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DNA/RNA QC Analysis Service

Specifications

For DNA

We are piloting a program to offer 3 DNA kits. Please see below for specifications. If you anticipate usage, please email the lab at dnasequencing@yale.edu to determine kit availability.

D1000 kit:
  • Sizing Range: 35-1,000 base pairs
  • Quantitative Range: 10-1,000 pg/µL
D5000 kit:
  • Sizing Range: 35-5,000 base pairs
  • Quantitative Range: 10-1,000 pg/µL
Genomic kit:
  • Sizing Range: 200-15,000 base pairs
  • Quantitative Range: 10-100 ng/ul
  • Provides DIN

For RNA (Please submit on ice)

  • Sizing Range: 100-6,000 nt
  • Quantitative Range: 1,000-10,000 pg/ µL
  • Provides RIN

What we need from you

  • 2 µL of sample in a labeled tube (any size/type) – be sure sample buffer contains not more than 7 mM KCl, 20 mM Phosphate Buffer, 20 mM Guanidine-HCl, 80 mM NaCl, or 20 mM Acetate as high salt concentrations will cause electrophoresis failure.

**We will not be responsible for electrophoresis failures due to improperly quantitated samples or samples in buffer with high salt concentrations.

Once we have received your samples

We will run the samples here at our facility at 300 George Street, Room 2127.

Once the analysis is complete, we will email you a report that includes the gel image, electropherogram & Peak Table

Turnaround time for this service is approximately 24 hours.

Agilent Technologies TapeStation 2200

Agilent Technologies TapeStation 2200

Developed by the company behind the industry-leading 2100 Bioanalyzer, the Agilent 2200 TapeStation system offers scalable throughput and rapid results, making it an ideal solution for quality control of biological samples in next-generation sequencing, microarray and qPCR workflows. The TapeStation is a revolutionary tape-based platform for simpler, faster and more reliable electrophoresis. It uses credit card-sized, disposable Agilent ScreenTape that is available for DNA and RNA applications, and low sample volumes, only 2µl of precious sample per run.