1996
Exclusion of CD45 inhibits activity of p56lck associated with glycolipid-enriched membrane domains.
Rodgers W, Rose J. Exclusion of CD45 inhibits activity of p56lck associated with glycolipid-enriched membrane domains. Journal Of Cell Biology 1996, 135: 1515-1523. PMID: 8978819, PMCID: PMC2133949, DOI: 10.1083/jcb.135.6.1515.Peer-Reviewed Original ResearchConceptsExclusion of CD45Low kinase activityGEM fractionMembrane domainsKinase activitySrc family tyrosine kinasesReservoir of enzymesSpecific membrane domainsActivity of p56lckTyrosine phosphatase CD45Family tyrosine kinasesT cell developmentTX-100-soluble fractionGEM domainsPhosphatase CD45Membrane proteinsKinase specific activityLckTyrosine kinaseMembrane fractionJurkat cellsPeptide mappingP56lckPhosphorylationHyperphosphorylation
1994
Signals Determining Protein Tyrosine Kinase and Glycosyl-Phosphatidylinositol-Anchored Protein Targeting to a Glycolipid-Enriched Membrane Fraction
Rodgers W, Crise B, Rose J. Signals Determining Protein Tyrosine Kinase and Glycosyl-Phosphatidylinositol-Anchored Protein Targeting to a Glycolipid-Enriched Membrane Fraction. Molecular And Cellular Biology 1994, 14: 5384-5391. DOI: 10.1128/mcb.14.8.5384-5391.1994.Peer-Reviewed Original ResearchProtein tyrosine kinasesCertain protein tyrosine kinasesP56 lckTyrosine kinaseMembrane fractionGlycosyl phosphatidylinositolSrc family protein tyrosine kinasesFamily protein tyrosine kinasesAnalysis of mutantsN-terminal myristateCy-3Glycolipid-enriched membranesAssociation of p56lckCys-5Membrane domainsMembrane proteinsAnchored proteinsGPI anchorKinaseCell typesHeLa cellsLckMDCK cellsGPIProteinSignals Determining Protein Tyrosine Kinase and Glycosyl-Phosphatidylinositol-Anchored Protein Targeting to a Glycolipid-Enriched Membrane Fraction
Rodgers W, Crise B, Rose J. Signals Determining Protein Tyrosine Kinase and Glycosyl-Phosphatidylinositol-Anchored Protein Targeting to a Glycolipid-Enriched Membrane Fraction. Molecular And Cellular Biology 1994, 14: 5384-5391. DOI: 10.1128/mcb.14.8.5384-5391.1994.Peer-Reviewed Original ResearchGPI-anchored proteinsProtein tyrosine kinasesGlycolipid-enriched membraneP56 LckTyrosine kinaseMembrane fractionCys-3Glycosyl-phosphatidylinositol (GPI)-anchored membrane proteinsGlycolipid-enriched membrane fractionCys-5Src family protein tyrosine kinasesTriton X-100-insolubleAnalysis of mutantsGlycosyl-phosphatidylinositol-anchored proteinsN-terminal myristateAssociation of p56lckGPI anchorTransmembrane signalingMembrane proteinsMembrane domainsHeLa cellsKinaseLckProteinTarget signal
1990
Short Related Sequences in the Cytoplasmic Domains of CD4 and CD8 Mediate Binding to the Amino-Terminal Domain of the p56 lck Tyrosine Protein Kinase
Shaw A, Chalupny J, Whitney J, Hammond C, Amrein K, Kavathas P, Sefton B, Rose J. Short Related Sequences in the Cytoplasmic Domains of CD4 and CD8 Mediate Binding to the Amino-Terminal Domain of the p56 lck Tyrosine Protein Kinase. Molecular And Cellular Biology 1990, 10: 1853-1862. DOI: 10.1128/mcb.10.5.1853-1862.1990.Peer-Reviewed Original ResearchAmino-terminal domainTyrosine protein kinaseCytoplasmic domainP56 lckProtein kinaseCommon sequence motifsRelated amino acid sequencesAmino acid sequenceDisulfide bond formationAmino acid residuesSequence motifsHybrid proteinCysteine residuesAcid sequenceUnrelated proteinsRelated sequencesAcid residuesCD4 sequencesLckGlycoprotein CD4HeLa cellsMediate bindingProteinKinaseSequence