Biophysical Analyses
Dr. Ewa Folta-Stogniew sets up a Wyatt HELEOS II multiangle laser light scattering system used to determine the size of proteins, protein complexes, nucleic acids, exosomes, liposomes, and other macromolecules and particles.
Biophysical analyses extend proteomic studies into the functional domain by quantitatively characterizing the thermodynamics and kinetics of protein–protein and protein–ligand interactions and thus, for example, offer the opportunity to leverage protein post-translational modification (PTM) analyses by determining their impact on a protein’s “activities”, including binding affinity, interaction kinetics, and complex formation.
Although the Biophysics Resource within the Keck Foundation Biotechnology Resource closed on December 31, 2025, much of its equipment has been distributed to other Yale laboratories and is available for direct use by Yale/NIDA Neuroproteomics Center investigators (see below). In addition, the Resource’s former director, Dr. Ewa Folta-Stogniew, is available at no charge to perform analyses using instrumentation at Yale University and to assist with interpreting biophysical data generated at Yale or elsewhere.
If you would like to carry out your own analyses, the following list identifies biophysical technologies available at Yale University and the contact person for each instrument:
- For Nanoparticle Tracking Analysis (NTA) and Dynamic Light Scattering (DLS) ,please contact Huanxing Sun, in the CyTOF Facility, TAC-S416, 300 Cedar Street, New Haven, CT
- For Surface Plasmon Resonance (SPR) on a Cytiva/BiaCore T200 instrument ,please contact Ewa Folta-Stogniew, and for the Biacore 8K+ SPR system, please contact Yulia Surovtseva in Yale Center for Molecular Discovery on West Campus, 600 West Campus Drive, West Haven, CT
- For Isothermal Titration Calorimetry (ITC) on a Microcal VP-ITC, please contact Ewa Folta-Stogniew.
- For Circular Dichroism (CD) , Dynamic Light Scattering (DLS) plate reader, and Fluorescence (FL) on a TCSPC TD-Fluor Horiba Fluorolog 3 Time Domain Fluorimeter, please contact Eric Paulson in the Chemical and Biophysical Instrumentation Center (CBIC), 275 Prospect Street, New Haven, CT.
- For the use of SPECTRAmax GEMINI Fluorescence Plate reader, please contact Ewa Folta-Stogniew.
If you would like Dr. Folta-Stogniew to carry out your analyses or to discuss applications of any of the above listed technologies, please contact ewa.folta-stogniew@yale.edu