2023
Rhesus macaque Bcl-6/Bcl-xL B cell immortalization: Discovery of HIV-1 neutralizing antibodies from lymph node
Samsel J, Boswell K, Watkins T, Ambrozak D, Mason R, Yamamoto T, Ko S, Yang Y, Zhou T, Doria-Rose N, Foulds K, Roederer M, Mascola J, Kwong P, Gama L, Koup R. Rhesus macaque Bcl-6/Bcl-xL B cell immortalization: Discovery of HIV-1 neutralizing antibodies from lymph node. Journal Of Immunological Methods 2023, 516: 113445. PMID: 36848985, DOI: 10.1016/j.jim.2023.113445.Peer-Reviewed Original ResearchConceptsB cellsLymph nodesRhesus macaquesHIV-1HIV-1 vaccineExpression of CD40Surface B-cell receptorB-cell immortalizationPre-clinical testingB cell receptorIL-21CD40 ligandAntigen specificityBCL-6Cell receptorIdentification of cellsAntibodiesFunctional assaysCell immortalizationBcl-xLAntigen probeCellsRetroviral vectorsSomatic hypermutationAntibody discovery
2022
Application of B cell immortalization for the isolation of antibodies and B cell clones from vaccine and infection settings
Boswell K, Watkins T, Cale E, Samsel J, Andrews S, Ambrozak D, Driscoll J, Messina M, Narpala S, Hopp C, Cagigi A, Casazza J, Yamamoto T, Zhou T, Schief W, Crompton P, Ledgerwood J, Connors M, Gama L, Kwong P, McDermott A, Mascola J, Koup R. Application of B cell immortalization for the isolation of antibodies and B cell clones from vaccine and infection settings. Frontiers In Immunology 2022, 13: 1087018. PMID: 36582240, PMCID: PMC9794141, DOI: 10.3389/fimmu.2022.1087018.Peer-Reviewed Original ResearchConceptsB cell subsetsB cell clonesB-cell immortalizationMemory B cellsB cellsCell subsetsCell clonesHIV-1 infected individualsMemory B cell subsetsDifferent B cell populationsIsolation of antibodiesRecent malaria infectionFuture vaccine designCell immortalizationB cell populationsB cell survivalMalaria infectionPrimary B cellsVaccine settingInfection settingsImmunoglobulin secretionHealthy individualsTonsil tissueAntibody secretionBCL-6