2023
Generation of ventralized human thalamic organoids with thalamic reticular nucleus
Kiral F, Cakir B, Tanaka Y, Kim J, Yang W, Wehbe F, Kang Y, Zhong M, Sancer G, Lee S, Xiang Y, Park I. Generation of ventralized human thalamic organoids with thalamic reticular nucleus. Cell Stem Cell 2023, 30: 677-688.e5. PMID: 37019105, PMCID: PMC10329908, DOI: 10.1016/j.stem.2023.03.007.Peer-Reviewed Original ResearchConceptsHuman embryonic stem cellsSingle-cell RNA sequencingReceptor tyrosine protein kinaseTyrosine protein kinaseEmbryonic stem cellsDisease-associated genesLineage developmentRNA sequencingHuman brain developmentOrganoid systemsStem cellsHuman brain organoidsNeuronal functionBrain organoidsOrganoidsBrain organoid systemsDistinct nucleiBrain developmentThalamic developmentPTCHD1NucleusKinaseGenesSequencing
2022
Dyslexia associated gene KIAA0319 regulates cell cycle during human neuroepithelial cell development
Paniagua S, Cakir B, Hu Y, Kiral FR, Tanaka Y, Xiang Y, Patterson B, Gruen JR, Park IH. Dyslexia associated gene KIAA0319 regulates cell cycle during human neuroepithelial cell development. Frontiers In Cell And Developmental Biology 2022, 10: 967147. PMID: 36016658, PMCID: PMC9395643, DOI: 10.3389/fcell.2022.967147.Peer-Reviewed Original ResearchHuman embryonic stem cellsEmbryonic stem cellsGenetic linkage analysisNeuroepithelial cell differentiationHESC differentiationRegulatory elementsChromosome 6p22Human cortical developmentCell cycleMolecular mechanismsAssociation studiesCell developmentCell differentiationLinkage analysisNeuronal migrationStem cellsDYX2 locusCortical developmentNeuroectodermal differentiationDifferentiationGene KIAA0319Radial migrationHESCsGenesLociExpression of the transcription factor PU.1 induces the generation of microglia-like cells in human cortical organoids
Cakir B, Tanaka Y, Kiral FR, Xiang Y, Dagliyan O, Wang J, Lee M, Greaney AM, Yang WS, duBoulay C, Kural MH, Patterson B, Zhong M, Kim J, Bai Y, Min W, Niklason LE, Patra P, Park IH. Expression of the transcription factor PU.1 induces the generation of microglia-like cells in human cortical organoids. Nature Communications 2022, 13: 430. PMID: 35058453, PMCID: PMC8776770, DOI: 10.1038/s41467-022-28043-y.Peer-Reviewed Original ResearchConceptsHuman embryonic stem cellsHuman cortical organoidsTranscription factor PUSingle-cell RNA sequencingMicroglia-like cellsSingle-cell transcriptomicsEmbryonic stem cellsDisease stage IIIRole of microgliaAD-associated genesExpression of genesCortical organoidsNeurodegenerative disordersRNA sequencingMolecular damageIntact complementStem cellsDysfunction of microgliaFunctional microgliaReduced expressionGenesCell clustersExpressionChemokine systemHuman microglia
2020
Genes causing congenital hydrocephalus: Their chromosomal characteristics of telomere proximity and DNA compositions
McKnight I, Hart C, Park IH, Shim JW. Genes causing congenital hydrocephalus: Their chromosomal characteristics of telomere proximity and DNA compositions. Experimental Neurology 2020, 335: 113523. PMID: 33157092, PMCID: PMC7750280, DOI: 10.1016/j.expneurol.2020.113523.Peer-Reviewed Original ResearchConceptsCongenital hydrocephalusCentral nervous systemFamilial Parkinson's diseaseAlzheimer's diseaseCausative genesGenome Data ViewerHuman genetic mutationsDisease-susceptible genesHigh mutation rateGenetic mutationsHuman congenital hydrocephalusHuman clinical studiesPutative genesHuman genesGenomic informationT contentChromosomal characteristicsDNA compositionGenetic basisHigh adenineMutation rateClinical studiesGenesPreclinical modelsThymine content
2012
Cellular reprogramming: a novel tool for investigating autism spectrum disorders
Kim KY, Jung YW, Sullivan GJ, Chung L, Park IH. Cellular reprogramming: a novel tool for investigating autism spectrum disorders. Trends In Molecular Medicine 2012, 18: 463-471. PMID: 22771169, PMCID: PMC3785941, DOI: 10.1016/j.molmed.2012.06.002.Peer-Reviewed Original ResearchConceptsInduced pluripotent stem cellsNovel ASD genesUse of iPSCsHuman disease modelsPluripotent stem cellsSomatic cellsGenomic technologiesAdvanced geneticsASD genesCellular modelStem cellsScreening platformSmall moleculesDisease modelsNovel toolNeurodevelopmental disordersUnprecedented opportunityCellsGenesGeneticsCell therapyAutism spectrum disorderMurine modelFuture perspectivesReciprocal social interactionThe lesser known story of X chromosome reactivation
Hysolli E, Tanaka Y, Kim K, Jung Y, Park IH. The lesser known story of X chromosome reactivation. Cell Cycle 2012, 11: 229-235. PMID: 22234239, PMCID: PMC3293375, DOI: 10.4161/cc.11.2.18998.Peer-Reviewed Original ResearchConceptsX chromosome reactivationX-chromosome inactivationInner cell massActive histone marksGerm cell formationX inactivation signalHistone marksActive chromosomeCellular reprogrammingInactivation signalGene poolPluripotent cellsKey regulatorFemale cellsGene expressionGerm layersCell formationXY cellsCell massImportant mechanismNatural occurrenceCellsReprogrammingChromosomesGenes
2009
Gene Targeting of a Disease-Related Gene in Human Induced Pluripotent Stem and Embryonic Stem Cells
Zou J, Maeder ML, Mali P, Pruett-Miller SM, Thibodeau-Beganny S, Chou BK, Chen G, Ye Z, Park IH, Daley GQ, Porteus MH, Joung JK, Cheng L. Gene Targeting of a Disease-Related Gene in Human Induced Pluripotent Stem and Embryonic Stem Cells. Cell Stem Cell 2009, 5: 97-110. PMID: 19540188, PMCID: PMC2720132, DOI: 10.1016/j.stem.2009.05.023.Peer-Reviewed Original ResearchConceptsHuman ES cellsZinc finger nucleasesSequence-specific double-strand breaksEngineered zinc finger nucleasesGene targetingGFP reporter geneEmbryonic stem cellsDouble-strand breaksHuman Induced Pluripotent StemSpecific genetic modificationsHuman iPS cellsES cellsFinger nucleasesTransgene expressionInduced pluripotent stemHomologous recombinationGFP geneInsertional mutagenesisReporter geneDifferent genesGenetic modificationIPS cellsDonor DNAGenesCell typesHuman iPS Cell Derivation/Reprogramming
Park I, Daley GQ. Human iPS Cell Derivation/Reprogramming. Current Protocols In Stem Cell Biology 2009, 8: 4a.1.1-4a.1.8. PMID: 19170021, DOI: 10.1002/9780470151808.sc04a01s8.Peer-Reviewed Original ResearchConceptsHES cellsHuman fibroblast cellsInduced pluripotent stem cellsFibroblast cellsHuman iPS cellsPluripotent stem cellsPluripotency genesTranscription factorsEmbryoid bodiesGerm layersIPS cellsStem cell culture conditionsCell coloniesCell culture conditionsStem cellsRetroviral vectorsCulture conditionsCellsReprogrammingGenesDifferentiationColoniesDissection microscope