCancer cell apoptosisEndocytosis dynamicsApoptotic signalingReceptor FasGlioblastoma cell growthFas expressionPlasma membraneCell growthEndocytosisXenograft mouse modelSoluble FasLCell apoptosisFasApoptosisRho-kinase inhibitorCancer cellsMembrane tensionNonmalignant cellsInduce tumor regression
2020
Implantation of the clinical‐grade human neural stem cell line, CTX0E03, rescues the behavioral and pathological deficits in the quinolinic acid‐lesioned rodent model of Huntington's disease
Yoon Y, Kim HS, Jeon I, Noh J, Park HJ, Lee S, Park I, Stevanato L, Hicks C, Corteling R, Barker RA, Sinden JD, Song J. Implantation of the clinical‐grade human neural stem cell line, CTX0E03, rescues the behavioral and pathological deficits in the quinolinic acid‐lesioned rodent model of Huntington's disease. Stem Cells 2020, 38: 936-947. PMID: 32374064, PMCID: PMC7496241, DOI: 10.1002/stem.3191.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell Line, TumorDisease Models, AnimalHumansHuntington DiseaseMiceNeoplasm GradingNeural Stem CellsQuinolinic AcidConceptsMedium spiny neuronsNeural stem cell lineHuntington's diseaseQuinolinic acid (QA) lesion rat modelChronic ischemic stroke patientsStriatal medium spiny neuronsCell linesImmortalized neural stem cell linesIschemic stroke patientsDisease-modifying therapiesSignals of efficacyGlial scar formationHost brain tissueHuman neural stem cell lineSignificant behavioral improvementAutosomal dominant neurodegenerative diseaseCTX0E03 cellsEndogenous neurogenesisBDNF expressionGABAergic neuronsHD patientsStroke patientsFluoro-GoldRetrograde labelSpiny neurons
2017
Bisulfite-independent analysis of CpG island methylation enables genome-scale stratification of single cells
Han L, Wu HJ, Zhu H, Kim KY, Marjani SL, Riester M, Euskirchen G, Zi X, Yang J, Han J, Snyder M, Park IH, Irizarry R, Weissman SM, Michor F, Fan R, Pan X. Bisulfite-independent analysis of CpG island methylation enables genome-scale stratification of single cells. Nucleic Acids Research 2017, 45: e77-e77. PMID: 28126923, PMCID: PMC5605247, DOI: 10.1093/nar/gkx026.Peer-Reviewed Original ResearchMeSH KeywordsCell LineCell Line, TumorChromosome MappingCpG IslandsDNA MethylationDNA Restriction EnzymesEpigenesis, GeneticFibroblastsGenetic VariationGenome, HumanHigh-Throughput Nucleotide SequencingHumansInduced Pluripotent Stem CellsK562 CellsLymphocytesPromoter Regions, GeneticSingle-Cell AnalysisConceptsSingle cellsMethylation-sensitive restriction enzyme digestionCpG methylation patternsDNA bisulfite sequencingInduced pluripotent stem cellsSingle-cell levelCpG island methylationPluripotent stem cellsHeterogeneous cell populationsMultiple displacement amplificationEpigenetic heterogeneityMethylation sequencingBisulfite sequencingENCODE dataMethylation patternsMethylation differencesMethylation profilesRestriction enzyme digestionIsland methylationIndividual cellsHematopoietic cellsStem cellsSmall populationSequencingEnzyme digestion
2015
Histone Deacetylases Positively Regulate Transcription through the Elongation Machinery
Greer CB, Tanaka Y, Kim YJ, Xie P, Zhang MQ, Park IH, Kim TH. Histone Deacetylases Positively Regulate Transcription through the Elongation Machinery. Cell Reports 2015, 13: 1444-1455. PMID: 26549458, PMCID: PMC4934896, DOI: 10.1016/j.celrep.2015.10.013.Peer-Reviewed Original ResearchMeSH KeywordsAcetylationCell Cycle ProteinsCell Line, TumorEpigenesis, GeneticHistone Deacetylase InhibitorsHistone DeacetylasesHSP90 Heat-Shock ProteinsHumansKineticsNuclear ProteinsProtein BindingProtein Processing, Post-TranslationalRNA Polymerase IITranscription Elongation, GeneticTranscription FactorsConceptsNegative elongation factorElongation factorEnhancer activityHeat shock protein 90 (HSP90) activityEnhancer RNA productionRNA polymerase IISame genomic siteElongation machinerySmall molecule inhibitorsGene bodiesTranscription elongationPolymerase IINascent transcriptionGenomic sitesIntergenic regionGene activationRNA productionEfficient elongationMolecule inhibitorsProtein 4TranscriptionPromoterHDACsEnhancerElongation
2012
The lesser known story of X chromosome reactivation
Hysolli E, Tanaka Y, Kim K, Jung Y, Park IH. The lesser known story of X chromosome reactivation. Cell Cycle 2012, 11: 229-235. PMID: 22234239, PMCID: PMC3293375, DOI: 10.4161/cc.11.2.18998.Peer-Reviewed Original ResearchConceptsX chromosome reactivationX-chromosome inactivationInner cell massActive histone marksGerm cell formationX inactivation signalHistone marksActive chromosomeCellular reprogrammingInactivation signalGene poolPluripotent cellsKey regulatorFemale cellsGene expressionGerm layersCell formationXY cellsCell massImportant mechanismNatural occurrenceCellsReprogrammingChromosomesGenes