2021
Long noncoding RNA TINCR is a novel regulator of human bronchial epithelial cell differentiation state
Omote N, Sakamoto K, Li Q, Schupp JC, Adams T, Ahangari F, Chioccioli M, DeIuliis G, Hashimoto N, Hasegawa Y, Kaminski N. Long noncoding RNA TINCR is a novel regulator of human bronchial epithelial cell differentiation state. Physiological Reports 2021, 9: e14727. PMID: 33527707, PMCID: PMC7851438, DOI: 10.14814/phy2.14727.Peer-Reviewed Original ResearchConceptsTerminal differentiation-induced lncRNANormal human bronchial epithelial cellsTINCR overexpressionCell differentiationNotch genesTissue developmentBronchial epithelial cellsExtracellular matrix organizationCell phenotypeRNA sequencing analysisNumerous biological functionsRole of lncRNAsCell differentiation stateEpithelial cellsHuman bronchial epithelial cellsCiliated cell differentiationStaufen1 proteinNovel regulatorBasal cell phenotypeDownstream regulatorsRNA immunoprecipitationBiological functionsCritical regulatorDifferential expressionDifferentiation state
2020
Reconstructed Single-Cell Fate Trajectories Define Lineage Plasticity Windows during Differentiation of Human PSC-Derived Distal Lung Progenitors
Hurley K, Ding J, Villacorta-Martin C, Herriges MJ, Jacob A, Vedaie M, Alysandratos KD, Sun YL, Lin C, Werder RB, Huang J, Wilson AA, Mithal A, Mostoslavsky G, Oglesby I, Caballero IS, Guttentag SH, Ahangari F, Kaminski N, Rodriguez-Fraticelli A, Camargo F, Bar-Joseph Z, Kotton DN. Reconstructed Single-Cell Fate Trajectories Define Lineage Plasticity Windows during Differentiation of Human PSC-Derived Distal Lung Progenitors. Cell Stem Cell 2020, 26: 593-608.e8. PMID: 32004478, PMCID: PMC7469703, DOI: 10.1016/j.stem.2019.12.009.Peer-Reviewed Original ResearchMeSH KeywordsAlveolar Epithelial CellsCell DifferentiationHumansLungPluripotent Stem CellsPulmonary AlveoliConceptsPluripotent stem cellsHuman pluripotent stem cellsLung progenitorsSingle-cell RNACell fate trajectoriesDistal lung progenitorsEndodermal fateLentiviral barcodingSelf-renewal capacityDevelopmental plasticityWnt responseNKX2-1Stem cellsStable phenotypeAlveolar epithelial type 2 cellsProgenitorsFacultative progenitorsPlasticity windowContinuous state Hidden Markov ModelsEpithelial type 2 cellsDifferentiationFateAEC2sType 2 cellsBarcoding