2016
Single cell transcriptomics reveals unanticipated features of early hematopoietic precursors
Yang J, Tanaka Y, Seay M, Li Z, Jin J, Garmire LX, Zhu X, Taylor A, Li W, Euskirchen G, Halene S, Kluger Y, Snyder MP, Park IH, Pan X, Weissman SM. Single cell transcriptomics reveals unanticipated features of early hematopoietic precursors. Nucleic Acids Research 2016, 45: 1281-1296. PMID: 28003475, PMCID: PMC5388401, DOI: 10.1093/nar/gkw1214.Peer-Reviewed Original ResearchConceptsHematopoietic stem cellsPrecursor cellsInduction of anemiaInterferon response genesG2/M phaseEarly precursor cellsHomeostatic cellsStages of differentiationTranscription factorsSurface markersCell cycle progressionLong-term hematopoietic stem cellsSpecific augmentationAnemic miceMarked increaseEarly hematopoietic precursorsHematopoietic precursorsStem cellsCycle progressionM phaseSingle-cell transcriptomicsCellsCell differentiationHematopoietic stressLineage-specific transcription factors
2012
Overcoming reprogramming resistance of Fanconi anemia cells
Müller LU, Milsom MD, Harris CE, Vyas R, Brumme KM, Parmar K, Moreau LA, Schambach A, Park IH, London WB, Strait K, Schlaeger T, DeVine AL, Grassman E, D'Andrea A, Daley GQ, Williams DA. Overcoming reprogramming resistance of Fanconi anemia cells. Blood 2012, 119: 5449-5457. PMID: 22371882, PMCID: PMC3369681, DOI: 10.1182/blood-2012-02-408674.Peer-Reviewed Original ResearchConceptsFA cellsFA pathwayFA DNA repair pathwayFanconi anemiaDNA double-strand breaksFanconi anemia cellsStem cellsDNA repair pathwaysDouble-strand breaksDisease-specific iPSCsPluripotent stem cellsFuture translational applicationsGenomic integrityHuman primary cellsHematopoietic stem cellsHematopoietic differentiationChromosomal instabilityMolecular characterizationGene correctionTransgenic expressionDNA damageGenetic correctionHematopoietic cellsPrimary cellsPathway
2011
Evi-1 Regulates Myelopoiesis and Hematopoietic Stem Cell Development in Zebrafish and Human Pluripotent Stem Cells
Konantz M, Grauer M, Grzywna S, Brugman M, Kanz L, Park I, Daley G, Baum C, Lengerke C. Evi-1 Regulates Myelopoiesis and Hematopoietic Stem Cell Development in Zebrafish and Human Pluripotent Stem Cells. Blood 2011, 118: 1281. DOI: 10.1182/blood.v118.21.1281.1281.Peer-Reviewed Original ResearchHuman pluripotent stem cellsPluripotent stem cellsEvi-1Developmental hematopoiesisZebrafish embryosHematopoietic developmentHematopoietic stem cellsStem cellsPosterior lateral plate mesodermHematopoietic stem cell developmentPosterior blood islandHuman developmental hematopoiesisLateral plate mesodermStem cell developmentPre-mRNA splicingEvi-1 locusDays post fertilizationHematopoietic stem cell proliferationMurine hematopoietic cellsStem cell proliferationExpression of PUEmbryonic myelopoiesisEvi-1 expressionErythroid progenitor cellsPrimitive erythroid progenitor cellsGene-Correction Rescues Reprogramming of Fanconi Anemia Fibroblasts and Enables Hematopoietic Differentiation of FA Induced Pluripotent Stem Cells in Vitro and In Vivo
Mueller L, Milsom M, Harris C, Vyas R, Brumme K, Parmar K, Schambach A, Grassman E, Park I, Wendy L, Strait K, Schlaeger T, Devine A, D'Andrea A, Daley G, Williams D. Gene-Correction Rescues Reprogramming of Fanconi Anemia Fibroblasts and Enables Hematopoietic Differentiation of FA Induced Pluripotent Stem Cells in Vitro and In Vivo. Blood 2011, 118: 672. DOI: 10.1182/blood.v118.21.672.672.Peer-Reviewed Original ResearchFA cellsFA pathwayFanconi anemiaPluripotent stem cellsHematopoietic differentiationGenomic instabilityStem cellsIPSC linesDouble-strand DNA breaksHematopoietic cellsFANCD2 foci formationPatient iPSC linesHematopoietic differentiation potentialFanconi anemia fibroblastsTail-tip fibroblastsInduced pluripotent stem cellsDefective DNA repairGreen fluorescent proteinFatal bone marrow failureGenomic integritySignificant chromosomal imbalancesHematopoietic stem cellsComparative genomic hybridizationComplementation groupsDNA repair
2009
Hematopoietic Development From Human Induced Pluripotent Stem Cells.
Grauer M, Konantz M, Niebuhr N, Kanz L, Park I, Daley G, Lengerke C. Hematopoietic Development From Human Induced Pluripotent Stem Cells. Blood 2009, 114: 2530. DOI: 10.1182/blood.v114.22.2530.2530.Peer-Reviewed Original ResearchMouse embryonic stem cellsEmbryonic stem cellsInduced pluripotent stem cellsPluripotent stem cellsHuman embryonic stem cellsHematopoietic stem cellsHuman induced pluripotent stem cellsHuman iPS cellsIPS cellsCdx genesHematopoietic developmentBlood lineagesStem cellsBlood formationEmbryonic blood formationGenetic modificationHuman developmental hematopoiesisDifferentiated somatic cellsHuman pluripotent stem cell linesStem cell linesIrradiated adult micePluripotent stem cell lineDevelopmental hematopoiesisHematopoietic genesHox genesHematopoietic Development from Human Induced Pluripotent Stem Cells
Lengerke C, Grauer M, Niebuhr NI, Riedt T, Kanz L, Park I, Daley GQ. Hematopoietic Development from Human Induced Pluripotent Stem Cells. Annals Of The New York Academy Of Sciences 2009, 1176: 219-227. PMID: 19796250, PMCID: PMC2849804, DOI: 10.1111/j.1749-6632.2009.04606.x.Peer-Reviewed Original ResearchConceptsEmbryonic stem cellsMouse embryonic stem cellsPluripotent stem cellsHematopoietic developmentInduced pluripotent stem cellsHuman induced pluripotent stem cellsHematopoietic stem cellsStem cellsDifferentiated somatic cellsHuman embryonic stem cellsHuman hematopoietic developmentStem cell linesGene expression dataIrradiated adult miceHox genesPluripotent stateBlood lineagesSomatic cellsEctopic expressionCombinatorial overexpressionExpression dataBlood formationIPS cellsColony activityMultilineage reconstitution
2008
Patient-Specific Induced Pluripotent Stem Cells in Hurler Syndrome
Tolar J, Park I, Xia L, Osborn M, McElmurry R, Orchard P, Daley G, Blazar B. Patient-Specific Induced Pluripotent Stem Cells in Hurler Syndrome. Blood 2008, 112: 386. DOI: 10.1182/blood.v112.11.386.386.Peer-Reviewed Original ResearchGreen fluorescent proteinPluripotent stem cellsIPS cellsStem cellsMesenchymal stromal cellsTumor rejection antigen-1Such induced pluripotent stem cellsWild-type levelsAutologous pluripotent cellsTransient mRNA expressionInduced pluripotent stem cellsMouse embryo fibroblastsStage-specific embryonic antigen-3Adult stem cellsEmbryonic stem cell isolationHematopoietic progenyHematopoietic stem cellsStable transgenesisTransposon insertionMesodermal lineagesNumerous tissue typesSleeping Beauty transposon systemTranscription factorsNon-viral Sleeping Beauty (SB) transposon systemPluripotent cells