2013
Alloantibody and Complement Promote T Cell–Mediated Cardiac Allograft Vasculopathy Through Noncanonical Nuclear Factor-&kgr;B Signaling in Endothelial Cells
Jane-wit D, Manes TD, Yi T, Qin L, Clark P, Kirkiles-Smith NC, Abrahimi P, Devalliere J, Moeckel G, Kulkarni S, Tellides G, Pober JS. Alloantibody and Complement Promote T Cell–Mediated Cardiac Allograft Vasculopathy Through Noncanonical Nuclear Factor-&kgr;B Signaling in Endothelial Cells. Circulation 2013, 128: 2504-2516. PMID: 24045046, PMCID: PMC3885874, DOI: 10.1161/circulationaha.113.002972.Peer-Reviewed Original ResearchConceptsCardiac allograft vasculopathyPanel reactive antibodyNuclear factor-κB signalingFactor-κB signalingAllograft vasculopathyT cellsEndothelial cellsMembrane attack complexAlloreactive T cell activationChronic antibody-mediated rejectionNoncanonical nuclear factorProinflammatory gene programAntibody-mediated rejectionDonor-specific antibodiesGraft endothelial cellsLate allograft lossAlloreactive T cellsAllogeneic endothelial cellsT cell activationAttack complexHuman T cellsAllograft lossHeart transplantationTransplantation patientsLesion pathogenesis
2011
Transforming Growth Factor Beta Expression by Human Vascular Cells Inhibits Interferon Gamma Production and Arterial Media Injury by Alloreactive Memory T Cells
Lebastchi AH, Khan SF, Qin L, Li W, Zhou J, Hibino N, Yi T, Rao DA, Pober JS, Tellides G. Transforming Growth Factor Beta Expression by Human Vascular Cells Inhibits Interferon Gamma Production and Arterial Media Injury by Alloreactive Memory T Cells. American Journal Of Transplantation 2011, 11: 2332-2341. PMID: 21812925, PMCID: PMC3203343, DOI: 10.1111/j.1600-6143.2011.03676.x.Peer-Reviewed Original ResearchConceptsEffector T cellsMemory T cellsNaïve T cellsT cellsIL-17Vascular cellsEffector memory T cellsAlloreactive memory T cellsGrowth factor-beta expressionMedial smooth muscle cellsHuman effector memory T cellsHuman coronary artery graftsIL-17 productionCoronary artery graftsIFN-γ productionAllogeneic endothelial cellsInterferon-gamma productionDevelopment of arteriosclerosisExpression of TGFRole of TGFSmooth muscle cellsImmunodeficient mouse hostsHuman T cellsIntimal compartmentMedial infiltration
2001
Human Vascular Endothelial Cells Stimulate a Lower Frequency of Alloreactive CD8+ Pre-CTL and Induce Less Clonal Expansion than Matching B Lymphoblastoid Cells: Development of a Novel Limiting Dilution Analysis Method Based on CFSE Labeling of Lymphocytes
Dengler T, Johnson D, Pober J. Human Vascular Endothelial Cells Stimulate a Lower Frequency of Alloreactive CD8+ Pre-CTL and Induce Less Clonal Expansion than Matching B Lymphoblastoid Cells: Development of a Novel Limiting Dilution Analysis Method Based on CFSE Labeling of Lymphocytes. The Journal Of Immunology 2001, 166: 3846-3854. PMID: 11238628, DOI: 10.4049/jimmunol.166.6.3846.Peer-Reviewed Original ResearchAntigens, CDB-Lymphocyte SubsetsCD8-Positive T-LymphocytesCell DivisionCell Line, TransformedCells, CulturedClone CellsCoculture TechniquesColony-Forming Units AssayCytotoxicity Tests, ImmunologicEndothelium, VascularFlow CytometryFluoresceinsFluorescent DyesHumansImmunologic MemoryInterphaseIsoantigensLymphocyte ActivationLymphocyte CountStem CellsSuccinimidesT-Lymphocyte SubsetsT-Lymphocytes, Regulatory
2000
Human Vascular Endothelial Cells Stimulate Memory But Not Naive CD8+ T Cells to Differentiate into CTL Retaining an Early Activation Phenotype
Dengler T, Pober J. Human Vascular Endothelial Cells Stimulate Memory But Not Naive CD8+ T Cells to Differentiate into CTL Retaining an Early Activation Phenotype. The Journal Of Immunology 2000, 164: 5146-5155. PMID: 10799873, DOI: 10.4049/jimmunol.164.10.5146.Peer-Reviewed Original ResearchMeSH KeywordsB-LymphocytesCD8-Positive T-LymphocytesCell DifferentiationCells, CulturedCoculture TechniquesCyclosporineCytotoxicity Tests, ImmunologicEndothelium, VascularHistocompatibility Antigens Class IHumansImmunologic MemoryImmunophenotypingInterleukin-12InterphaseLeukocyte Common AntigensLymphocyte ActivationT-Lymphocyte SubsetsT-Lymphocytes, CytotoxicConceptsB lymphoblastoid cellsT cellsNaive CD8Endothelial cellsImmunoregulatory cell typesIntracellular perforin contentAlloreactive T cellsAnti-CD28 mAbHuman vascular endothelial cellsHigh surface expressionVascular endothelial cellsExpansion of memoryConventional CTLGraft parenchymaGraft rejectionMemory CD8CTL generationPerforin contentCTL expansionVascular injuryHuman CD8CD40 ligandAlloreactive CTLAnatomic compartmentsICAM-1
1998
Human endothelial cells effectively costimulate cytokine production by, but not differentiation of, naive CD4+ T cells.
Ma W, Pober J. Human endothelial cells effectively costimulate cytokine production by, but not differentiation of, naive CD4+ T cells. The Journal Of Immunology 1998, 161: 2158-67. PMID: 9725207, DOI: 10.4049/jimmunol.161.5.2158.Peer-Reviewed Original ResearchMeSH KeywordsAdjuvants, ImmunologicAdultCD4-Positive T-LymphocytesCD58 AntigensCell AdhesionCell DifferentiationCells, CulturedCytokinesEndothelium, VascularHumansImmunologic MemoryInterferon-gammaInterleukin-12Interleukin-2Interleukin-4InterphaseLeukocytes, MononuclearLymphocyte ActivationT-Lymphocyte SubsetsTh1 CellsUmbilical VeinsConceptsPeripheral blood adherent mononuclear cellsExogenous IL-12Naive T cellsT cellsIL-12Endothelial cellsMemory CD4Human endothelial cellsCytokine productionNaive CD4B7 moleculesIL-2IFN-gammaBone marrow-derived APCsBlood adherent mononuclear cellsB7 molecule expressionIL-12 supplementationIntracellular cytokine stainingTh1-like cellsIL-4 productionIFN-gamma productionCytokine stainingMononuclear cellsIL-4Molecule expression
1996
CAN GRAFT ENDOTHELIAL CELLS INITIATE A HOST ANTI-GRAFT IMMUNE RESPONSE?1
Pober2 3, G. O, L. R, O.S. S. CAN GRAFT ENDOTHELIAL CELLS INITIATE A HOST ANTI-GRAFT IMMUNE RESPONSE?1. Transplantation 1996, 61: 343-349. PMID: 8610337, DOI: 10.1097/00007890-199602150-00001.Commentaries, Editorials and Letters
1994
Endothelial cell-induced resistance to cyclosporin A in human peripheral blood T cells requires contact-dependent interactions involving CD2 but not CD28.
Karmann K, Pober JS, Hughes CC. Endothelial cell-induced resistance to cyclosporin A in human peripheral blood T cells requires contact-dependent interactions involving CD2 but not CD28. The Journal Of Immunology 1994, 153: 3929-37. PMID: 7523510, DOI: 10.4049/jimmunol.153.9.3929.Peer-Reviewed Original ResearchConceptsIL-2 secretionT cellsEndothelial cellsPeripheral blood T cellsHuman peripheral blood T cellsGraft endothelial cellsHost T cellsBlood T cellsT cell CD28Cultured human endothelial cellsEndothelial cell ligandsIL-2 synthesisInitiation of cocultureContact-dependent interactionsEffect of ECHuman endothelial cellsT cell CD2Presence of ECInhibitory doseLFA-3Cell ligandsCyclosporin ACD28Cell contactSecretion
1993
HUMAN CD4+ T CELLS PROLIFERATE TO HLA-DR+ ALLOGENEIC VASCULAR ENDOTHELIUM
SAVAGE C, HUGHES C, MCINTYRE B, PICARD J, POBER J. HUMAN CD4+ T CELLS PROLIFERATE TO HLA-DR+ ALLOGENEIC VASCULAR ENDOTHELIUM. Transplantation 1993, 56: 128-134. PMID: 7687392, DOI: 10.1097/00007890-199307000-00024.Peer-Reviewed Original ResearchMeSH KeywordsAntibodies, MonoclonalAntigens, CDAntigens, Differentiation, T-LymphocyteCD2 AntigensCD4 AntigensCD58 AntigensCells, CulturedEndothelium, VascularFlow CytometryHistocompatibility Antigens Class IHLA-DR AntigensHumansInterferon-gammaLymphocyte ActivationMembrane GlycoproteinsPolymerase Chain ReactionReceptors, ImmunologicRecombinant ProteinsT-Lymphocyte SubsetsTransplantation, HomologousUmbilical VeinsConceptsHLA-DR expressing cellsT cellsHLA-DRPolymerase chain reactionEC culturesAllogeneic class II MHC moleculesMHC moleculesLFA-1 beta chainLFA-1Peripheral blood CD4VLA-4 alphaMHC class II moleculesT cell responsesClass II MHC moleculesT cell surface moleculesT cell proliferationClass I MHC moleculesExpressing cellsClass II moleculesBeta chainII MHC moleculesDe novo expressionI MHC moleculesAllogeneic proliferationBlood CD4
1992
Expression of vascular cell adhesion molecule-1 in human renal allografts.
Briscoe DM, Pober JS, Harmon WE, Cotran RS. Expression of vascular cell adhesion molecule-1 in human renal allografts. Journal Of The American Society Of Nephrology 1992, 3: 1180-5. PMID: 1282831, DOI: 10.1681/asn.v351180.Peer-Reviewed Original ResearchMeSH KeywordsAcute DiseaseAntigens, CDAntigens, Differentiation, MyelomonocyticCD3 ComplexCell Adhesion MoleculesE-SelectinEndothelium, VascularGraft RejectionHumansIntercellular Adhesion Molecule-1Kidney TransplantationKidney Tubular Necrosis, AcuteKidney TubulesMacrophagesMonocytesPostoperative ComplicationsT-Lymphocyte SubsetsTransplantation, HomologousVascular Cell Adhesion Molecule-1ConceptsVascular cell adhesion molecule-1Cell adhesion molecule-1VCAM-1 expressionAdhesion molecule-1Renal allograftsNormal biopsiesMolecule-1Human renal allograft biopsiesMonocyte/macrophage infiltrateEndothelial VCAM-1 expressionAcute tubular necrosisRenal allograft biopsiesT-cell infiltratesT cell infiltrationHuman renal allograftsEndothelial VCAM-1Normal kidney specimensArea of infiltratesMean scoreVascular endothelial cellsAcute rejectionAllograft biopsiesTubular necrosisCell infiltrateClinicopathologic diagnosis
1991
Endothelial cell lymphocyte function-associated antigen-3 and an unidentified ligand act in concert to provide costimulation to human peripheral blood CD4+ T cells
Savage C, Hughes C, Pepinsky R, Wallner B, Freedman A, Pober J. Endothelial cell lymphocyte function-associated antigen-3 and an unidentified ligand act in concert to provide costimulation to human peripheral blood CD4+ T cells. Cellular Immunology 1991, 137: 150-163. PMID: 1679377, DOI: 10.1016/0008-8749(91)90065-j.Peer-Reviewed Original ResearchConceptsLymphocyte function-associated antigen-3Adhesion molecule-1T cellsEndothelial cellsMolecule-1Antigen 3Human peripheral blood CD4Vascular cell adhesion molecule-1Intercellular adhesion molecule-1Cell adhesion molecule-1Peripheral blood CD4T cell populationsB7/BB1Cultured human endothelial cellsIL-2 synthesisBlood CD4EC costimulationHuman endothelial cellsT cell CD2IL-2CD4CostimulationCell populationsComparable degreeLines of evidenceINDUCED EXPRESSION OF ENDOTHELIAL-LEUKOCYTE ADHESION MOLECULES IN HUMAN CARDIAC ALLOGRAFTS
Briscoe DM, Schoen FJ, Rice GE, Bevilacqua MP, Ganz P, Pober JS. INDUCED EXPRESSION OF ENDOTHELIAL-LEUKOCYTE ADHESION MOLECULES IN HUMAN CARDIAC ALLOGRAFTS. Transplantation 1991, 51: 537-539. PMID: 1704649, DOI: 10.1097/00007890-199102000-00050.Peer-Reviewed Original Research