2023
Metabolic reprogramming by immune-responsive gene 1 up-regulation improves donor heart preservation and function
Lei I, Huang W, Noly P, Naik S, Ghali M, Liu L, Pagani F, Abou El Ela A, Pober J, Pitt B, Platt J, Cascalho M, Wang Z, Chen Y, Mortensen R, Tang P. Metabolic reprogramming by immune-responsive gene 1 up-regulation improves donor heart preservation and function. Science Translational Medicine 2023, 15: eade3782. PMID: 36753565, PMCID: PMC10068866, DOI: 10.1126/scitranslmed.ade3782.Peer-Reviewed Original ResearchConceptsImmune response gene 1Primary graft dysfunctionDonor heart preservationValproic acidDonor heartsVPA treatmentHeart preservationHeart functionImmune-responsive gene 1Donor heart functionHistone deacetylase inhibitor valproic acidImproved heart functionAntioxidant protein expressionMetabolic reprogrammingDonor-recipient matchingPromising therapeutic strategyHuman donor heartsInhibitor valproic acidGene 1Graft dysfunctionCardioprotective effectsKetoglutarate solutionTherapeutic strategiesResponse gene-1Nuclear factor
2001
HUMAN T CELLS INFILTRATE AND INJURE PIG CORONARY ARTERY GRAFTS WITH ACTIVATED BUT NOT QUIESCENT ENDOTHELIUM IN IMMUNODEFICIENT MOUSE HOSTS1
Tereb D, Kirkiles-Smith N, Kim R, Wang Y, Rudic R, Schechner J, Lorber M, Bothwell A, Pober J, Tellides G. HUMAN T CELLS INFILTRATE AND INJURE PIG CORONARY ARTERY GRAFTS WITH ACTIVATED BUT NOT QUIESCENT ENDOTHELIUM IN IMMUNODEFICIENT MOUSE HOSTS1. Transplantation 2001, 71: 1622-1630. PMID: 11435975, DOI: 10.1097/00007890-200106150-00023.Peer-Reviewed Original ResearchConceptsTumor necrosis factorHuman tumor necrosis factorArtery graftT cellsNecrosis factorPig coronary artery endothelial cellsHuman T-cell infiltrationVascular cell adhesion molecule-1Human peripheral blood mononuclearHuman peripheral blood mononuclear cellsPeripheral blood mononuclear cellsHuman leukocytesEndothelial cellsMajor histocompatibility complex antigensCell adhesion molecule-1Graft endothelial cellsT-cell infiltratesBeige mouse modelCoronary artery endothelial cellsT cell infiltrationCoronary artery graftsBlood mononuclear cellsPeripheral blood mononuclearCoronary artery segmentsAdhesion molecule-1Endothelial cell activation by tumor necrosis factor elicits human antiporcine cell-mediated rejection responses
Kirkiles-Smith NC, Tereb DA, Kim RW, McNiff JM, Schechner JS, Lorber MI, Pober JS, Tellides G. Endothelial cell activation by tumor necrosis factor elicits human antiporcine cell-mediated rejection responses. Transplantation Proceedings 2001, 33: 412-413. PMID: 11266888, DOI: 10.1016/s0041-1345(00)02072-8.Peer-Reviewed Original ResearchMeSH KeywordsAdultAnimalsCytokinesEndothelium, VascularGraft RejectionHistocompatibility Antigens Class IHistocompatibility Antigens Class IIHumansLymphocyte ActivationMiceMice, SCIDModels, AnimalSkin TransplantationSpecies SpecificitySwineT-LymphocytesTransplantation ChimeraTransplantation ToleranceTransplantation, HeterologousTumor Necrosis Factor-alpha
2000
Human TNF Can Induce Nonspecific Inflammatory and Human Immune-Mediated Microvascular Injury of Pig Skin Xenografts in Immunodeficient Mouse Hosts
Kirkiles-Smith N, Tereb D, Kim R, McNiff J, Schechner J, Lorber M, Pober J, Tellides G. Human TNF Can Induce Nonspecific Inflammatory and Human Immune-Mediated Microvascular Injury of Pig Skin Xenografts in Immunodeficient Mouse Hosts. The Journal Of Immunology 2000, 164: 6601-6609. PMID: 10843720, DOI: 10.4049/jimmunol.164.12.6601.Peer-Reviewed Original ResearchMeSH KeywordsAdoptive TransferAdultAnimalsCell Adhesion MoleculesDose-Response Relationship, ImmunologicDrug SynergismEndothelium, VascularGraft RejectionHistocompatibility AntigensHumansInterferon-gammaMiceMice, Inbred C57BLMice, SCIDMicrocirculationSevere Combined ImmunodeficiencySkin TransplantationSwineT-LymphocytesTransplantation, HeterologousTumor Necrosis Factor-alphaUp-RegulationConceptsHuman T-cell infiltrationPig skin xenograftsT cell infiltrationSkin xenograftsCell infiltrationHuman TNFIFN-gammaE-selectinT cell-mediated rejectionEndothelial cellsAdhesion moleculesCytokine-mediated injuryNonspecific inflammatory damagePig skin graftsCell-mediated rejectionAppropriate therapeutic targetsImmunodeficient mouse modelPorcine E-selectinEndothelial adhesion moleculesPorcine IFN-gammaClass II moleculesHuman skin allograftsMHC class IPorcine endothelial cellsImmunodeficient mouse hostsInterferon-γ elicits arteriosclerosis in the absence of leukocytes
Tellides G, Tereb D, Kirkiles-Smith N, Kim R, Wilson J, Schechner J, Lorber M, Pober J. Interferon-γ elicits arteriosclerosis in the absence of leukocytes. Nature 2000, 403: 207-211. PMID: 10646607, DOI: 10.1038/35003221.Peer-Reviewed Original ResearchMeSH KeywordsAdultAnimalsArteriosclerosisCell DivisionCells, CulturedCoronary VesselsHistocompatibility AntigensHumansImage Processing, Computer-AssistedImmunohistochemistryInterferon-gammaLeukocytesMiceMice, SCIDMuscle, Smooth, VascularPlatelet-Derived Growth FactorReceptor, Platelet-Derived Growth Factor betaSwineTransplantation, HeterologousConceptsVascular smooth muscle cellsGraft arteriosclerosisIntimal expansionAbsence of leukocytesLesions of atherosclerosisSmooth muscle cellsAllogeneic transplantationArteriosclerotic changesAtheroma formationCytokine interferonExogenous IFNAntigen presentationT cellsImmunodeficient miceMononuclear leukocytesMouse modelArterial intimaIFNMuscle cellsArteriosclerosisLeukocytesHuman arteriesAtherosclerosisCellsTransplantation
1998
Allogeneic and xenogeneic vascular injury and protection
Pober JS, Schechner JS, Murray AG, Sultan P, Kirkiles N, Tereb D, Wilson J, McNiff JM, Askenase PW, Tellides G, Lorber MI. Allogeneic and xenogeneic vascular injury and protection. Transplantation Proceedings 1998, 30: 4168-4169. PMID: 9865336, DOI: 10.1016/s0041-1345(98)01381-5.Peer-Reviewed Original Research
1997
Pig but not human interferon-γ initiates human cell-mediated rejection of pig tissue in vivo
Sultan P, Murray A, McNiff J, Lorber M, Askenase P, Bothwell A, Pober J. Pig but not human interferon-γ initiates human cell-mediated rejection of pig tissue in vivo. Proceedings Of The National Academy Of Sciences Of The United States Of America 1997, 94: 8767-8772. PMID: 9238052, PMCID: PMC23121, DOI: 10.1073/pnas.94.16.8767.Peer-Reviewed Original ResearchConceptsHuman peripheral blood mononuclear cellsPeripheral blood mononuclear cellsPig skin graftsCell-mediated rejectionBlood mononuclear cellsIFN-gammaMononuclear cellsSkin graftsDermal microvesselsMajor histocompatibility complex (MHC) molecule expressionT cell-mediated rejectionPerivascular mononuclear cell infiltratesClass II major histocompatibility complex moleculesMononuclear cell infiltrateMajor histocompatibility complex moleculesTumor necrosis factorEvidence of injuryHistocompatibility complex moleculesPig endothelial cellsCell infiltrateIntradermal injectionMolecule expressionSparse infiltrateIntraperitoneal inoculationMouse microvessels
1996
Porcine endothelial CD86 is a major costimulator of xenogeneic human T cells: cloning, sequencing, and functional expression in human endothelial cells.
Maher SE, Karmann K, Min W, Hughes CC, Pober JS, Bothwell AL. Porcine endothelial CD86 is a major costimulator of xenogeneic human T cells: cloning, sequencing, and functional expression in human endothelial cells. The Journal Of Immunology 1996, 157: 3838-44. PMID: 8892613, DOI: 10.4049/jimmunol.157.9.3838.Peer-Reviewed Original ResearchAbataceptAmino Acid SequenceAnimalsAntigens, CDAntigens, DifferentiationAortaB7-2 AntigenBase SequenceCells, CulturedCHO CellsCloning, MolecularCricetinaeCricetulusCTLA-4 AntigenDNA, ComplementaryEndothelium, VascularHumansImmunoconjugatesInterleukin-2Lymphocyte ActivationMembrane GlycoproteinsMolecular Sequence DataSequence AlignmentSequence Homology, Amino AcidSpecies SpecificitySwineT-LymphocytesTransfectionUmbilical Veins
1994
Porcine aortic endothelial cells activate human T cells: Direct presentation of MHC antigens and costimulation by ligands for human CD2 and CD28
Murray A, Khodedoust M, Pober J, Bothwell A. Porcine aortic endothelial cells activate human T cells: Direct presentation of MHC antigens and costimulation by ligands for human CD2 and CD28. Immunity 1994, 1: 57-63. PMID: 7889399, DOI: 10.1016/1074-7613(94)90009-4.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntigen PresentationAntigens, HeterophileCD2 AntigensCD28 AntigensCD4-Positive T-LymphocytesCD8-Positive T-LymphocytesCells, CulturedEndothelium, VascularHistocompatibility Antigens Class IHistocompatibility Antigens Class IIHumansIn Vitro TechniquesLigandsLymphocyte ActivationSpecies SpecificitySwineT-LymphocytesConceptsPorcine aortic endothelial cellsHuman umbilical vein endothelial cellsHuman T cellsCTLA-4-IgT cellsAortic endothelial cellsEndothelial cellsCultured porcine aortic endothelial cellsMHC class II moleculesClass II moleculesMHC class IUmbilical vein endothelial cellsVein endothelial cellsAllogeneic responseMHC antigensHuman CD8CD28 pathwayCD8 moleculesHuman CD4Species-specific monoclonal antibodiesCD2 pathwayMonoclonal antibodiesClass IDirect presentationCD4