2011
MEKK3 Regulates IFN-γ Production in T Cells through the Rac1/2-Dependent MAPK Cascades
Wang X, Zhang F, Chen F, Liu D, Zheng Y, Zhang Y, Dong C, Su B. MEKK3 Regulates IFN-γ Production in T Cells through the Rac1/2-Dependent MAPK Cascades. The Journal Of Immunology 2011, 186: 5791-5800. PMID: 21471448, PMCID: PMC3833674, DOI: 10.4049/jimmunol.1002127.Peer-Reviewed Original ResearchMeSH KeywordsAdaptive ImmunityAminoquinolinesAnimalsCD4-Positive T-LymphocytesCell LineFlow CytometryImmunoblottingInterferon-gammaInterleukin-12Interleukin-18Listeria monocytogenesMAP Kinase Kinase Kinase 3MAP Kinase Signaling SystemMiceMice, Inbred C57BLMice, KnockoutMitogen-Activated Protein KinasesPyrimidinesRac GTP-Binding ProteinsRac1 GTP-Binding ProteinReceptors, Antigen, T-CellConceptsSer/Thr proteinMAPK activationMAPK kinase kinase (MAP3K) familyP38 MAPK activationThr proteinMAPK cascadeKinase familyMAPK signalsConditional knockout miceMEKK3 activationMEKK3 deficiencyMEKK3Cell differentiationTCR stimulationTCR signalsTh2 cell differentiationSpecific roleBacterial infectionsT cellsCellsKnockout miceActivationJNKProductionProteinmTOR Complex 2 Targets Akt for Proteasomal Degradation via Phosphorylation at the Hydrophobic Motif*
Wu YT, Ouyang W, Lazorchak AS, Liu D, Shen HM, Su B. mTOR Complex 2 Targets Akt for Proteasomal Degradation via Phosphorylation at the Hydrophobic Motif*. Journal Of Biological Chemistry 2011, 286: 14190-14198. PMID: 21321111, PMCID: PMC3077620, DOI: 10.1074/jbc.m111.219923.Peer-Reviewed Original ResearchConceptsAkt Ser-473 phosphorylationSer-473 phosphorylationAkt activationMotif phosphorylationHydrophobic motifProteasomal degradationHydrophobic motif phosphorylationLys-48-linked polyubiquitinationPhosphorylation-dependent ubiquitinationRapid proteasomal degradationProtein kinase AktRapamycin complex 2Protein life cycleDiverse human diseasesFull Akt activationActivity of AktNegative feedback regulationCellular stimuliKinase AktDependent phosphorylationProtein degradationTarget AktAkt activityHuman diseasesAkt protein
2008
The mammalian target of rapamycin complex 2 controls folding and stability of Akt and protein kinase C
Facchinetti V, Ouyang W, Wei H, Soto N, Lazorchak A, Gould C, Lowry C, Newton AC, Mao Y, Miao RQ, Sessa WC, Qin J, Zhang P, Su B, Jacinto E. The mammalian target of rapamycin complex 2 controls folding and stability of Akt and protein kinase C. The EMBO Journal 2008, 27: 1932-1943. PMID: 18566586, PMCID: PMC2486276, DOI: 10.1038/emboj.2008.120.Peer-Reviewed Original ResearchConceptsTarget of rapamycinProtein kinase CTOR complex 1Functions of TORRapamycin-sensitive TOR complex 1Site phosphorylationKinase CTurn motif siteProtein synthesisStability of AktConventional protein kinase CTORC2 functionTOR functionTOR pathwayPhosphorylation of AktHM siteKinase domainProtein foldingMotif sitesProteasome degradationNovel functionBasic residuesMammalian targetPhosphorylationAkt