2021
Heterogeneity of meningeal B cells reveals a lymphopoietic niche at the CNS borders
Brioschi S, Wang WL, Peng V, Wang M, Shchukina I, Greenberg ZJ, Bando JK, Jaeger N, Czepielewski RS, Swain A, Mogilenko DA, Beatty WL, Bayguinov P, Fitzpatrick JAJ, Schuettpelz LG, Fronick CC, Smirnov I, Kipnis J, Shapiro VS, Wu GF, Gilfillan S, Cella M, Artyomov MN, Kleinstein SH, Colonna M. Heterogeneity of meningeal B cells reveals a lymphopoietic niche at the CNS borders. Science 2021, 373 PMID: 34083450, PMCID: PMC8448524, DOI: 10.1126/science.abf9277.Peer-Reviewed Original ResearchConceptsCentral nervous systemMeningeal B cellsB cellsAdaptive immune cellsAntigen-experienced B cellsBone marrow chimerasCNS bordersCNS antigensBlood borneImmune privilegeImmune cellsBone marrow nicheParabiosis experimentsSystemic circulationNervous systemB cell developmentLymphopoietic nicheMeningesMarrow nicheVascular connectionsCell developmentCellsConfocal imagingCalvariaImmunosurveillance
2017
Polycomb Repressive Complex 2-Mediated Chromatin Repression Guides Effector CD8+ T Cell Terminal Differentiation and Loss of Multipotency
Gray SM, Amezquita RA, Guan T, Kleinstein SH, Kaech SM. Polycomb Repressive Complex 2-Mediated Chromatin Repression Guides Effector CD8+ T Cell Terminal Differentiation and Loss of Multipotency. Immunity 2017, 46: 596-608. PMID: 28410989, PMCID: PMC5457165, DOI: 10.1016/j.immuni.2017.03.012.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCD8-Positive T-LymphocytesCell DifferentiationChromatinEnhancer of Zeste Homolog 2 ProteinFlow CytometryForkhead Box Protein O1Gene ExpressionHistonesImmunoblottingImmunologic MemoryLysineMethylationMice, Inbred C57BLMice, KnockoutMice, TransgenicModels, ImmunologicalMultipotent Stem CellsPolycomb Repressive Complex 2Reverse Transcriptase Polymerase Chain ReactionConceptsH3K27me3 depositionPolycomb repressive complex 2T cell terminal differentiationRepressive complex 2MP cellsLoss of multipotencyPro-survival genesCell terminal differentiationFate restrictionPermissive chromatinEpigenetic silencingMemory cell potentialDevelopmental plasticityCell developmentTerminal differentiationCell differentiationGenesPrecursor cellsFOXO1 expressionChromatinMemory precursor cellsMultipotencyCell maturationClonal expansionCells
2011
Differential Expression of Ly6C and T-bet Distinguish Effector and Memory Th1 CD4+ Cell Properties during Viral Infection
Marshall HD, Chandele A, Jung YW, Meng H, Poholek AC, Parish IA, Rutishauser R, Cui W, Kleinstein SH, Craft J, Kaech SM. Differential Expression of Ly6C and T-bet Distinguish Effector and Memory Th1 CD4+ Cell Properties during Viral Infection. Immunity 2011, 35: 633-646. PMID: 22018471, PMCID: PMC3444169, DOI: 10.1016/j.immuni.2011.08.016.Peer-Reviewed Original ResearchConceptsAcute viral infectionViral infectionEffector cellsTfh cell markersVirus-specific effectorT helper 1Th1 effector cellsT-bet expressionIL-7R expressionMemory precursor cellsTh1 CD4Helper 1Memory TTh1 cellsProliferative responseSecondary infectionEffector typeReliable markerCell markersInfectionPrecursor cellsGene expression profilesLy6CCell featuresCell developmentInter-follicular germinal center B cell and T follicular helper cell development precedes follicular Tfh maintenance (63.23)
Kerfoot S, Yaari G, Patel J, Johnson K, Gonzalez D, Kleinstein S, Haberman A. Inter-follicular germinal center B cell and T follicular helper cell development precedes follicular Tfh maintenance (63.23). The Journal Of Immunology 2011, 186: 63.23-63.23. DOI: 10.4049/jimmunol.186.supp.63.23.Peer-Reviewed Original ResearchGerminal center B cellsB cellsPD-1T cellsT-cell immigrationFollicular helper cell developmentTfh cell differentiationCognate B cellsDays post immunizationSuch B cellsB cell entryTfh cellsTFH phenotypeInterfollicular zonesPost immunizationGerminal centersBCL-6GL7 expressionCell immigrationLong-term maintenanceCell entryCell developmentFolliclesCell typesCells