2001
Immunological Detection of a Novel Advanced Glycation End-Product
Takeuchi M, Yanase Y, Matsuura N, Yamagishi S, Kameda Y, Bucala R, Makita Z. Immunological Detection of a Novel Advanced Glycation End-Product. Molecular Medicine 2001, 7: 783-791. PMID: 11788793, PMCID: PMC1950006, DOI: 10.1007/bf03401969.Peer-Reviewed Original Research
2000
De Novo Expression of Macrophage Migration Inhibitory Factor in Atherogenesis in Rabbits
Lin S, Yu X, Chen Y, Huang X, Metz C, Bucala R, Lau C, Lan H. De Novo Expression of Macrophage Migration Inhibitory Factor in Atherogenesis in Rabbits. Circulation Research 2000, 87: 1202-1208. PMID: 11110779, DOI: 10.1161/01.res.87.12.1202.Peer-Reviewed Original ResearchConceptsMacrophage migration inhibitory factorUpregulation of MIFSmooth muscle cellsVascular endothelial cellsMIF expressionMigration inhibitory factorFatty streak lesion formationInhibitory factorAbility of MIFIntercellular adhesion molecule-1 expressionAdhesion molecule-1 expressionFoam cell-rich lesionsNew Zealand white rabbitsAccumulation of macrophagesMolecule-1 expressionTranscriptase-polymerase chain reactionHypercholesterolemic rabbit modelEarly fatty streaksZealand white rabbitsDe novo expressionMacrophage-mediated diseasesMIF levelsCholesterol dietMIF mRNANormal dietImmunological Evidence that Non-carboxymethyllysine Advanced Glycation End-products Are Produced from Short Chain Sugars and Dicarbonyl Compounds in vivo
Takeuchi M, Makita Z, Bucala R, Suzuki T, Koike T, Kameda Y. Immunological Evidence that Non-carboxymethyllysine Advanced Glycation End-products Are Produced from Short Chain Sugars and Dicarbonyl Compounds in vivo. Molecular Medicine 2000, 6: 114-125. PMID: 10859028, PMCID: PMC1949938, DOI: 10.1007/bf03401779.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntibodiesCattleChromatography, AffinityCross ReactionsDiabetes Mellitus, Type 2Enzyme-Linked Immunosorbent AssayGlycation End Products, AdvancedGlyceraldehydeGlyoxalHumansImmunoblottingKidney Failure, ChronicLysineMaillard ReactionOxidation-ReductionPyruvaldehydeRabbitsRenal DialysisConceptsAGE antibodyCML-AGEDiabetic patientsAGE-bovine serum albuminShort Chain SugarsAdvanced glycation end productsAnti-AGE antibodyGlycation end productsAutoxidation of sugarsImmunization of rabbitsCML-BSADiabetic serumSerum albuminAdvanced glycationAGE contentSugar autoxidationRabbit serum albuminAGE modificationAntibodiesImmunological evidenceApparent molecular weightPatientsAge 3AgeImmunoblot analysis
1998
Aminoguanidine has an anti-atherogenic effect in the cholesterol-fed rabbit
Panagiotopoulos S, O'Brien R, Bucala R, Cooper M, Jerums G. Aminoguanidine has an anti-atherogenic effect in the cholesterol-fed rabbit. Atherosclerosis 1998, 136: 125-131. PMID: 9544739, DOI: 10.1016/s0021-9150(97)00192-5.Peer-Reviewed Original ResearchConceptsAdvanced glycosylation endproductsAortic archAbdominal aortaAdvanced glycationPlaque formationVasculopathy of diabetesAnti-atherogenic effectsHigh-cholesterol dietCholesterol-fed rabbitsDevelopment of atherosclerosisDegree of atheromaCholesterol dietAortaAtheromaRabbitsThoracicAminoguanidineDosesGlycationTreatmentArchAge levelsNon-enzymatic interactionVasculopathySudan IV
1997
The Pathogenic Role of Macrophage Migration Inhibitory Factor in Immunologically Induced Kidney Disease in the Rat
Lan H, Bacher M, Yang N, Mu W, Nikolic-Paterson D, Metz C, Meinhardt A, Bucala R, Atkins R. The Pathogenic Role of Macrophage Migration Inhibitory Factor in Immunologically Induced Kidney Disease in the Rat. Journal Of Experimental Medicine 1997, 185: 1455-1466. PMID: 9126926, PMCID: PMC2196273, DOI: 10.1084/jem.185.8.1455.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntibodies, MonoclonalAntibody FormationCell Adhesion MoleculesGene ExpressionGlomerulonephritisHypersensitivity, DelayedIntercellular Adhesion Molecule-1Interleukin-1Macrophage Migration-Inhibitory FactorsMaleMiceNitric Oxide SynthaseRabbitsRatsRats, Sprague-DawleyRNA, MessengerSkinTime FactorsVascular Cell Adhesion Molecule-1ConceptsMacrophage migration inhibitory factorControl antibody-treated animalsDelayed-type hypersensitivity responseAnti-MIF treatmentAntibody-treated animalsMigration inhibitory factorAdhesion molecule-1Hypersensitivity responseKidney diseaseLeukocytic infiltrationHistological damageCrescentic anti-glomerular basement membrane (GBM) glomerulonephritisMolecule-1Skin delayed-type hypersensitivity responseInducible nitric oxide synthase (iNOS) expressionAnti-glomerular basement membrane glomerulonephritisInhibitory factorVascular cell adhesion molecule-1Nitric oxide synthase expressionIntercellular adhesion molecule-1Cell adhesion molecule-1Rabbit anti-rat GBM serumProgressive renal injuryRenal function impairmentAnti-GBM glomerulonephritis
1985
Glucocorticoid-lens protein adducts in experimentally induced steroid cataracts
Bucala R, Callati M, Manabe S, Cotlier E, Cerami A. Glucocorticoid-lens protein adducts in experimentally induced steroid cataracts. Experimental Eye Research 1985, 40: 853-863. PMID: 4018168, DOI: 10.1016/0014-4835(85)90130-7.Peer-Reviewed Original Research
1983
Characterization of antisera to the addition product formed by the nonenzymatic reaction of 16α-hydroxyestrone with albumin
Bucala R, Cerami A. Characterization of antisera to the addition product formed by the nonenzymatic reaction of 16α-hydroxyestrone with albumin. Molecular Immunology 1983, 20: 1289-1292. PMID: 6656775, DOI: 10.1016/0161-5890(83)90159-1.Peer-Reviewed Original ResearchConceptsSystemic lupus erythematosusLupus erythematosusAutoimmune diseasesAlpha-hydroxyestroneHigh-titre antiseraLack of specificityCross-reactivity analysisPhenolic A-ringElevated levelsCharacterization of antiseraLow molar substitutionOHEAntiserumImmunochemical analysisHigh specificityAlbuminHigh affinity