2001
Interleukin-11 Up-Regulates Survivin Expression in Endothelial Cells through a Signal Transducer and Activator of Transcription-3 Pathway
Mahboubi K, Li F, Plescia J, Kirkiles-Smith N, Mesri M, Du Y, Carroll J, Elias J, Altieri D, Pober J. Interleukin-11 Up-Regulates Survivin Expression in Endothelial Cells through a Signal Transducer and Activator of Transcription-3 Pathway. Laboratory Investigation 2001, 81: 327-334. PMID: 11310826, DOI: 10.1038/labinvest.3780241.Peer-Reviewed Original ResearchMeSH KeywordsDNA-Binding ProteinsEndothelium, VascularGene ExpressionHumansInhibitor of Apoptosis ProteinsInterleukin-11Microtubule-Associated ProteinsNeoplasm ProteinsPhosphorylationProteinsRNA, MessengerSerineSignal TransductionSTAT1 Transcription FactorSTAT3 Transcription FactorSurvivinTrans-ActivatorsTranscription, GeneticTransgenesUmbilical VeinsConceptsSignal transducerProtein kinase B/AktDominant-negative STAT3 mutantActivator of transcriptionSurvivin mRNA expressionTranscription 3 (STAT3) pathwayIL-11Survivin protein expressionSTAT3 mutantStimulation of serumProtein expression levelsSurvivin inductionSurvivin expressionAntiapoptotic proteinsInduces expressionMRNA expressionExpression levelsSurvivin proteinProtein expressionCritical mediatorMaximal inductionExpressionSurvivin mRNAProteinDose-dependent manner
2000
IL-11 Activates Human Endothelial Cells to Resist Immune-Mediated Injury
Mahboubi K, Biedermann B, Carroll J, Pober J. IL-11 Activates Human Endothelial Cells to Resist Immune-Mediated Injury. The Journal Of Immunology 2000, 164: 3837-3846. PMID: 10725745, DOI: 10.4049/jimmunol.164.7.3837.Peer-Reviewed Original ResearchMeSH KeywordsAntigens, CDCells, CulturedComplement System ProteinsCytokine Receptor gp130Cytotoxicity, ImmunologicDNA-Binding ProteinsDose-Response Relationship, ImmunologicEndothelium, VascularEnzyme ActivationHumansImmunity, InnateInflammation MediatorsInterleukin-11Interleukin-11 Receptor alpha SubunitMembrane GlycoproteinsMitogen-Activated Protein Kinase 1Mitogen-Activated Protein Kinase 3Mitogen-Activated Protein KinasesNF-kappa BPhosphorylationReceptors, InterleukinReceptors, Interleukin-11Signal TransductionSTAT1 Transcription FactorSTAT3 Transcription FactorT-Lymphocytes, CytotoxicTrans-ActivatorsTyrosineUmbilical VeinsConceptsIL-11Mitogen-activated protein kinaseP44 mitogen-activated protein kinaseImmune-Mediated InjuryCytolytic T lymphocytesNF-kappaB activationGp130-signaling cytokinesInflammatory injuryHuman endothelial cellsIL-11 receptorProinflammatory responseMolecule expressionT lymphocytesICAM-1Maximal responseE-selectinMHC AbsVivo modelNF-kappaBEndothelial cellsTyrosine phosphorylationPhospho-STAT3Cultured HUVECsInjuryKinase 1 inhibitor
1999
Apoptosis-inducing Agents Cause Rapid Shedding of Tumor Necrosis Factor Receptor 1 (TNFR1) A NONPHARMACOLOGICAL EXPLANATION FOR INHIBITION OF TNF-MEDIATED ACTIVATION*
Madge L, Sierra-Honigmann M, Pober J. Apoptosis-inducing Agents Cause Rapid Shedding of Tumor Necrosis Factor Receptor 1 (TNFR1) A NONPHARMACOLOGICAL EXPLANATION FOR INHIBITION OF TNF-MEDIATED ACTIVATION*. Journal Of Biological Chemistry 1999, 274: 13643-13649. PMID: 10224136, DOI: 10.1074/jbc.274.19.13643.Peer-Reviewed Original ResearchMeSH KeywordsApoptosisCalcium-Calmodulin-Dependent Protein KinasesCaspasesCells, CulturedDNA-Binding ProteinsEndothelium, VascularEnzyme ActivationEnzyme InhibitorsHumansI-kappa B ProteinsInterleukin-1Mitogen-Activated Protein KinasesNF-KappaB Inhibitor alphaP38 Mitogen-Activated Protein KinasesProteinsReceptors, Tumor Necrosis FactorSignal TransductionTNF Receptor-Associated Factor 1Tumor Necrosis Factor-alphaConceptsTumor necrosis factor receptor 1Apoptogenic drugsIkappaBalpha degradationTNF-dependent recruitmentBroad spectrum caspase inhibitor zVADfmkInitiation of apoptosisCaspase inhibitor zVADfmkApoptotic cell deathApoptosis-inducing agentsEndothelial cellsTumour necrosis factor signalFactor signalsP38 kinaseTNF signalingEvidence of apoptosisCell deathFactor receptor 1Necrosis factor receptor 1Inhibition of TNFArachidonyl trifluoromethylketoneVascular endothelial cellsApoptosisTRADDEC apoptosisPutative inhibitors
1998
Interferon Induction of TAP1
Min W, Pober J, Johnson D. Interferon Induction of TAP1. Circulation Research 1998, 83: 815-823. PMID: 9776728, DOI: 10.1161/01.res.83.8.815.Peer-Reviewed Original ResearchMeSH KeywordsATP Binding Cassette Transporter, Subfamily B, Member 2ATP-Binding Cassette TransportersDimerizationDNA-Binding ProteinsEndotheliumGene Expression Regulation, EnzymologicGenes, ReporterHeLa CellsHumansInterferon-alphaInterferon-betaInterferon-gammaInterferon-Stimulated Gene Factor 3Interferon-Stimulated Gene Factor 3, gamma SubunitIntracellular Signaling Peptides and ProteinsJanus Kinase 1Janus Kinase 2Major Histocompatibility ComplexPromoter Regions, GeneticProtein Tyrosine Phosphatase, Non-Receptor Type 11Protein Tyrosine Phosphatase, Non-Receptor Type 6Protein Tyrosine PhosphatasesProtein-Tyrosine KinasesProto-Oncogene ProteinsRNA, MessengerSH2 Domain-Containing Protein Tyrosine PhosphatasesSignal TransductionSrc Homology DomainsSTAT1 Transcription FactorSTAT2 Transcription FactorTrans-ActivatorsTranscription Factors
1996
A Sustained Reduction in IκB-β May Contribute to Persistent NF-κB Activation in Human Endothelial Cells*
Johnson D, Douglas I, Jahnke A, Ghosh S, Pober J. A Sustained Reduction in IκB-β May Contribute to Persistent NF-κB Activation in Human Endothelial Cells*. Journal Of Biological Chemistry 1996, 271: 16317-16322. PMID: 8663191, DOI: 10.1074/jbc.271.27.16317.Peer-Reviewed Original ResearchMeSH KeywordsBase SequenceBinding SitesCell Adhesion MoleculesCell MembraneCell NucleusCells, CulturedConsensus SequenceCytosolDNADNA-Binding ProteinsEndothelium, VascularGene ExpressionHistocompatibility Antigens Class IHumansI-kappa B ProteinsIntercellular Adhesion Molecule-1Interleukin-1KineticsMolecular Sequence DataNF-kappa BOligodeoxyribonucleotidesTetradecanoylphorbol AcetateTumor Necrosis Factor-alphaUmbilical VeinsVascular Cell Adhesion Molecule-1ConceptsAdhesion molecule-1Vascular cell adhesion molecule-1Intercellular adhesion molecule-1Cell adhesion molecule-1Phorbol myristate acetateInterleukin-1alphaNF-kappaB activationMolecule-1Sustained reductionNF-kappaBEndothelial cellsIkappaB-alphaIkappaB-betaHuman endothelial cellsHuman leukocyte antigen (HLA) class INF-kappaB.Inhibitory protein IkappaB-alphaCell surface molecule expressionIkappaB-beta degradationPersistent NF-κB activationSurface molecule expressionAntigen class INF-κB activationIkappaB-beta levelsDe novo expressionKinetically coordinated induction of TAP1 and HLA class I by IFN-gamma: the rapid induction of TAP1 by IFN-gamma is mediated by Stat1 alpha.
Min W, Pober JS, Johnson DR. Kinetically coordinated induction of TAP1 and HLA class I by IFN-gamma: the rapid induction of TAP1 by IFN-gamma is mediated by Stat1 alpha. The Journal Of Immunology 1996, 156: 3174-83. PMID: 8617938, DOI: 10.4049/jimmunol.156.9.3174.Peer-Reviewed Original ResearchAllelesATP Binding Cassette Transporter, Subfamily B, Member 2ATP-Binding Cassette TransportersBase SequenceCysteine EndopeptidasesDNA-Binding ProteinsEndopeptidasesExtracellular Matrix ProteinsGene Expression RegulationHeLa CellsHistocompatibility Antigens Class IHLA AntigensHLA-B AntigensHumansInterferon Regulatory Factor-1Interferon-gammaInterferon-Stimulated Gene Factor 3KineticsMacromolecular SubstancesMolecular Sequence DataNerve Tissue ProteinsNF-kappa BPhosphoproteinsPromoter Regions, GeneticRNA, MessengerTranscription FactorsTranscription, Genetic
1994
cAMP and tumor necrosis factor competitively regulate transcriptional activation through and nuclear factor binding to the cAMP-responsive element/activating transcription factor element of the endothelial leukocyte adhesion molecule-1 (E-selectin) promoter.
De Luca LG, Johnson DR, Whitley MZ, Collins T, Pober JS. cAMP and tumor necrosis factor competitively regulate transcriptional activation through and nuclear factor binding to the cAMP-responsive element/activating transcription factor element of the endothelial leukocyte adhesion molecule-1 (E-selectin) promoter. Journal Of Biological Chemistry 1994, 269: 19193-19196. PMID: 7518452, DOI: 10.1016/s0021-9258(17)32150-6.Peer-Reviewed Original ResearchConceptsConsensus cAMP-responsive elementCRE-binding proteinTranscription factor elementsTranscriptional activationCRE/ATF elementElectrophoretic mobility shift assaysMobility shift assaysTransient transfection assaysAntibody supershift assaysCAMP-responsive elementMigrating formPromoter elementsDNA sequencesFastest migrating formBovine aortic endothelial cellsShift assaysTransfection assaysPromoter responseSupershift assaysGene expressionFactor elementsC-JunAortic endothelial cellsEffects of TNFProteinHLA class I heavy-chain gene promoter elements mediating synergy between tumor necrosis factor and interferons.
Johnson DR, Pober JS. HLA class I heavy-chain gene promoter elements mediating synergy between tumor necrosis factor and interferons. Molecular And Cellular Biology 1994, 14: 1322-1332. PMID: 8289810, PMCID: PMC358487, DOI: 10.1128/mcb.14.2.1322.Peer-Reviewed Original ResearchBase SequenceBinding SitesCloning, MolecularConsensus SequenceDNA PrimersDNA-Binding ProteinsDrug SynergismGene ExpressionGenes, MHC Class IHeLa CellsHLA-B7 AntigenHumansInterferon-betaInterferon-gammaLymphotoxin-alphaMolecular Sequence DataNF-kappa BNuclear ProteinsPolymerase Chain ReactionPromoter Regions, GeneticRecombinant ProteinsSequence DeletionSequence Homology, Nucleic AcidTransfection