Surface Plasmon Resonance - BiaCore Biosensor

SPR is a universal tool for studying and characterizing macromolecular interaction in a label-free state. The system detects the binding reaction in real time by monitoring changes in mass concentration at the chip surface; the association and dissociation rate constants are determined directly from the reaction traces. Samples ranging from small molecules to crude extracts, lipid vesicles, viruses, bacteria and eukaryotic cells can be studied in real-time, without the use of labels and with little or no prior sample preparation.

Excellent WebPages about SPR application that address various aspects of SPR application were created by Arnoud Marquart, so there is no excuse not to educate yourself. PLEASE read the SPR basics before scheduling a training session.

Each user should print and READ Biacore T100 hardware (at minimum Chapters 2 and 3) and software (at minimum Chapters: 2, 3, 4, and 5) manuals.

Download and read the "How To" documents so you will have an idea about the general procedures

SPR performs the measurement in solution, but one of the interacting partners is immobilized on the chip surface; no modifications are needed but the measurement requires several consumable that have to be ordered form BiaCore.