2019
Promoters to Study Vascular Smooth Muscle
Chakraborty R, Saddouk FZ, Carrao AC, Krause DS, Greif DM, Martin KA. Promoters to Study Vascular Smooth Muscle. Arteriosclerosis Thrombosis And Vascular Biology 2019, 39: 603-612. PMID: 30727757, PMCID: PMC6527360, DOI: 10.1161/atvbaha.119.312449.Peer-Reviewed Original ResearchMeSH KeywordsActinsAnimalsCell LineCell LineageCell TransdifferentiationGene Expression RegulationGene Knockout TechniquesGene TargetingHumansMiceMicrofilament ProteinsMuscle ProteinsMuscle, Smooth, VascularMyocytes, Smooth MuscleMyofibroblastsMyosin Heavy ChainsNeovascularization, PathologicNeovascularization, PhysiologicPhenotypePromoter Regions, GeneticRecombinant Fusion ProteinsConceptsSmooth muscle cellsCre driver linesDiversity of phenotypesMuscle cell typesVisceral smooth muscle cellsSMC transdifferentiationActa2 promoterRemarkable plasticityExciting new eraSMC functionCell typesCre linesEmbryonic heartExciting discoveriesPhenotypeMuscle cellsPerivascular adipocytesPromoterVascular smooth muscleNonmuscular cellsExpressionMyeloid cellsCardiovascular phenotypesCellsBlood vessel wall
2017
SNP in human ARHGEF3 promoter is associated with DNase hypersensitivity, transcript level and platelet function, and Arhgef3 KO mice have increased mean platelet volume
Zou S, Teixeira AM, Kostadima M, Astle WJ, Radhakrishnan A, Simon LM, Truman L, Fang JS, Hwa J, Zhang PX, van der Harst P, Bray PF, Ouwehand WH, Frontini M, Krause DS. SNP in human ARHGEF3 promoter is associated with DNase hypersensitivity, transcript level and platelet function, and Arhgef3 KO mice have increased mean platelet volume. PLOS ONE 2017, 12: e0178095. PMID: 28542600, PMCID: PMC5441597, DOI: 10.1371/journal.pone.0178095.Peer-Reviewed Original ResearchConceptsExpression quantitative lociMK maturationGene expressionRho guanine exchange factorsHuman megakaryocytesGenome-wide association studiesDNase I hypersensitive regionGuanine exchange factorHuman genetic studiesExchange factorReporter mouse modelDNase hypersensitivityQuantitative lociPlatelet traitsMK developmentTranscript levelsCausal SNPsHypersensitive regionARHGEF3Human phenotypesAssociation studiesGenetic studiesHematopoietic subpopulationsGenetic variantsSNPs
2007
Rbm15 Modulates Notch-Induced Transcriptional Activation and Affects Myeloid Differentiation
Ma X, Renda MJ, Wang L, Cheng EC, Niu C, Morris SW, S. AS, Krause DS. Rbm15 Modulates Notch-Induced Transcriptional Activation and Affects Myeloid Differentiation. Molecular And Cellular Biology 2007, 27: 3056-3064. PMID: 17283045, PMCID: PMC1899951, DOI: 10.1128/mcb.01339-06.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsBasic Helix-Loop-Helix Transcription FactorsCell NucleusCHO CellsCricetinaeCricetulusDNA-Binding ProteinsDrosophila ProteinsGene Expression ProfilingHomeodomain ProteinsImmunoglobulin J Recombination Signal Sequence-Binding ProteinMiceMolecular Sequence DataMyeloid CellsMyelopoiesisNuclear ProteinsPromoter Regions, GeneticProtein BindingProtein Structure, TertiaryProtein TransportReceptors, NotchRNA, MessengerRNA, Small InterferingRNA-Binding ProteinsTranscription Factor HES-1Transcription, GeneticTranscriptional ActivationConceptsN-terminusPromoter activityMyeloid differentiationCell linesCell type-specific mannerMyeloid precursor cell linePrimary murine cellsType-specific mannerDominant negative effectStimulation of NotchHematopoietic cell linesHuman erythroleukemia cellsPrecursor cell lineMurine cell linesHematopoietic stem cellsTranscriptional activationHes1 transcriptionRNA interferenceErythroleukemia cellsFusion proteinHes1 promoter activityMurine cellsFusion partnerHematopoietic cellsRBM15
2000
Regulation of CD34 transcription by Sp1 requires sites upstream and downstream of the transcription start site
Taranenko N, Krause D. Regulation of CD34 transcription by Sp1 requires sites upstream and downstream of the transcription start site. Experimental Hematology 2000, 28: 974-984. PMID: 10989198, DOI: 10.1016/s0301-472x(00)00492-6.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntigens, CD34Antigens, NuclearBinding SitesDNADNA HelicasesDNA MethylationDNA ProbesDNA-Binding ProteinsGene Expression RegulationGuanineKu AutoantigenMiceNuclear ProteinsPromoter Regions, GeneticSp1 Transcription FactorTranscription FactorsTranscription, GeneticTransfectionTumor Cells, CulturedUntranslated RegionsConceptsTranscription start sitePromoter activityCD34 promoterStart siteDrosophila S2 cellsHematopoietic cellsTranscription factor bindsDNA binding sitesElectromobility shift assaysSequence-specific mannerS2 cellsTranscriptional regulationMolecular regulationMethylation interferenceDeletion analysisEarly hematopoiesisNuclear proteinsRegulatory regionsFactor bindsShift assaysGene expressionUntranslated regionHematopoietic stemSp1CD34 transcription
1998
CD34 expression by embryonic hematopoietic and endothelial cells does not require c-Myb.
Krause DS, Mucenski ML, Lawler AM, May WS. CD34 expression by embryonic hematopoietic and endothelial cells does not require c-Myb. Experimental Hematology 1998, 26: 1086-92. PMID: 9766450.Peer-Reviewed Original ResearchConceptsC-MybDevelopmental hematopoiesisES cellsPromoter activityHematopoietic cellsD3 embryonic stem cellsStage-specific mannerES cell differentiationEmbryonic stem cellsPrimitive hematopoietic cellsDefinitive hematopoiesisBone marrow stemCell surface glycoproteinBlood islandsEmbryoid bodiesEndothelial cellsTransient transfectionCell differentiationEmbryosStem cellsProgenitor cellsYolk sacHematopoiesisNonhematopoietic cellsCD34 expression