2001
Molecular Single-Cell Analysis of Hodgkin- and Reed-Sternberg Cells Harboring Unmutated Immunoglobulin Variable Region Genes
Müschen M, Küppers R, Spieker T, Bräuninger A, Rajewsky K, Hansmann M. Molecular Single-Cell Analysis of Hodgkin- and Reed-Sternberg Cells Harboring Unmutated Immunoglobulin Variable Region Genes. Laboratory Investigation 2001, 81: 289-295. PMID: 11310822, DOI: 10.1038/labinvest.3780237.Peer-Reviewed Original ResearchConceptsImmunoglobulin variable region genesRegion genesVariable region genesGerminal center B cellsSomatic mutationsFounder cellsGerminal center founder cellsB cellsGenesIntrinsic propensityClonal progenyUnmutated immunoglobulin variable region genesClassical Hodgkin's diseaseAntigen-experienced B cellsCell analysisRS cellsMutationsNaive B cellsReed-Sternberg cellsCellsB-lineageLineagesProgenyClonesApoptosisOct-2 and Bob-1 deficiency in Hodgkin and Reed Sternberg cells.
Re D, Müschen M, Ahmadi T, Wickenhauser C, Staratschek-Jox A, Holtick U, Diehl V, Wolf J. Oct-2 and Bob-1 deficiency in Hodgkin and Reed Sternberg cells. Cancer Research 2001, 61: 2080-4. PMID: 11280769.Peer-Reviewed Original ResearchConceptsImmunoglobulin gene expressionH-RS cellsGene expressionOct-2 transcriptsOct-2 proteinTranscription factor Oct-2Primary H-RS cellsCell linesTranscription machineryBob-1Gene deregulationOctamer siteHodgkin's disease-derived cell linesImmunoglobulin genesNovel mechanismGerminal center B cellsCrippling mutationsClassical Hodgkin's diseaseProtein expressionB cellsTranscriptsExpressionProteinReed-Sternberg cellsCellsEvidence that Hodgkin and Reed-Sternberg cells in Hodgkin disease do not represent cell fusions
Küppers R, Bräuninger A, Müschen M, Distler V, Hansmann M, Rajewsky K. Evidence that Hodgkin and Reed-Sternberg cells in Hodgkin disease do not represent cell fusions. Blood 2001, 97: 818-821. PMID: 11157505, DOI: 10.1182/blood.v97.3.818.Peer-Reviewed Original ResearchConceptsHodgkin's diseaseReed-Sternberg cellsHRS cellsT-cell receptor beta rearrangementsCases of HDClassical Hodgkin's diseaseCoexpression of markersCell fusionNumerical chromosomal abnormalitiesUnusual immunophenotypeT cellsRare caseB cellsBeta rearrangementChromosomal abnormalitiesGermline configurationIgH allelesDifferent hematopoietic lineagesDiseaseHodgkinCell generationCellsTCRbeta allelesHematopoietic lineagesImmunoglobulin genes
2000
Rare Occurrence of Classical Hodgkin's Disease as a T Cell Lymphoma
Müschen M, Rajewsky K, Bräuninger A, Baur A, Oudejans J, Roers A, Hansmann M, Küppers R. Rare Occurrence of Classical Hodgkin's Disease as a T Cell Lymphoma. Journal Of Experimental Medicine 2000, 191: 387-394. PMID: 10637283, PMCID: PMC2195757, DOI: 10.1084/jem.191.2.387.Peer-Reviewed Original ResearchConceptsTCR beta locusMature B cellsGene rearrangementsCell-associated proteinsLight chain gene rearrangementsClassical Hodgkin's diseaseDJ gene rearrangementsIg gene rearrangementsSingle-cell polymerase chain reactionIgH locusCases of cHDClonal progenyBeta gene rearrangementsT cell receptorT cell moleculesLociGermline configurationCell phenotypeCell moleculesLineage derivationB cellsRS cellsCell receptorImmunoglobulin heavyCell markers