Child Study Center & Program in Neurodevelopment and Regeneration
Human stem cell modeling of developmental disorders
Flora Vaccarino, PI
A Postdoctoral Associate position is available in the Vaccarino laboratory (https://medicine.yale.edu/lab/vaccarino/) at the Child Study Center, Yale School of Medicine. The lab is in the Sterling Hall of Medicine, in the main Yale Medical School campus
The primary aim of the project is to use induced pluripotent stem cells (iPSC) to investigate the pathogenesis of autism spectrum disorders, Tourette syndrome and related conditions. The lab has generated large numbers of human iPSC lines from over 100 individuals from well-characterized cohorts of patients and their first- degree family members. The research focuses on generating and characterizing neuronal organoid derived from these iPSC lines, and performing multidisciplinary studies of phenotype, transcriptome, epigenome, and synaptic physiology, as well as integrative analyses.
The applicant will be supported by the laboratory’s experience in organoid generation and bioinformatic analyses, and the laboratory’s library of viral vectors to perform cell type-specific tracing and analysis of synaptic connections. The candidate will have the opportunity to collaborate with bioinformaticians who are involved in the analysis of DNA structural and sequence variants. The applicant will also have the opportunity to probe the functional relevance of mutations in coding and noncoding regions by manipulating the genomes of induced pluripotent stem cells using CRISPR technologies to activate/repress enhancers and engineer mutations in iPSCs lines. Finally, the candidate will take advantage of the superb imaging core facilities at Yale University and will interact with experts in several other disciplines, including geneticists, physiologists, and system biologists.
- The candidate must have a Ph.D. and/or M.D., should be an accomplished biomedical scientist with knowledge in molecular and cellular biology and preferably developmental neuroscience.
- Excellent command of the English language is required
Qualified candidates should send a CV, a statement of research interests and the names of two references to:
Vaccarino Lab: https://medicine.yale.edu/lab/vaccarino/
Program in Neurodevelopment and Regeneration: https://medicine.yale.edu/neurodevelopment/