1999
Recombinant Vesicular Stomatitis Virus Expressing Respiratory Syncytial Virus (RSV) Glycoproteins: RSV Fusion Protein Can Mediate Infection and Cell Fusion
Kahn J, Schnell M, Buonocore L, Rose J. Recombinant Vesicular Stomatitis Virus Expressing Respiratory Syncytial Virus (RSV) Glycoproteins: RSV Fusion Protein Can Mediate Infection and Cell Fusion. Virology 1999, 254: 81-91. PMID: 9927576, DOI: 10.1006/viro.1998.9535.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsBinding SitesCell LineCricetinaeCytoplasmGene ExpressionGenetic VectorsGlycoproteinsHN ProteinHumansMembrane FusionMolecular Sequence DataRecombinant Fusion ProteinsRespiratory Syncytial Virus, HumanTumor Cells, CulturedVesicular stomatitis Indiana virusViral Envelope ProteinsViral Fusion ProteinsViral ProteinsVirionConceptsRecombinant vesicular stomatitis virusVesicular stomatitis virusRSV fusion proteinRSV F glycoproteinRSV vaccineF recombinantsRSV glycoproteinsRSV proteinsEnvelope glycoproteinVirus attachmentFusion (F) envelope glycoproteinsF glycoproteinG glycoproteinStomatitis virusLarge syncytiaGlycoproteinPH-independent pathwayCell surfaceCell fusionCytoplasmic tail sequencesVSV G.Endosomal pHFusion activityVaccineFusion proteinRedesign and Genetic Dissection of The Rhabdoviruses
Roberts A, Rose J. Redesign and Genetic Dissection of The Rhabdoviruses. Advances In Virus Research 1999, 53: 301-319. PMID: 10582104, DOI: 10.1016/s0065-3527(08)60353-x.Peer-Reviewed Original ResearchConceptsNegative-strand RNA virusesGenetic dissectionNegative-strand genome RNAWild-type virusRNA virusesNonsegmented negative-strand RNA virusesBacteriophage T7 RNA polymeraseNegative-strand RNA genomeVaccine vector developmentT7 RNA polymeraseRNA polymeraseInfection of cellsT7 promoterGenome RNARNA genomeGenetic analysisPolymerase proteinNNS virusesViral transcriptionNotorious pathogenViral assemblyRhabdovirusDNA copiesRecombinant virusesPlasmid DNA
1997
Construction of a Novel Virus That Targets HIV-1-Infected Cells and Controls HIV-1 Infection
Schnell M, Johnson J, Buonocore L, Rose J. Construction of a Novel Virus That Targets HIV-1-Infected Cells and Controls HIV-1 Infection. Cell 1997, 90: 849-857. PMID: 9298897, DOI: 10.1016/s0092-8674(00)80350-5.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCD4 AntigensCricetinaeGene DeletionGene Expression Regulation, ViralGlycoproteinsGTP-Binding ProteinsHIV InfectionsHIV-1HumansJurkat CellsKidneyMembrane GlycoproteinsMembrane ProteinsMicroscopy, ImmunoelectronMutagenesisReceptors, CXCR4Receptors, HIVRecombinant Fusion ProteinsVesicular stomatitis Indiana virusViral Envelope ProteinsVirus ReplicationConceptsHIV-1-infected cellsHIV-1HIV-1 receptors CD4HIV viral loadHIV-1 infectionInfectious HIV-1Recombinant vesicular stomatitis virusT cell linesHIV infectionViral loadVesicular stomatitis virusTherapeutic valueReceptor CD4Targeted virusInfectionVirusEnvelope proteinCell linesStomatitis virusNormal cellsNovel virusCellsGlycoprotein geneCD4CXCR4
1996
Foreign glycoproteins expressed from recombinant vesicular stomatitis viruses are incorporated efficiently into virus particles.
Schnell M, Buonocore L, Kretzschmar E, Johnson E, Rose J. Foreign glycoproteins expressed from recombinant vesicular stomatitis viruses are incorporated efficiently into virus particles. Proceedings Of The National Academy Of Sciences Of The United States Of America 1996, 93: 11359-11365. PMID: 8876140, PMCID: PMC38062, DOI: 10.1073/pnas.93.21.11359.Peer-Reviewed Original ResearchConceptsVSV G proteinDifferent membrane proteinsVesicular stomatitis virusG proteinsMembrane proteinsMembrane protein purificationEctodomain of CD4Virus particlesStomatitis virusWild-type virionsVirus fusion proteinExtra genesHybrid proteinCytoplasmic tailHelical nucleocapsidMammalian cellsRecombinant vesicular stomatitis virusVSV G.Fusion proteinMeasles virus fusion proteinSoluble proteinMembrane envelopeCell surfaceProtein purificationViral targeting
1994
Stimulation of Heterologous Protein Degradation by the Vpu Protein of HIV-1 Requires the Transmembrane and Cytoplasmic Domains of CD4
Buonocore L, Turi T, Crise B, Rose J. Stimulation of Heterologous Protein Degradation by the Vpu Protein of HIV-1 Requires the Transmembrane and Cytoplasmic Domains of CD4. Virology 1994, 204: 482-486. PMID: 8091684, DOI: 10.1006/viro.1994.1560.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceBase SequenceCD4 AntigensGlycoproteinsHeLa CellsHIV-1Human Immunodeficiency Virus ProteinsHumansMembrane GlycoproteinsMolecular Sequence DataProtein Structure, TertiaryRecombinant Fusion ProteinsRecombinant ProteinsViral Envelope ProteinsViral Regulatory and Accessory ProteinsConceptsCytoplasmic domainTransmembrane domainHybrid proteinHeterologous protein degradationVesicular stomatitis virus glycoproteinRapid degradationAdditional hybridsProtein degradationExtracellular domainProtein VpuRelated sequencesVpu proteinDegradation systemEndoplasmic reticulumVSV GVpu expressionProteinVpuTransmembraneVirus glycoproteinRecent studiesDomainHuman immunodeficiency virus type 1Immunodeficiency virus type 1Degradation
1993
Folding and Assembly of Viral Membrane Proteins
Doms R, Lamb R, Rose J, Helenius A. Folding and Assembly of Viral Membrane Proteins. Virology 1993, 193: 545-562. PMID: 8460475, DOI: 10.1006/viro.1993.1164.Peer-Reviewed Original ResearchConceptsViral membrane proteinsQuality control mechanismsMolecular chaperonesGRP78-BiPMembrane proteinsER molecular chaperonesEffects of mutationsMisfolded proteinsProtein transportConformational maturationMisfolded moleculesProtein foldingEnergy-driven processChaperonesProtein structureMolecular mechanismsER environmentGRP78 synthesisExogenous proteinsNascent moleculesProteinDirect roleStructural variabilityControl mechanismsExperimental strategies
1991
Membrane fusion activity, oligomerization, and assembly of the rabies virus glycoprotein
Whitt M, Buonocor L, Prehaud C, Rose J. Membrane fusion activity, oligomerization, and assembly of the rabies virus glycoprotein. Virology 1991, 185: 681-688. PMID: 1660200, DOI: 10.1016/0042-6822(91)90539-n.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntigens, ViralBase SequenceCell LineCentrifugation, Density GradientCricetinaeFlow CytometryGenetic Complementation TestGlycoproteinsHumansHydrogen-Ion ConcentrationKineticsMacromolecular SubstancesMembrane FusionMembrane GlycoproteinsMiceMolecular Sequence DataPlasmidsRabies virusRecombinant Fusion ProteinsVesicular stomatitis Indiana virusViral Envelope ProteinsViral Fusion ProteinsConceptsVSV G proteinG protein trimersMembrane fusion activityVirus G proteinG proteinsRabies G proteinFusion activityHybrid proteinProtein trimerVesicular stomatitis virus G proteinVirus glycoproteinRabies virus glycoproteinCytoplasmic domainMembrane fusionExtracellular domainHeLa cellsRabies virus G proteinCell surfaceProteinVSV particlesSucrose gradientsVSV infectivityGlycoproteinSpike glycoproteinChemical crosslinking
1985
Incorporation of a charged amino acid into the membrane-spanning domain blocks cell surface transport but not membrane anchoring of a viral glycoprotein.
Adams G, Rose J. Incorporation of a charged amino acid into the membrane-spanning domain blocks cell surface transport but not membrane anchoring of a viral glycoprotein. Molecular And Cellular Biology 1985, 5: 1442-1448. PMID: 2993864, PMCID: PMC366875, DOI: 10.1128/mcb.5.6.1442.Peer-Reviewed Original ResearchConceptsMembrane anchoringG proteinsAmino acidsCell surfaceIsoleucine residueMembrane-spanning domainsCell surface transportVesicular stomatitis virus glycoproteinOligonucleotide-directed mutagenesisAmino acid sequenceUncharged amino acidsDetectable protein levelsHydrophobic amino acidsAnimal cellsCDNA clonesIntracellular membranesAcid sequencePunctate patternGolgi regionProteinContinuous stretchVesicular patternProtein levelsViral glycoproteinsVirus glycoprotein
1984
A Cell Line Expressing Vesicular Stomatitis Virus Glycoprotein Fuses at Low pH
Florkiewicz R, Rose J. A Cell Line Expressing Vesicular Stomatitis Virus Glycoprotein Fuses at Low pH. Science 1984, 225: 721-723. PMID: 6087454, DOI: 10.1126/science.6087454.Peer-Reviewed Original Research
1983
Nucleotide sequence of a cDNA clone encoding the entire glycoprotein from the New Jersey serotype of vesicular stomatitis virus
Gallione C, Rose J. Nucleotide sequence of a cDNA clone encoding the entire glycoprotein from the New Jersey serotype of vesicular stomatitis virus. Journal Of Virology 1983, 46: 162-169. PMID: 6298453, PMCID: PMC255104, DOI: 10.1128/jvi.46.1.162-169.1983.Peer-Reviewed Original ResearchConceptsNew Jersey serotypeVesicular stomatitis virusCDNA clonesIndiana serotypeNucleotide sequenceTranslation termination codonVSV serotypesStomatitis virusTransmembrane domainSignal sequenceSerine residuesProtein sequencesTermination codonEsterification sitesGlycosylation sitesNoncoding nucleotidesGlycine residueShort homologiesAmino acidsNucleotidesMRNARabies virusClonesTerminusResidues
1982
Expression from cloned cDNA of cell-surface secreted forms of the glycoprotein of vesicular stomatitis virus in eucaryotic cells
Rose J, Bergmann J. Expression from cloned cDNA of cell-surface secreted forms of the glycoprotein of vesicular stomatitis virus in eucaryotic cells. Cell 1982, 30: 753-762. PMID: 6291783, DOI: 10.1016/0092-8674(82)90280-x.Peer-Reviewed Original ResearchConceptsG proteinsVesicular stomatitis virusCOS1 cellsCOOH terminusStomatitis virusMouse L cellsSV40 early promoterSV40 late promoterNormal G proteinsTransmembrane domainCDNA clonesEucaryotic cellsLate promoterEarly promoterPlasmid vectorCell typesAmino acidsProteinLipid bilayersL cellsPromoterTerminusG-DNADNACells
1981
Conditional expression of the vesicular stomatitis virus glycoprotein gene in Escherichia coli.
Rose J, Shafferman A. Conditional expression of the vesicular stomatitis virus glycoprotein gene in Escherichia coli. Proceedings Of The National Academy Of Sciences Of The United States Of America 1981, 78: 6670-6674. PMID: 6273881, PMCID: PMC349111, DOI: 10.1073/pnas.78.11.6670.Peer-Reviewed Original ResearchConceptsGlycoprotein geneEscherichia coliNH2-terminal domainTryptic peptide mappingRegulatory regionsProtein sequencesBacterial plasmidsCOOH terminusGene regionG proteinsConditional expressionAmino acidsProteinHydrophobic segmentsPeptide mappingPlasmidStomatitis virusGenesDeletionColiLethalitySequenceExpressionAntigenic determinantsTerminusNucleotide sequences of the mRNA's encoding the vesicular stomatitis virus G and M proteins determined from cDNA clones containing the complete coding regions
Rose J, Gallione C. Nucleotide sequences of the mRNA's encoding the vesicular stomatitis virus G and M proteins determined from cDNA clones containing the complete coding regions. Journal Of Virology 1981, 39: 519-528. PMID: 6268840, PMCID: PMC171362, DOI: 10.1128/jvi.39.2.519-528.1981.Peer-Reviewed Original ResearchConceptsCDNA clonesAmino acidsSignal peptideNucleotide sequenceVesicular stomatitis virus M proteinFull-length cDNA cloneBasic amino-terminal domainComplete nucleotide sequenceVesicular stomatitis virus mRNAAmino-terminal domainComplete coding sequenceM protein sequencesComplete coding regionSite of glycosylationBasic amino acidsM proteinVesicular stomatitis virus GMembrane associationTransmembrane segmentsG protein mRNALarge hydrophobic domainsTerminal domainCoding sequenceProtein sequencesAsparagine residues
1980
Vesicular stomatitis virus glycoprotein is anchored in the viral membrane by a hydrophobic domain near the COOH terminus
Rose J, Welch W, Sefton B, Esch F, Ling N. Vesicular stomatitis virus glycoprotein is anchored in the viral membrane by a hydrophobic domain near the COOH terminus. Proceedings Of The National Academy Of Sciences Of The United States Of America 1980, 77: 3884-3888. PMID: 6253998, PMCID: PMC349731, DOI: 10.1073/pnas.77.7.3884.Peer-Reviewed Original ResearchConceptsAmino acid sequencePartial amino acid sequenceVesicular stomatitis virus glycoproteinAcid sequenceAmino acidsProtein geneCOOH terminusHydrophobic domainViral membraneVSV G proteinLipid bilayersTerminal portionTerminal amino acid sequenceM protein geneG protein geneG protein sequencesTerminal amino acidsVirus glycoproteinErythrocyte membrane proteinsMembrane proteinsDNA insertsLeader sequenceComplete sequenceProtein sequencesRNA genome