2024
Telencephalic organoids as model systems to study cortical development and diseases
Yang W, Kiral F, Park I. Telencephalic organoids as model systems to study cortical development and diseases. Organoid 2024, 4: e1. DOI: 10.51335/organoid.2024.4.e1.Peer-Reviewed Original Research
2023
Diencephalic organoids – A key to unraveling development, connectivity, and pathology of the human diencephalon
Kiral F, Choe M, Park I. Diencephalic organoids – A key to unraveling development, connectivity, and pathology of the human diencephalon. Frontiers In Cellular Neuroscience 2023, 17: 1308479. PMID: 38130869, PMCID: PMC10733522, DOI: 10.3389/fncel.2023.1308479.Peer-Reviewed Original ResearchHuman diencephalonBrain organoidsNeurodevelopmental disordersDevelopmental brain disordersHuman brain tissueThalamocortical connectionsBrain disordersDiencephalic developmentBrain tissueDiencephalic structuresOrganoid modelsHuman-specific aspectsSensory processingDiencephalonDisordersTelencephalic fatePathologyStem cellsStem cell technologyOrganoidsGeneration of ventralized human thalamic organoids with thalamic reticular nucleus
Kiral F, Cakir B, Tanaka Y, Kim J, Yang W, Wehbe F, Kang Y, Zhong M, Sancer G, Lee S, Xiang Y, Park I. Generation of ventralized human thalamic organoids with thalamic reticular nucleus. Cell Stem Cell 2023, 30: 677-688.e5. PMID: 37019105, PMCID: PMC10329908, DOI: 10.1016/j.stem.2023.03.007.Peer-Reviewed Original ResearchConceptsHuman embryonic stem cellsSingle-cell RNA sequencingReceptor tyrosine protein kinaseTyrosine protein kinaseEmbryonic stem cellsDisease-associated genesLineage developmentRNA sequencingHuman brain developmentOrganoid systemsStem cellsHuman brain organoidsNeuronal functionBrain organoidsOrganoidsBrain organoid systemsDistinct nucleiBrain developmentThalamic developmentPTCHD1NucleusKinaseGenesSequencing
2022
Getting the right cells
Cakir B, Park IH. Getting the right cells. ELife 2022, 11: e80373. PMID: 35770899, PMCID: PMC9246363, DOI: 10.7554/elife.80373.Peer-Reviewed Original ResearchRegion Specific Brain Organoids to Study Neurodevelopmental Disorders
Susaimanickam PJ, Kiral FR, Park IH. Region Specific Brain Organoids to Study Neurodevelopmental Disorders. International Journal Of Stem Cells 2022, 15: 26-40. PMID: 35220290, PMCID: PMC8889336, DOI: 10.15283/ijsc22006.Peer-Reviewed Original Research
2021
How well do brain organoids capture your brain?
Kim J, Sullivan GJ, Park IH. How well do brain organoids capture your brain? IScience 2021, 24: 102063. PMID: 33554067, PMCID: PMC7856464, DOI: 10.1016/j.isci.2021.102063.Peer-Reviewed Original Research
2020
Dysregulation of BRD4 Function Underlies the Functional Abnormalities of MeCP2 Mutant Neurons
Xiang Y, Tanaka Y, Patterson B, Hwang SM, Hysolli E, Cakir B, Kim KY, Wang W, Kang YJ, Clement EM, Zhong M, Lee SH, Cho YS, Patra P, Sullivan GJ, Weissman SM, Park IH. Dysregulation of BRD4 Function Underlies the Functional Abnormalities of MeCP2 Mutant Neurons. Molecular Cell 2020, 79: 84-98.e9. PMID: 32526163, PMCID: PMC7375197, DOI: 10.1016/j.molcel.2020.05.016.Peer-Reviewed Original ResearchConceptsMECP2 mutant neuronsEnhancer-promoter interactionsRett syndromeRTT-like phenotypesChromatin bindingMeCP2 functionMethyl-CpGAbnormal transcriptionRTT etiologyMutant neuronsBET inhibitorsPotential therapeutic opportunitiesMECP2 mutationsProtein 2Human brain organoidsFunctional phenotypeJQ1BRD4Therapeutic opportunitiesBrain organoidsFunction underliesMutationsPhenotypeHuman brain culturesCritical driverGeneration of Regionally Specified Human Brain Organoids Resembling Thalamus Development
Xiang Y, Cakir B, Park IH. Generation of Regionally Specified Human Brain Organoids Resembling Thalamus Development. STAR Protocols 2020, 1: 100001. PMID: 33103124, PMCID: PMC7580078, DOI: 10.1016/j.xpro.2019.100001.Peer-Reviewed Original ResearchSynthetic Analyses of Single-Cell Transcriptomes from Multiple Brain Organoids and Fetal Brain
Tanaka Y, Cakir B, Xiang Y, Sullivan GJ, Park IH. Synthetic Analyses of Single-Cell Transcriptomes from Multiple Brain Organoids and Fetal Brain. Cell Reports 2020, 30: 1682-1689.e3. PMID: 32049002, PMCID: PMC7043376, DOI: 10.1016/j.celrep.2020.01.038.Peer-Reviewed Original Research
2019
Engineering of human brain organoids with a functional vascular-like system
Cakir B, Xiang Y, Tanaka Y, Kural MH, Parent M, Kang YJ, Chapeton K, Patterson B, Yuan Y, He CS, Raredon MSB, Dengelegi J, Kim KY, Sun P, Zhong M, Lee S, Patra P, Hyder F, Niklason LE, Lee SH, Yoon YS, Park IH. Engineering of human brain organoids with a functional vascular-like system. Nature Methods 2019, 16: 1169-1175. PMID: 31591580, PMCID: PMC6918722, DOI: 10.1038/s41592-019-0586-5.Peer-Reviewed Original ResearchConceptsHuman cortical organoidsBlood-brain barrier characteristicsTrans-endothelial electrical resistanceVasculature-like structuresHuman brain organoidsHuman brain developmentCortical organoidsFunctional maturationPrenatal brainBrain diseasesBrain developmentHuman embryonic stem cellsBlood vesselsBrain organoidsTight junctionsDiseaseStem cellsOrganoidsVariant 2Nutrient transportersNutrient deliveryCellsEndotheliumMicrovasculature
2018
Generation and Fusion of Human Cortical and Medial Ganglionic Eminence Brain Organoids
Xiang Y, Yoshiaki T, Patterson B, Cakir B, Kim K, Cho YS, Park I. Generation and Fusion of Human Cortical and Medial Ganglionic Eminence Brain Organoids. Current Protocols In Stem Cell Biology 2018, 47 PMID: 30854156, PMCID: PMC6402040, DOI: 10.1002/cpsc.61.Peer-Reviewed Original ResearchConceptsMedial ganglionic eminenceBrain organoidsBrain regionsBrain developmentSpecific brain regionsDifferent brain regionsHuman CorticalHuman brain developmentCortical organoidsGanglionic eminenceNeurological disordersInterneuron migrationSpecific regionalizationOrganoid culturesOrganoidsBrain organoid culture
2017
Fusion of Regionally Specified hPSC-Derived Organoids Models Human Brain Development and Interneuron Migration
Xiang Y, Tanaka Y, Patterson B, Kang YJ, Govindaiah G, Roselaar N, Cakir B, Kim KY, Lombroso AP, Hwang SM, Zhong M, Stanley EG, Elefanty AG, Naegele JR, Lee SH, Weissman SM, Park IH. Fusion of Regionally Specified hPSC-Derived Organoids Models Human Brain Development and Interneuron Migration. Cell Stem Cell 2017, 21: 383-398.e7. PMID: 28757360, PMCID: PMC5720381, DOI: 10.1016/j.stem.2017.07.007.Peer-Reviewed Original ResearchConceptsHuman brain developmentChromatin accessibility dynamicsTransposase-accessible chromatinHigh-throughput sequencing analysisRegion-specific organoidsHuman pluripotent stem cellsRNA sequencing profilingHuman interneuron migrationPluripotent stem cellsRelated lineagesBrain developmentAccessibility dynamicsBulk assaysInterneuron migrationLineage relationshipsOrganoid techniquesSequencing profilingSequencing analysisFunctional neuronsOrganoid developmentStem cellsCortical organoidsOrganoidsBrain organoidsMGE