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Analyses of "micro" samples

Determination of molar masses for <10micrograms of protein

The solid lines indicate the trace from the RI detector while "dots" are weight average MW for each slice, i.e., measured every 0.5 s. A total of ~7 micrograms of each protein was subjected for analysis

(column: TSK GEL G3000SWXL [TosoHaas], buffer: 20 mM phosphate, 150 mM NaCl, pH=7.5).