2022
A discovery-based proteomics approach identifies protein disulphide isomerase (PDIA1) as a biomarker of β cell stress in type 1 diabetes
Syed F, Singhal D, Raedschelders K, Krishnan P, Bone R, McLaughlin M, Van Eyk J, Mirmira R, Yang M, Mamula M, Wu H, Liu X, Evans-Molina C. A discovery-based proteomics approach identifies protein disulphide isomerase (PDIA1) as a biomarker of β cell stress in type 1 diabetes. EBioMedicine 2022, 87: 104379. PMID: 36463755, PMCID: PMC9719098, DOI: 10.1016/j.ebiom.2022.104379.Peer-Reviewed Original ResearchConceptsT-cell adoptive transferCell adoptive transferNOD miceNOD-SCID miceType 1 diabetesΒ-cell stressAdoptive transferPre-diabetic NOD miceFemale NOD miceNon-diabetic controlsRecent-onset T1DSerum of childrenDistinct mouse modelsΒ-cell functionHuman organ donorsWeeks of agePotential human biomarkersDisease-related changesΒ-cell deathCell protein expressionNOD isletsAutoantibody positivityDiabetes onsetT1D developmentImmune activation
2019
Islet Autoantibody Standardization Program 2018 Workshop: Interlaboratory Comparison of Glutamic Acid Decarboxylase Autoantibody Assay Performance
Lampasona V, Pittman D, Williams A, Achenbach P, Schlosser M, Akolkar B, Winter W, Laboratories P, Watson K, Weets I, Tao Y, Chen V, Yang Y, Uibo R, Reimand K, Knip M, Härkönen T, Chatenoud L, Achenbach P, Neidhoefer S, Schlosser M, Lampasona V, Kawasaki E, Batstra M, Cieremans T, Almås B, Opsion K, Wyka K, Castaño L, Ramelius A, Johansson I, Williams A, Furmaniak J, McDonald T, McLaughlin K, Christie M, Metz A, Mathew A, Hampe C, Lu C, Wasserfall C, Mann C, Pittman D, Ananta J, Yu L, Mamula M, Robinson P, Gaur V, Hagopian W. Islet Autoantibody Standardization Program 2018 Workshop: Interlaboratory Comparison of Glutamic Acid Decarboxylase Autoantibody Assay Performance. Clinical Chemistry 2019, 65: 1141-1152. PMID: 31409598, PMCID: PMC8936135, DOI: 10.1373/clinchem.2019.304196.Peer-Reviewed Original Research
1996
B7 costimulation and autoantigen specificity enable B cells to activate autoreactive T cells.
Roth R, Nakamura T, Mamula M. B7 costimulation and autoantigen specificity enable B cells to activate autoreactive T cells. The Journal Of Immunology 1996, 157: 2924-31. PMID: 8816398, DOI: 10.4049/jimmunol.157.7.2924.Peer-Reviewed Original ResearchMeSH KeywordsAdoptive TransferAnimalsAntibodies, MonoclonalAntigen PresentationAntigens, CDAutoantigensAutoimmunityB7-1 AntigenB7-2 AntigenBase SequenceB-LymphocytesCell MovementCytochrome c GroupCytokinesFemaleGene Expression RegulationImmunizationLymph NodesLymphocyte ActivationMembrane GlycoproteinsMiceMolecular Sequence DataPeptide FragmentsPolymerase Chain ReactionRatsRNA, MessengerSelf ToleranceSignal TransductionT-Lymphocyte SubsetsConceptsAutoreactive T cellsT cell responsesT cellsB cellsIL-4IL-5Self-AgImmunized B-cell-deficient miceCell responsesAutoimmune T cell responsesB cell-deficient miceB7-1 costimulatory moleculeT cell autoimmunityCell-deficient miceTreatment of miceT cell toleranceNaive T cellsCytokine mRNA responsesCell autoimmunityB7 costimulationCostimulatory moleculesAutoantigen specificityB7-2Cytokine mRNAIFN-gamma
1991
Importance of the immune response to the Ro/La particle in the development of congenital heart block and neonatal lupus erythematosus.
Silverman E, Mamula M, Hardin J, Laxer R. Importance of the immune response to the Ro/La particle in the development of congenital heart block and neonatal lupus erythematosus. The Journal Of Rheumatology 1991, 18: 120-4. PMID: 2023181.Peer-Reviewed Original ResearchConceptsNeonatal lupus erythematosusCongenital heart blockAnti-La antibodiesHeart blockImmune responseLupus erythematosusCutaneous neonatal lupus erythematosusAnti-U1RNP antibodiesAnti-Ro antibodiesRo antibodiesAnti-RoLa antibodiesAntibodiesErythematosusAssay techniquesPrevious studiesResponsePatientsInfantsPathogenesisWomen
1987
Implications of anti-Ro/Sjögren's syndrome A antigen autoantibody in normal sera for autoimmunity.
Gaither K, Fox O, Yamagata H, Mamula M, Reichlin M, Harley J. Implications of anti-Ro/Sjögren's syndrome A antigen autoantibody in normal sera for autoimmunity. Journal Of Clinical Investigation 1987, 79: 841-846. PMID: 3546381, PMCID: PMC424215, DOI: 10.1172/jci112892.Peer-Reviewed Original ResearchConceptsRo/SSAAnti-Ro/SSALa/SSBNormal serumAntigen autoantibodiesSjögren's syndromeAnti-Ro/Sjögren's syndromeRo/Sjögren's syndromeHealthy normal donorsSjögren's syndrome autoantibodiesPreexisting immune responseRo/SSA precipitinsTrue autoantibodiesImmune responseNormal donorsAutoantibodiesSyndromeFragments of IgG.Western immunoblotSSA levelsSerumPatientsSensitive assayLow levelsAutoimmunity