2018
Macrophage Migration Inhibitory Factor Regulates U1 Small Nuclear RNP Immune Complex–Mediated Activation of the NLRP3 Inflammasome
Shin MS, Kang Y, Wahl ER, Park HJ, Lazova R, Leng L, Mamula M, Krishnaswamy S, Bucala R, Kang I. Macrophage Migration Inhibitory Factor Regulates U1 Small Nuclear RNP Immune Complex–Mediated Activation of the NLRP3 Inflammasome. Arthritis & Rheumatology 2018, 71: 109-120. PMID: 30009530, PMCID: PMC6310104, DOI: 10.1002/art.40672.Peer-Reviewed Original ResearchMeSH KeywordsAntigen-Antibody ComplexAutoantibodiesBlotting, WesternCARD Signaling Adaptor ProteinsEnzyme-Linked Immunosorbent AssayFlow CytometryHumansInflammasomesInterleukin-1betaIntramolecular OxidoreductasesMacrophage Migration-Inhibitory FactorsMass SpectrometryMonocytesNLR Family, Pyrin Domain-Containing 3 ProteinReceptors, ImmunologicRibonucleoprotein, U1 Small NuclearConceptsMacrophage migration inhibitory factorSystemic lupus erythematosusMigration inhibitory factorNLRP3 inflammasome activationImmune complexesIL-1βNLRP3 inflammasomeHuman monocytesInflammasome activationExpression of MIFProduction of MIFInhibitory factorCaspase-1IL-1β levelsIL-1β productionEnzyme-linked immunosorbentQuantitative polymerase chain reactionHigh expression alleleLupus patientsMIF productionMIF receptorInflammatory characteristicsLupus erythematosusInterleukin-1βPolymerase chain reaction
2017
Macrophage Migration Inhibitory Factor Serves As an Upstream Regulator of NLRP3 Expression and Subsequent IL-1beta Production in Human Monocytes in Response to Lupus U1-snRNP Immune Complex
Shin M, Kang Y, Leng L, Bucala R, Kang I. Macrophage Migration Inhibitory Factor Serves As an Upstream Regulator of NLRP3 Expression and Subsequent IL-1beta Production in Human Monocytes in Response to Lupus U1-snRNP Immune Complex. The Journal Of Immunology 2017, 198: 210.3-210.3. DOI: 10.4049/jimmunol.198.supp.210.3.Peer-Reviewed Original ResearchMacrophage migration inhibitory factorMigration inhibitory factorIL-1β productionImmune complexesIL-1βHuman monocytesNLRP3 inflammasomeProduction of MIFExogenous MIFInhibitory factorMIF gene polymorphismsSerum MIF levelsLupus-prone miceSystemic lupus erythematosusExpression of NLRP3Subsequent tissue injuryIL-1beta productionNF-kB activationCaspase-1 activationMIF antagonistMIF levelsLupus patientsLupus erythematosusNLRP3 expressionAutoantibody production
2013
Self dsDNA induces IL-1β production from human monocytes by activating NLRP3 inflammasome in the presence of anti-dsDNA antibodies (P4083)
Shin M, Kang Y, Lee N, Kim S, Kang K, Lazova R, Kang I. Self dsDNA induces IL-1β production from human monocytes by activating NLRP3 inflammasome in the presence of anti-dsDNA antibodies (P4083). The Journal Of Immunology 2013, 190: 127.13-127.13. DOI: 10.4049/jimmunol.190.supp.127.13.Peer-Reviewed Original ResearchIL-1β productionAnti-dsDNA antibodiesIL-1βHuman monocytesReactive oxygen speciesCell responsesIL-1β dependent mannerPro-inflammatory cytokines IL-1βPeripheral blood mononuclear cellsSelf-nuclear antigensIL-17 productionSystemic lupus erythematosusInnate immune cellsBlood mononuclear cellsPathogenesis of lupusAntibody-positive seraCytokines IL-1βLupus pathogenesisLupus patientsLupus erythematosusAutoimmune responseCutaneous lesionsMononuclear cellsImmune cellsNLRP3 inflammasomeSelf Double-Stranded (ds)DNA Induces IL-1β Production from Human Monocytes by Activating NLRP3 Inflammasome in the Presence of Anti–dsDNA Antibodies
Shin MS, Kang Y, Lee N, Wahl ER, Kim SH, Kang KS, Lazova R, Kang I. Self Double-Stranded (ds)DNA Induces IL-1β Production from Human Monocytes by Activating NLRP3 Inflammasome in the Presence of Anti–dsDNA Antibodies. The Journal Of Immunology 2013, 190: 1407-1415. PMID: 23315075, PMCID: PMC3563755, DOI: 10.4049/jimmunol.1201195.Peer-Reviewed Original ResearchConceptsIL-1β productionIL-1βHuman monocytesReactive oxygen speciesOligomerization domain-like receptor family pyrinCell responsesProinflammatory cytokines IL-1βTh17 cell responseIL-17 productionAnti-dsDNA antibodiesSystemic lupus erythematosusInnate immune cellsAnti-dsDNA AbsPathogenesis of lupusCytokines IL-1βLupus pathogenesisLupus patientsFamily pyrinLupus erythematosusAutoimmune responseCutaneous lesionsImmune cellsT cellsNuclear AgsPathogenic hallmark
2012
U1-Small Nuclear Ribonucleoprotein Activates the NLRP3 Inflammasome in Human Monocytes
Shin MS, Kang Y, Lee N, Kim SH, Kang KS, Lazova R, Kang I. U1-Small Nuclear Ribonucleoprotein Activates the NLRP3 Inflammasome in Human Monocytes. The Journal Of Immunology 2012, 188: 4769-4775. PMID: 22490866, PMCID: PMC3347773, DOI: 10.4049/jimmunol.1103355.Peer-Reviewed Original ResearchConceptsU1 snRNPNuclear ribonucleoproteinSystemic lupus erythematosusIL-1β productionU1 small nuclear RNAU1 small nuclear ribonucleoproteinSmall nuclear ribonucleoproteinCytosolic protein complexesLupus erythematosusAutoimmune diseasesProtein complexesMicrobial nucleic acidsNuclear RNAPre-mRNAHuman monocytesNOD-like receptor familyReactive oxygen speciesEndogenous DNAReceptor familyMolecular complexesOxygen speciesRibonucleoproteinTLR7/8 pathwayIL-1βNuclear moleculesU1-snRNP activates the NLRP3 inflammasome in human monocytes (171.7)
Shin M, Kang Y, Lee N, Kang K, Lazova R, Kang I. U1-snRNP activates the NLRP3 inflammasome in human monocytes (171.7). The Journal Of Immunology 2012, 188: 171.7-171.7. DOI: 10.4049/jimmunol.188.supp.171.7.Peer-Reviewed Original ResearchSystemic lupus erythematosusIL-1β productionU1 snRNPAutoimmune diseasesIL-1βCaspase-1Human monocytesReactive oxygen speciesU1 small nuclear RNAU1 small nuclear ribonucleoproteinCytosolic protein complexesLupus erythematosusNLRP3 inflammasomeMicrobial nucleic acidsNLRP3Protein complexesNuclear RNANuclear ribonucleoproteinPre-mRNAEndogenous DNAMonocytesAntibodiesPotential roleCD14Inflammasome