2012
Nanoparticle-based artificial RNA silencing machinery for antiviral therapy
Wang Z, Liu H, Yang S, Wang T, Liu C, Cao Y. Nanoparticle-based artificial RNA silencing machinery for antiviral therapy. Proceedings Of The National Academy Of Sciences Of The United States Of America 2012, 109: 12387-12392. PMID: 22802676, PMCID: PMC3412013, DOI: 10.1073/pnas.1207766109.Peer-Reviewed Original ResearchConceptsFundamental gene regulatory mechanismsHepatitis C virusGene regulatory mechanismsCultured cellsTarget RNA cleavageSequence-specific mannerCellular interferon responseFunctional genomicsHuman hepatoma cellsRNA interferenceArtificial RNARegulatory mechanismsMouse modelRNA cleavageCultured human hepatoma cellsSpecific mannerHCV RNA levelsRNAXenotransplantation mouse modelHepatoma cellsInterferon responsePotent antiviral activityRNA levelsMachineryProteinase activity
2011
Identification of the IFITM3 gene as an inhibitor of hepatitis C viral translation in a stable STAT1 cell line
Yao L, Dong H, Zhu H, Nelson D, Liu C, Lambiase L, Li X. Identification of the IFITM3 gene as an inhibitor of hepatitis C viral translation in a stable STAT1 cell line. Journal Of Viral Hepatitis 2011, 18: e523-e529. PMID: 21914072, PMCID: PMC3736357, DOI: 10.1111/j.1365-2893.2011.01452.x.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCarrier ProteinsCell LineGene ExpressionHepacivirusHumansMembrane ProteinsMiceProtein BiosynthesisProtein MultimerizationReal-Time Polymerase Chain ReactionReceptors, EstrogenRecombinant Fusion ProteinsRNA, ViralRNA-Binding ProteinsSTAT1 Transcription FactorTamoxifenTranscriptional ActivationViral ProteinsConceptsFusion proteinCell linesRNA replicationInterferon-induced transmembrane protein 3Internal ribosomal entry siteHCV IRESHCV internal ribosomal entry siteTransmembrane protein 3HCV RNA replicationMouse estrogen receptorSignal transducerTranscription 1Gene expressionViral translationIFITM3 geneHuh7.5 cell lineReal-time PCRAnti-HCV effectGenesEntry siteElevated expressionProtein 3Western blottingIRESProtein
2009
Focal Distribution of Hepatitis C Virus RNA in Infected Livers
Stiffler J, Nguyen M, Sohn J, Liu C, Kaplan D, Seeger C. Focal Distribution of Hepatitis C Virus RNA in Infected Livers. PLOS ONE 2009, 4: e6661. PMID: 19688046, PMCID: PMC2722721, DOI: 10.1371/journal.pone.0006661.Peer-Reviewed Original ResearchMeSH KeywordsBlotting, WesternCohort StudiesGene DosageHepacivirusHepatitis CHumansInterferonsLiverRNA, ViralConceptsHepatitis C virusHCV RNA levelsHCV RNAHCV replicationRNA levelsBiopsy samplesHCV RNA copy numbersIntrahepatic HCV-RNA levelsISG levelsHepatitis C virus RNAWhole liver sectionsC virus RNAViral RNA levelsFocal distributionInnate immune responseRNA copy numberHCV infectionC virusInfected patientsInfected hepatocytesInterferon betaImmune responseInfected liverLiver sectionsIFNbeta expression
2005
Novel type I interferon IL-28A suppresses hepatitis C viral RNA replication
Zhu H, Butera M, Nelson D, Liu C. Novel type I interferon IL-28A suppresses hepatitis C viral RNA replication. Virology Journal 2005, 2: 80. PMID: 16146571, PMCID: PMC1232870, DOI: 10.1186/1743-422x-2-80.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntiviral AgentsBase SequenceCell LineGenes, MHC Class IHepacivirusHepatitis CHumansInterferon Regulatory Factor-1Interferon Type IInterferon-alphaInterferon-Stimulated Gene Factor 3Interleukin-10InterleukinsJanus KinasesMolecular Sequence DataRNA, ViralSignal TransductionSTAT Transcription FactorsVirus ReplicationConceptsInterferon-stimulated genesIL-28AAntiviral activityAntiviral efficacyHuman hepatoma cellsSide effectsChronic hepatitis C viral infectionHepatitis C viral infectionViral RNA replicationAntiviral response ratesHCV subgenomic RNA replicationIFNα-based therapyGenotype 1 infectionHCV chronic infectionC viral infectionIL-10 receptorIL-10 treatmentHLA class IType I IFNJAK-STATRNA replicationDose-dependent mannerHepatoma cellsExpression of ISGsUndesirable side effects
2003
Long‐term interleukin 10 therapy in chronic hepatitis C patients has a proviral and anti‐inflammatory effect
Nelson D, Tu Z, Soldevila‐Pico C, Abdelmalek M, Zhu H, Xu Y, Cabrera R, Liu C, Davis G. Long‐term interleukin 10 therapy in chronic hepatitis C patients has a proviral and anti‐inflammatory effect. Hepatology 2003, 38: 859-868. PMID: 14512873, DOI: 10.1002/hep.1840380412.Peer-Reviewed Original ResearchConceptsIL-10HCV RNASerum ALTCytokine productionChronic hepatitis C patientsFunctional T cell analysesSerum HCV RNA levelsHCV-specific CD4Hepatic inflammation scoresIL-10 administrationInterleukin-10 therapyPBMC cytokine productionTh2 dominant profileHCV RNA levelsHepatitis C patientsT cell analysisTh2 cytokine productionAnti-inflammatory effectsAcute flaresC patientsDisease activityMeq/mLViral burdenAdvanced fibrosisLiver biopsyGene expression associated with interferon alfa antiviral activity in an HCV replicon cell line
Zhu H, Zhao H, Collins C, Eckenrode S, Run Q, McIndoe R, Crawford J, Nelson D, She J, Liu C. Gene expression associated with interferon alfa antiviral activity in an HCV replicon cell line. Hepatology 2003, 37: 1180-1188. PMID: 12717400, DOI: 10.1053/jhep.2003.50184.Peer-Reviewed Original ResearchMeSH KeywordsAntiviral AgentsCarcinoma, HepatocellularDNA-Binding ProteinsGene ExpressionHepacivirusHepatitis C, ChronicHepatocytesHumansInterferon-alphaLiver NeoplasmsRNA, ViralSignal TransductionSTAT1 Transcription FactorSTAT3 Transcription FactorTrans-ActivatorsTumor Cells, CulturedViral ProteinsVirus ReplicationConceptsIFN-alpha antiviral activityIFN-alphaAntiviral activityReplicon cellsHCV replicon cell culture systemChronic hepatitis C viral infectionHepatitis C viral infectionHCV subgenomic RNA replicationHCV replicon cell linesC viral infectionOnly therapeutic optionDirect antiviral activityReplicon cell linesAnti-HCV activityHepatoma cellsDifferent gene expression profilesFeasible experimental modelIFN-alpha signalingCDNA microarray analysisGene expression profilesTherapeutic optionsActivation of STAT3Antiviral efficacyViral infectionResponsive genesInterleukin-1 Inhibits Hepatitis C Virus Subgenomic RNA Replication by Activation of Extracellular Regulated Kinase Pathway
Zhu H, Liu C. Interleukin-1 Inhibits Hepatitis C Virus Subgenomic RNA Replication by Activation of Extracellular Regulated Kinase Pathway. Journal Of Virology 2003, 77: 5493-5498. PMID: 12692250, PMCID: PMC153991, DOI: 10.1128/jvi.77.9.5493-5498.2003.Peer-Reviewed Original ResearchConceptsInterferon-stimulated genesHepatitis C virusInterleukin-1Antiviral activityHCV subgenomic RNA replicationDirect antiviral activityIL-1 productionReplicon cell linesNovel antiviral pathwayViral protein expressionViral clearanceC virusChronic infectionInflammatory processRNA replicationExtracellular-regulated kinase (ERK) pathwayAntiviral pathwaysInhibitory effectProtein expressionCell linesKinase pathwayKinase activationActivationPatientsInfection
2002
Combination of interferon alfa‐2b and ribavirin in liver transplant recipients with histological recurrent hepatitis C
Firpi R, Abdelmalek M, Soldevila‐Pico C, Reed A, Hemming A, Howard R, van der Werf W, Lauwers G, Liu C, Crawford J, Davis G, Nelson D. Combination of interferon alfa‐2b and ribavirin in liver transplant recipients with histological recurrent hepatitis C. Liver Transplantation 2002, 8: 1000-1006. PMID: 12424712, DOI: 10.1053/jlts.2002.34968.Peer-Reviewed Original ResearchConceptsInterferon alfa-2bLiver transplantationAlfa-2bHistological recurrenceViral eradicationHCV RNAWeek 24Recurrent hepatitis C virus (HCV) infectionHCV RNA 6 monthsHepatitis C virus infectionEnd pointProgressive cholestatic diseaseRecurrent HCV infectionSerum HCV RNAC virus infectionLiver transplant recipientsPrimary end pointRecurrent hepatitis CSecondary end pointsGenotype 1 infectionYears of therapyEnd of treatmentHigh viral loadLiver biopsy resultsRecurrent HCV