1999
A nuclear factor Y (NFY) site positively regulates the human CD34 stem cell gene.
Radomska H, Satterthwaite A, Taranenko N, Narravula S, Krause D, Tenen D. A nuclear factor Y (NFY) site positively regulates the human CD34 stem cell gene. Blood 1999, 94: 3772-80. PMID: 10572091, DOI: 10.1182/blood.v94.11.3772.423k19_3772_3780.Peer-Reviewed Original ResearchConceptsHuman CD34 geneUntranslated regionCD34 geneC-MybPromoter activityCell-cycle regulated expressionMurine CD34 geneC-Myb binding sitesElectrophoretic mobility shift assaysNuclear factor Y (NF-Y) sitesTranscription factor NFYCCAAT box motifStem cell genesMobility shift assaysOptimal promoter activityReporter gene activityAntibody supershift experimentsEarly hematopoietic cellsTransient transfection experimentsBox motifCombinatorial actionGene activityPromoter functionOligonucleotide competitionCis elementsA Nuclear Factor Y (NFY) Site Positively Regulates the Human CD34 Stem Cell Gene
Radomska H, Satterthwaite A, Taranenko N, Narravula S, Krause D, Tenen D. A Nuclear Factor Y (NFY) Site Positively Regulates the Human CD34 Stem Cell Gene. Blood 1999, 94: 3772-3780. DOI: 10.1182/blood.v94.11.3772.Peer-Reviewed Original ResearchHuman CD34 geneCD34 geneUntranslated regionC-MybPromoter activityCell-cycle regulated expressionMurine CD34 geneC-Myb binding sitesElectrophoretic mobility shift assaysNuclear factor Y (NF-Y) sitesTranscription factor NFYCCAAT box motifStem cell genesMobility shift assaysOptimal promoter activityReporter gene activityAntibody supershift experimentsEarly hematopoietic cellsTransient transfection experimentsBox motifCombinatorial actionGene activityPromoter functionOligonucleotide competitionShift assays
1997
Regulation of CD34 expression in differentiating M1 cells.
Krause DS, Kapadia SU, Raj NB, May WS. Regulation of CD34 expression in differentiating M1 cells. Experimental Hematology 1997, 25: 1051-61. PMID: 9293902.Peer-Reviewed Original ResearchMeSH Keywords3T3 CellsAnimalsAntigens, CD34Base SequenceBinding SitesCell DifferentiationCells, CulturedDNA-Binding ProteinsDown-RegulationGene Expression RegulationGene Expression Regulation, DevelopmentalGene Expression Regulation, NeoplasticHematopoiesisLeukemia, MyeloidMiceMolecular Sequence DataNuclear ProteinsRNA, MessengerTranscription, GeneticConceptsTranscription initiation siteUntranslated regionPromoter activityHematopoietic stemCell type-specific expressionSecondary structureTATA-less promoterPromoter-luciferase reporter constructsFull promoter activityUpstream genomic DNAProgenitor cellsTranslation start siteMature blood cellsType-specific expressionOptimal promoter activityExtensive secondary structureP1 nuclease digestionCell-specific factorsTranscriptional initiationGene regulationTranscription factorsConsensus sitesStart siteRegulatory elementsTATA element