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Applied Biosystems (ABI)

The ABI PRISM 7900HT Sequence Detection System is a high-throughput real-time PCR system that detects and quantitates nucleic acid sequences. Key applications include gene expression quantitation and the detection of single nucleotide polymorphisms (SNPs) using the fluorogenic 5' nuclease assay.
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Fluorescence Detection

To induce fluorescence, the 7900HT system distributes light from an argon-ion laser excitation source to all sample wells. It then directs the resulting fluorescent emission through a spectrograph to a charge-coupled device (CCD) camera. Emission wavelenghts from 500-600 nm are monitored allowing the simultaneous detection of multiple fluorophores. The 7900HT instrumentation is seen below.