2012
Reprogramming human somatic cells into induced pluripotent stem cells (iPSCs) using retroviral vector with GFP.
Kim KY, Hysolli E, Park IH. Reprogramming human somatic cells into induced pluripotent stem cells (iPSCs) using retroviral vector with GFP. Journal Of Visualized Experiments 2012 PMID: 22491226, PMCID: PMC3466658, DOI: 10.3791/3804.Peer-Reviewed Original ResearchConceptsHuman embryonic stem cellsInduced pluripotent stem cellsHuman somatic cellsHuman induced pluripotent stem cellsPluripotent stem cellsSomatic cellsIPSC coloniesStem cellsESC culture conditionsEmbryonic stem cellsPluripotency genesTranscription factorsRetroviral transgenesEctopic expressionGFP fluorescenceRetroviral vectorsHuman fibroblast cellsFibroblast cellsGFPCulture conditionsCellsAutologous cellsCellular sourceColoniesSurface markers
2009
Human iPS Cell Derivation/Reprogramming
Park I, Daley GQ. Human iPS Cell Derivation/Reprogramming. Current Protocols In Stem Cell Biology 2009, 8: 4a.1.1-4a.1.8. PMID: 19170021, DOI: 10.1002/9780470151808.sc04a01s8.Peer-Reviewed Original ResearchConceptsHES cellsHuman fibroblast cellsInduced pluripotent stem cellsFibroblast cellsHuman iPS cellsPluripotent stem cellsPluripotency genesTranscription factorsEmbryoid bodiesGerm layersIPS cellsStem cell culture conditionsCell coloniesCell culture conditionsStem cellsRetroviral vectorsCulture conditionsCellsReprogrammingGenesDifferentiationColoniesDissection microscope