2011
mTOR Complex 2 Targets Akt for Proteasomal Degradation via Phosphorylation at the Hydrophobic Motif*
Wu YT, Ouyang W, Lazorchak AS, Liu D, Shen HM, Su B. mTOR Complex 2 Targets Akt for Proteasomal Degradation via Phosphorylation at the Hydrophobic Motif*. Journal Of Biological Chemistry 2011, 286: 14190-14198. PMID: 21321111, PMCID: PMC3077620, DOI: 10.1074/jbc.m111.219923.Peer-Reviewed Original ResearchConceptsAkt Ser-473 phosphorylationSer-473 phosphorylationAkt activationMotif phosphorylationHydrophobic motifProteasomal degradationHydrophobic motif phosphorylationLys-48-linked polyubiquitinationPhosphorylation-dependent ubiquitinationRapid proteasomal degradationProtein kinase AktRapamycin complex 2Protein life cycleDiverse human diseasesFull Akt activationActivity of AktNegative feedback regulationCellular stimuliKinase AktDependent phosphorylationProtein degradationTarget AktAkt activityHuman diseasesAkt protein
2010
Sin1-mTORC2 Suppresses rag and il7r Gene Expression through Akt2 in B Cells
Lazorchak AS, Liu D, Facchinetti V, Di Lorenzo A, Sessa WC, Schatz DG, Su B. Sin1-mTORC2 Suppresses rag and il7r Gene Expression through Akt2 in B Cells. Molecular Cell 2010, 39: 433-443. PMID: 20705244, PMCID: PMC2957800, DOI: 10.1016/j.molcel.2010.07.031.Peer-Reviewed Original ResearchMeSH KeywordsAdaptor Proteins, Signal TransducingAnimalsB-LymphocytesCell Line, TransformedDNA-Binding ProteinsForkhead Box Protein O1Forkhead Transcription FactorsGene Expression RegulationGene Rearrangement, B-LymphocyteHomeodomain ProteinsMiceMice, KnockoutPhosphatidylinositol 3-KinasesProto-Oncogene Proteins c-aktReceptors, Interleukin-7Signal TransductionTOR Serine-Threonine KinasesTranscription FactorsConceptsB cell developmentGene expressionCell developmentRAG gene expressionMTOR complex 2FOXO1 transcriptional activityPI3K signalingMTOR inhibitor rapamycinTranscriptional activityKey regulatorB cellsMolecular mechanismsInhibitor rapamycinK signalingCell survivalFoxO1 phosphorylationMammalian targetRecombinase activityPI3KIL-7 receptorAkt2SignalingRapamycinExpressionCells
2006
SIN1/MIP1 Maintains rictor-mTOR Complex Integrity and Regulates Akt Phosphorylation and Substrate Specificity
Jacinto E, Facchinetti V, Liu D, Soto N, Wei S, Jung SY, Huang Q, Qin J, Su B. SIN1/MIP1 Maintains rictor-mTOR Complex Integrity and Regulates Akt Phosphorylation and Substrate Specificity. Cell 2006, 127: 125-137. PMID: 16962653, DOI: 10.1016/j.cell.2006.08.033.Peer-Reviewed Original ResearchMeSH KeywordsAdaptor Proteins, Signal TransducingAnimalsCarrier ProteinsCells, CulturedFibroblastsForkhead Box Protein O1Forkhead Box Protein O3Forkhead Transcription FactorsGene SilencingHumansMiceMice, Inbred C57BLMice, KnockoutMultiprotein ComplexesPhosphoproteinsPhosphorylationProtein KinasesProteinsProto-Oncogene Proteins c-aktRapamycin-Insensitive Companion of mTOR ProteinRegulatory-Associated Protein of mTORSignal TransductionSubstrate SpecificityThreonineTOR Serine-Threonine KinasesTranscription FactorsConceptsAkt Ser473 phosphorylationAkt targetsSer473 phosphorylationAkt/PKB Ser473 phosphorylationPKB Ser473 phosphorylationRecent biochemical studiesTORC2 functionTORC1 functionHydrophobic motifProtein complexesRaptor-mTORMTOR functionActivation loopPhosphorylation sitesAkt functionSubstrate specificityComplex integrityS6KRegulatory mechanismsCell survivalPhosphorylationMammalian targetPhysiological importanceAkt phosphorylationBiochemical studies