2021
Roles of piRNAs in transposon and pseudogene regulation of germline mRNAs and lncRNAs
Wang C, Lin H. Roles of piRNAs in transposon and pseudogene regulation of germline mRNAs and lncRNAs. Genome Biology 2021, 22: 27. PMID: 33419460, PMCID: PMC7792047, DOI: 10.1186/s13059-020-02221-x.Peer-Reviewed Original ResearchConceptsPIWI-interacting RNAsPIWI proteinsRole of piRNAsPIWI-piRNA pathwayRNA-binding proteinSmall noncoding RNAsGermline mRNAsGermline developmentNoncoding RNAsRegulatory relationshipsGerm cellsRNAProteinTransposonMajor classesRNA levelsRecent studiesExpressionGenomeSubfamiliesGermlineLncRNAsMajor constituentsMRNARegulationGenome-wide mapping of Piwi association with specific loci in Drosophila ovaries
Liu N, Neuenkirchen N, Zhong M, Lin H. Genome-wide mapping of Piwi association with specific loci in Drosophila ovaries. G3: Genes, Genomes, Genetics 2021, 11: jkaa059. PMID: 33609367, PMCID: PMC8022938, DOI: 10.1093/g3journal/jkaa059.Peer-Reviewed Original ResearchConceptsPIWI-interacting RNAsProtein-coding genesDrosophila ovaryGermline stem cell maintenanceRole of piRNAsTermination sitesGenome-wide mappingGenomic binding profileTranscriptional termination sitesSpecific genomic sitesStem cell maintenanceRNA pathwaysTransposon repressionTranscriptional startEuchromatic regionsGene regulationEpigenetic regulationGenomic sitesCell maintenancePiwiSpecific lociMethylation signalsDiverse mechanismsTarget siteBinding sites
2018
A critical role for nucleoporin 358 (Nup358) in transposon silencing and piRNA biogenesis in Drosophila
Parikh RY, Lin H, Gangaraju VK. A critical role for nucleoporin 358 (Nup358) in transposon silencing and piRNA biogenesis in Drosophila. Journal Of Biological Chemistry 2018, 293: 9140-9147. PMID: 29735528, PMCID: PMC6005430, DOI: 10.1074/jbc.ac118.003264.Peer-Reviewed Original ResearchMeSH KeywordsActive Transport, Cell NucleusAnimalsArgonaute ProteinsDNA Transposable ElementsDrosophilaDrosophila ProteinsFemaleGene Expression RegulationGene SilencingGenomic InstabilityGerm CellsMaleMolecular ChaperonesNuclear Pore Complex ProteinsProtein Interaction MapsRNA, Small InterferingTranscription, GeneticConceptsPIWI-interacting RNAsPing-pong cycleNuclear pore complexPiRNA biogenesisGermline knockdownPiRNA pathwayAntisense Piwi-interacting RNAsPiRNA precursor transcriptionSmall noncoding RNAsPiwi functionSilence transposonsPIWI proteinsShort hairpin RNACritical roleArgonaute 3Pore complexNoncoding RNAsGenomic instabilityNuclear localizationGene expressionTransposonNup358Germ cellsBiogenesisHairpin RNA
2016
Tudor-SN Interacts with Piwi Antagonistically in Regulating Spermatogenesis but Synergistically in Silencing Transposons in Drosophila
Ku HY, Gangaraju VK, Qi H, Liu N, Lin H. Tudor-SN Interacts with Piwi Antagonistically in Regulating Spermatogenesis but Synergistically in Silencing Transposons in Drosophila. PLOS Genetics 2016, 12: e1005813. PMID: 26808625, PMCID: PMC4726654, DOI: 10.1371/journal.pgen.1005813.Peer-Reviewed Original ResearchConceptsPiRNA biogenesisPrimordial germ cellsPiwi expressionTudor-SNSomatic cellsGerm cellsDiverse molecular functionsPost-transcriptional regulationEmbryonic somatic cellsPiwi mutantsDosage-dependent mannerGermline developmentPIWI proteinsMutant phenotypeMeiotic cytokinesisMolecular functionsSpliceosome assemblyPiwiEpigenetic programmingDiverse functionsBiological functionsAdult ovariesBiogenesisTransposonMale fertility
2015
Poreless eggshells
Lin H, Matzuk MM. Poreless eggshells. Journal Of Clinical Investigation 2015, 125: 4005-4007. PMID: 26485282, PMCID: PMC4639988, DOI: 10.1172/jci84692.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAquaporinsDrosophila ProteinsFemaleGonadal Dysgenesis, 46,XXHumansMaleMutation, MissenseNuclear Pore Complex ProteinsOvaryConceptsNuclear pore complex functionMissense mutationsSpecific amino acid changesAmino acid changesRecessive missense mutationSomatic cellsNucleoporin 107Human mutationsGenetic materialAcid changesExtragonadal functionsOvarian developmentOocyte developmentMutationsGenesSole sourceFemale genetic materialIntrinsic factorsComplex functionsOrthologsAbnormal ovarian developmentFollicular developmentEmbryosFliesOocytesPiwi Is a Key Regulator of Both Somatic and Germline Stem Cells in the Drosophila Testis
Gonzalez J, Qi H, Liu N, Lin H. Piwi Is a Key Regulator of Both Somatic and Germline Stem Cells in the Drosophila Testis. Cell Reports 2015, 12: 150-161. PMID: 26119740, PMCID: PMC4497877, DOI: 10.1016/j.celrep.2015.06.004.Peer-Reviewed Original ResearchConceptsGermline stem cellsDrosophila testisSomatic cyst cellsPIWI-piRNA pathwayStem cell maintenanceCell-autonomous functionStem cellsGerm cell differentiationStem cell typesFasciclin 3GSC daughtersGermline functionSomatic rolePiwi expressionCyst cellsCell maintenanceEpigenetic mechanismsPiwiGenes keyNuclear localizationKey regulatorMolecular mechanismsCell differentiationGonadal developmentCell typesReassessment of Piwi Binding to the Genome and Piwi Impact on RNA Polymerase II Distribution
Lin H, Chen M, Kundaje A, Valouev A, Yin H, Liu N, Neuenkirchen N, Zhong M, Snyder M. Reassessment of Piwi Binding to the Genome and Piwi Impact on RNA Polymerase II Distribution. Developmental Cell 2015, 32: 772-774. PMID: 25805139, PMCID: PMC4472434, DOI: 10.1016/j.devcel.2015.03.004.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsArgonaute ProteinsBase SequenceBinding SitesChromatin ImmunoprecipitationChromobox Protein Homolog 5Chromosomal Proteins, Non-HistoneDNA-Binding ProteinsDrosophila melanogasterDrosophila ProteinsGenomeHigh-Throughput Nucleotide SequencingHistone MethyltransferasesHistone-Lysine N-MethyltransferaseMethyltransferasesRNA InterferenceRNA Polymerase IIRNA, Small InterferingSequence Analysis, DNAConceptsRNA polymerase II distributionGenomic targetsHeterochromatin protein 1aRNA polymerase IICurrent bioinformatics methodsPiwi mutantsDrosophila PiwiPolymerase IIDevelopmental cellsPericentric regionsHistone methyltransferaseBioinformatics methodsBioinformatics pipelineProtein 1APiwiGenomePiRNAsEuchromatinMutantsMethyltransferaseSites
2014
Piwi Is Required in Multiple Cell Types to Control Germline Stem Cell Lineage Development in the Drosophila Ovary
Ma X, Wang S, Do T, Song X, Inaba M, Nishimoto Y, Liu LP, Gao Y, Mao Y, Li H, McDowell W, Park J, Malanowski K, Peak A, Perera A, Li H, Gaudenz K, Haug J, Yamashita Y, Lin H, Ni JQ, Xie T. Piwi Is Required in Multiple Cell Types to Control Germline Stem Cell Lineage Development in the Drosophila Ovary. PLOS ONE 2014, 9: e90267. PMID: 24658126, PMCID: PMC3962343, DOI: 10.1371/journal.pone.0090267.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsArgonaute ProteinsCell DifferentiationCell LineageDNA DamageDrosophilaDrosophila ProteinsFemaleOvarySignal TransductionConceptsGerm cell differentiationDrosophila ovaryGermline stem cellsSomatic cellsCell differentiationGerm cellsPiRNA productionMultiple cell typesGSC maintenanceTransposable elementsGerm lineLineage developmentCell typesPiRNA pathway componentsGerm cell maintenanceCell lineage developmentUndifferentiated germ cellsCell-specific knockdownEscort cellsGSC establishmentGSC lossPiwi knockdownPiRNA pathwayGenome stabilityTE transcriptsPIWI proteins and PIWI-interacting RNAs in the soma
Ross RJ, Weiner MM, Lin H. PIWI proteins and PIWI-interacting RNAs in the soma. Nature 2014, 505: 353-359. PMID: 24429634, PMCID: PMC4265809, DOI: 10.1038/nature12987.Peer-Reviewed Original ResearchConceptsPIWI-interacting RNAsPIWI proteinsPIWI-piRNA pathwayDiscovery of millionsWhole-body regenerationStem cell functionSomatic functionsDiverse organismsLower eukaryotesGenome rearrangementsSomatic cellsEpigenetic programmingBiological rolePathwayRNAProteinRecent studiesEukaryotesTransposonOrganismsBiologyUnanticipated dimensionsFunctionCellsRearrangement
2013
PIWI proteins are essential for early Drosophila embryogenesis
Mani SR, Megosh H, Lin H. PIWI proteins are essential for early Drosophila embryogenesis. Developmental Biology 2013, 385: 340-349. PMID: 24184635, PMCID: PMC3915879, DOI: 10.1016/j.ydbio.2013.10.017.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsArgonaute ProteinsDrosophila melanogasterDrosophila ProteinsEmbryonic DevelopmentConceptsPIWI proteinsMitotic defectsEarly embryogenesisArgonaute/PIWI protein familySmall non-coding RNAsEarly Drosophila embryogenesisAsynchronous nuclear divisionPosttranscriptional gene regulationSevere mitotic defectsPIWI protein familyNon-coding RNAsCell cycle progressionCell cycle arrestGermline developmentDrosophila embryogenesisChromatin structureGene regulationSyncytial embryosMitotic machinerySomatic nucleiProtein familyPiwiNuclear divisionNuclear migrationEssential functionsChromatin Immunoprecipitation Assay of Piwi in Drosophila
Yin H, Lin H. Chromatin Immunoprecipitation Assay of Piwi in Drosophila. Methods In Molecular Biology 2013, 1093: 1-11. PMID: 24178552, DOI: 10.1007/978-1-62703-694-8_1.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsArgonaute ProteinsCell NucleusChromatin ImmunoprecipitationDrosophila melanogasterDrosophila ProteinsFemaleGenomicsConceptsSmall RNA pathwaysRNA pathwaysArgonaute protein familyChromatin-associated factorsChromatin immunoprecipitation methodChromatin immunoprecipitation assaysWhole-genome mappingArgonaute proteinsDrosophila PiwiEpigenetic regulationDevelopmental biologyProtein familyImmunoprecipitation assaysCell biologyImmunoprecipitation methodHigh-resolution mapsPiwiEffector componentsBiologyPathwayGeneral utilityDrosophilaEpigenomeBiogenesisEpigeneticsBeyond transposons: the epigenetic and somatic functions of the Piwi-piRNA mechanism
Peng JC, Lin H. Beyond transposons: the epigenetic and somatic functions of the Piwi-piRNA mechanism. Current Opinion In Cell Biology 2013, 25: 190-194. PMID: 23465540, PMCID: PMC3651849, DOI: 10.1016/j.ceb.2013.01.010.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsArgonaute ProteinsDNA Transposable ElementsDrosophila ProteinsEpigenesis, GeneticGene SilencingHistonesRNA, Small InterferingConceptsPIWI-interacting RNAsPIWI-piRNA pathwayBiogenesis of piRNAsSmall non-coding RNAsPost-transcriptional regulationNon-coding RNAsPIWI proteinsGene regulationSomatic cellsPiwi familyGene expressionGeneral mechanismSomatic functionsTransposonPathwayRNARegulationNovel classRecent studiesBiogenesisNew findingsProteinMechanismExpressionFunctionA Major Epigenetic Programming Mechanism Guided by piRNAs
Huang XA, Yin H, Sweeney S, Raha D, Snyder M, Lin H. A Major Epigenetic Programming Mechanism Guided by piRNAs. Developmental Cell 2013, 24: 502-516. PMID: 23434410, PMCID: PMC3600162, DOI: 10.1016/j.devcel.2013.01.023.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnimals, Genetically ModifiedArgonaute ProteinsChromatinChromatin ImmunoprecipitationChromobox Protein Homolog 5Chromosomal Proteins, Non-HistoneDNA Transposable ElementsDrosophilaDrosophila ProteinsEpigenomicsGenomeGenomicsMethyltransferasesMutationReal-Time Polymerase Chain ReactionReverse Transcriptase Polymerase Chain ReactionRNA Polymerase IIRNA, MessengerRNA, Small InterferingConceptsSpecific genomic sitesEpigenetic factorsGenomic sitesRNA polymerase II associationPiwi-piRNA complexDrosophila genomeEpigenetic stateEpigenetic landscapeComplex associatesPiwiGenomePiRNAsCentral enigmaMajor mechanismEctopic sitesSequenceHP1aDrosophilaPiRNASitesEpigeneticsMechanismProgramming mechanismAssociatesRecruitmentFunction of Piwi, a nuclear Piwi/Argonaute protein, is independent of its slicer activity
Darricarrère N, Liu N, Watanabe T, Lin H. Function of Piwi, a nuclear Piwi/Argonaute protein, is independent of its slicer activity. Proceedings Of The National Academy Of Sciences Of The United States Of America 2013, 110: 1297-1302. PMID: 23297219, PMCID: PMC3557079, DOI: 10.1073/pnas.1213283110.Peer-Reviewed Original ResearchConceptsPIWI proteinsPiRNA biogenesisSlicer activityPIWI-interacting RNA (piRNA) biogenesisEndonuclease activityFunction of PiwiNuclear Piwi proteinSecondary piRNA biogenesisGerm line developmentPiwi functionPiwi membersArgonaute proteinsRNA biogenesisArgonaute 3Transgenic fliesPiwiSomatic cellsCatalytic triadEpigenetic factorsRegulatory functionsBiogenesisOnly memberProteinTransposonRecent evidence
2011
A High-Resolution Whole-Genome Map of Key Chromatin Modifications in the Adult Drosophila melanogaster
Yin H, Sweeney S, Raha D, Snyder M, Lin H. A High-Resolution Whole-Genome Map of Key Chromatin Modifications in the Adult Drosophila melanogaster. PLOS Genetics 2011, 7: e1002380. PMID: 22194694, PMCID: PMC3240582, DOI: 10.1371/journal.pgen.1002380.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsChromatin Assembly and DisassemblyChromatin ImmunoprecipitationChromosomal Proteins, Non-HistoneChromosome MappingDrosophila melanogasterDrosophila ProteinsEpigenesis, GeneticEuchromatinGenome, InsectHeterochromatinHigh-Throughput Nucleotide SequencingHistonesRepetitive Sequences, Nucleic AcidRetroelementsRNA Polymerase IITranscription Initiation SiteConceptsHeterochromatin protein 1aChromatin modificationsStart siteChromatin modification landscapeKey chromatin modificationKey histone marksCell typesDrosophila cell typesRNA polymerase IIAdult Drosophila melanogasterTranscriptional start siteDiverse cell typesTranscription start siteFunctionality of genesHigh-Resolution WholeEuchromatic marksHistone codeHistone marksModification landscapeDrosophila melanogasterPolymerase IIGenome mapChromatin immunoprecipitationRegulatory sequencesSplicing junctionsPAPI, a novel TUDOR-domain protein, complexes with AGO3, ME31B and TRAL in the nuage to silence transposition
Liu L, Qi H, Wang J, Lin H. PAPI, a novel TUDOR-domain protein, complexes with AGO3, ME31B and TRAL in the nuage to silence transposition. Development 2011, 138: 1863-1873. PMID: 21447556, PMCID: PMC3074456, DOI: 10.1242/dev.059287.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnimals, Genetically ModifiedArgonaute ProteinsCarrier ProteinsDEAD-box RNA HelicasesDNA Transposable ElementsDrosophila melanogasterDrosophila ProteinsEmbryo, NonmammalianFemaleGene Expression Regulation, DevelopmentalGene SilencingGerm CellsMaleModels, BiologicalMutagenesis, InsertionalPeptide Initiation FactorsProtein BindingRibonucleoproteinsRNA-Binding ProteinsRNA-Induced Silencing ComplexConceptsPiRNA pathway componentsPIWI-interacting RNAsArgonaute 3PiRNA pathwayPIWI proteinsTransposon activationPathway componentsPIWI protein AubergineTudor domain proteinsP-body componentsN-terminal domainNuage componentsPiRNA mutantsTransposon controlGermline developmentTudor domainMutant ovariesArginine methyltransferaseGermline genomeEpigenetic regulationPerinuclear structuresNuageAdult ovariesArginine residuesFunctional interactionMITOPLD Is a Mitochondrial Protein Essential for Nuage Formation and piRNA Biogenesis in the Mouse Germline
Watanabe T, Chuma S, Yamamoto Y, Kuramochi-Miyagawa S, Totoki Y, Toyoda A, Hoki Y, Fujiyama A, Shibata T, Sado T, Noce T, Nakano T, Nakatsuji N, Lin H, Sasaki H. MITOPLD Is a Mitochondrial Protein Essential for Nuage Formation and piRNA Biogenesis in the Mouse Germline. Developmental Cell 2011, 20: 364-375. PMID: 21397847, PMCID: PMC3062204, DOI: 10.1016/j.devcel.2011.01.005.Peer-Reviewed Original ResearchConceptsPiRNA biogenesisDerepression of retrotransposonsPrimary piRNA biogenesisSmall RNA biogenesisMutant germ cellsMitochondrial protein essentialMicrotubule-dependent localizationPiRNA pathwayDrosophila homologRNA biogenesisConserved roleMitoPLDDiverse speciesProtein essentialPerinuclear structuresMouse germlineOuter membraneBiogenesisGerm cellsMeiotic arrestPhospholipase DMetabolism/Phosphatidic acidMitochondriaMutant mice
2010
Drosophila Piwi functions in Hsp90-mediated suppression of phenotypic variation
Gangaraju VK, Yin H, Weiner MM, Wang J, Huang XA, Lin H. Drosophila Piwi functions in Hsp90-mediated suppression of phenotypic variation. Nature Genetics 2010, 43: 153-158. PMID: 21186352, PMCID: PMC3443399, DOI: 10.1038/ng.743.Peer-Reviewed Original ResearchMeSH KeywordsAllelesAnimalsArgonaute ProteinsDNA Transposable ElementsDrosophila melanogasterDrosophila ProteinsElectrophoresis, Gel, Two-DimensionalEpigenesis, GeneticFemaleGene SilencingGenetic VariationGreen Fluorescent ProteinsHSP90 Heat-Shock ProteinsMaleOvaryPhenotypeRNA-Induced Silencing ComplexThe Yb Body, a Major Site for Piwi-associated RNA Biogenesis and a Gateway for Piwi Expression and Transport to the Nucleus in Somatic Cells*
Qi H, Watanabe T, Ku HY, Liu N, Zhong M, Lin H. The Yb Body, a Major Site for Piwi-associated RNA Biogenesis and a Gateway for Piwi Expression and Transport to the Nucleus in Somatic Cells*. Journal Of Biological Chemistry 2010, 286: 3789-3797. PMID: 21106531, PMCID: PMC3030380, DOI: 10.1074/jbc.m110.193888.Peer-Reviewed Original ResearchConceptsYb bodiesSomatic cellsPiRNA pathwayGerm lineEndo-siRNA pathwaySomatic niche cellsTudor-like domainGonadal somatic cellsPutative RNA helicaseCo-immunoprecipitation experimentsSomatic stem cellsFlamenco locusSomatic piRNAsPiRNA biogenesisEndo-siRNAsRNA biogenesisRNA pathwaysPiwi expressionRNA helicaseNovel proteinPiwiNiche cellsMolecular basisStem cellsBiogenesisA Drosophila Chromatin Factor Interacts With the Piwi-Interacting RNA Mechanism in Niche Cells to Regulate Germline Stem Cell Self-Renewal
Smulders-Srinivasan TK, Szakmary A, Lin H. A Drosophila Chromatin Factor Interacts With the Piwi-Interacting RNA Mechanism in Niche Cells to Regulate Germline Stem Cell Self-Renewal. Genetics 2010, 186: 573-583. PMID: 20647505, PMCID: PMC2954472, DOI: 10.1534/genetics.110.119081.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsArgonaute ProteinsChromatinChromosomal Proteins, Non-HistoneDNA-Binding ProteinsDrosophilaDrosophila ProteinsEpigenesis, GeneticFemaleGerm CellsHedgehog ProteinsMalePolycomb-Group ProteinsPolymerase Chain ReactionRepressor ProteinsRNA-Induced Silencing ComplexRNA, Small InterferingSignal TransductionStem Cell NicheStem CellsConceptsGermline stem cellsNiche cellsEpigenetic programmingStem Cell Self-RenewalEnhancer of PolycombTrithorax group genesPotential genetic interactionsCell Self-RenewalNovel epigenetic mechanismStem cellsDrosophila ovaryGSC divisionChromatin factorsNiche signalingChromatin proteinsGenetic interactionsPiwi expressionStem cell defectEpigenetic regulationPiwiEpigenetic mechanismsGroup genesRNA mechanismExpression of hedgehogSelf-Renewal