1998
Interferon Induction of TAP1
Min W, Pober J, Johnson D. Interferon Induction of TAP1. Circulation Research 1998, 83: 815-823. PMID: 9776728, DOI: 10.1161/01.res.83.8.815.Peer-Reviewed Original ResearchMeSH KeywordsATP Binding Cassette Transporter, Subfamily B, Member 2ATP-Binding Cassette TransportersDimerizationDNA-Binding ProteinsEndotheliumGene Expression Regulation, EnzymologicGenes, ReporterHeLa CellsHumansInterferon-alphaInterferon-betaInterferon-gammaInterferon-Stimulated Gene Factor 3Interferon-Stimulated Gene Factor 3, gamma SubunitIntracellular Signaling Peptides and ProteinsJanus Kinase 1Janus Kinase 2Major Histocompatibility ComplexPromoter Regions, GeneticProtein Tyrosine Phosphatase, Non-Receptor Type 11Protein Tyrosine Phosphatase, Non-Receptor Type 6Protein Tyrosine PhosphatasesProtein-Tyrosine KinasesProto-Oncogene ProteinsRNA, MessengerSH2 Domain-Containing Protein Tyrosine PhosphatasesSignal TransductionSrc Homology DomainsSTAT1 Transcription FactorSTAT2 Transcription FactorTrans-ActivatorsTranscription Factors
1997
TNF initiates E-selectin transcription in human endothelial cells through parallel TRAF-NF-kappa B and TRAF-RAC/CDC42-JNK-c-Jun/ATF2 pathways.
Min W, Pober JS. TNF initiates E-selectin transcription in human endothelial cells through parallel TRAF-NF-kappa B and TRAF-RAC/CDC42-JNK-c-Jun/ATF2 pathways. The Journal Of Immunology 1997, 159: 3508-18. PMID: 9317150, DOI: 10.4049/jimmunol.159.7.3508.Peer-Reviewed Original ResearchMeSH KeywordsActivating Transcription Factor 2Antigens, SurfaceCalcium-Calmodulin-Dependent Protein KinasesCyclic AMP Response Element-Binding ProteinE-SelectinEndothelium, VascularGTP-Binding ProteinsHumansJNK Mitogen-Activated Protein KinasesMitogen-Activated Protein KinasesNF-kappa BPhosphorylationPromoter Regions, GeneticProto-Oncogene Proteins c-junRac GTP-Binding ProteinsReceptors, Tumor Necrosis FactorTranscription FactorsTranscription, GeneticTumor Necrosis Factor Receptor-Associated Peptides and ProteinsTumor Necrosis Factor-alphaUmbilical VeinsConceptsTransient overexpressionHuman endothelial cellsC-JunSelectin transcriptionATF2/c-JunN-terminalATF2 pathwayPromoter-reporter geneKappa B elementCAMP-responsive elementEndothelial cellsDomain phosphorylationEndogenous JNKAdaptor proteinInactive JNKNF-kappa BGene transcriptionGene promoterKappa BJNK activationKinase 1TranscriptionTRAF2 proteinCdc42JNK
1996
Kinetically coordinated induction of TAP1 and HLA class I by IFN-gamma: the rapid induction of TAP1 by IFN-gamma is mediated by Stat1 alpha.
Min W, Pober JS, Johnson DR. Kinetically coordinated induction of TAP1 and HLA class I by IFN-gamma: the rapid induction of TAP1 by IFN-gamma is mediated by Stat1 alpha. The Journal Of Immunology 1996, 156: 3174-83. PMID: 8617938, DOI: 10.4049/jimmunol.156.9.3174.Peer-Reviewed Original ResearchAllelesATP Binding Cassette Transporter, Subfamily B, Member 2ATP-Binding Cassette TransportersBase SequenceCysteine EndopeptidasesDNA-Binding ProteinsEndopeptidasesExtracellular Matrix ProteinsGene Expression RegulationHeLa CellsHistocompatibility Antigens Class IHLA AntigensHLA-B AntigensHumansInterferon Regulatory Factor-1Interferon-gammaInterferon-Stimulated Gene Factor 3KineticsMacromolecular SubstancesMolecular Sequence DataNerve Tissue ProteinsNF-kappa BPhosphoproteinsPromoter Regions, GeneticRNA, MessengerTranscription FactorsTranscription, GeneticTranscriptional Regulation of the Interleukin-2 Gene in Normal Human Peripheral Blood T Cells CONVERGENCE OF COSTIMULATORY SIGNALS AND DIFFERENCES FROM TRANSFORMED T CELLS (∗)
Hughes C, Pober J. Transcriptional Regulation of the Interleukin-2 Gene in Normal Human Peripheral Blood T Cells CONVERGENCE OF COSTIMULATORY SIGNALS AND DIFFERENCES FROM TRANSFORMED T CELLS (∗). Journal Of Biological Chemistry 1996, 271: 5369-5377. PMID: 8621390, DOI: 10.1074/jbc.271.10.5369.Peer-Reviewed Original ResearchMeSH KeywordsB-LymphocytesBase SequenceBinding SitesCD3 ComplexCell Line, TransformedCell NucleusCells, CulturedFlow CytometryGene Expression RegulationHumansInterleukin-2KineticsLuciferasesLymphocyte ActivationMolecular Sequence DataNF-kappa BPromoter Regions, GeneticRecombinant ProteinsRegulatory Sequences, Nucleic AcidSignal TransductionT-LymphocytesTranscription FactorsTranscription, GeneticTransfectionTumor Cells, CulturedConceptsNormal T cellsT cellsCostimulatory signalsDifferent costimulatory signalsT cell receptorActivated T-cells (NFAT) sitesNormal human T cellsHuman T cellsT cell sitesTransformed T cellsCD2 antibodiesNF-kappaB siteAccessory cellsTumor cell linesCell receptorInterleukin-2 geneNuclear factorPrimary activationIL-2 promoterJurkat T cellsProximal AP-1 siteCell linesAntibodiesAP-1 siteTranscriptional regulation
1994
cAMP and tumor necrosis factor competitively regulate transcriptional activation through and nuclear factor binding to the cAMP-responsive element/activating transcription factor element of the endothelial leukocyte adhesion molecule-1 (E-selectin) promoter.
De Luca LG, Johnson DR, Whitley MZ, Collins T, Pober JS. cAMP and tumor necrosis factor competitively regulate transcriptional activation through and nuclear factor binding to the cAMP-responsive element/activating transcription factor element of the endothelial leukocyte adhesion molecule-1 (E-selectin) promoter. Journal Of Biological Chemistry 1994, 269: 19193-19196. PMID: 7518452, DOI: 10.1016/s0021-9258(17)32150-6.Peer-Reviewed Original ResearchConceptsConsensus cAMP-responsive elementCRE-binding proteinTranscription factor elementsTranscriptional activationCRE/ATF elementElectrophoretic mobility shift assaysMobility shift assaysTransient transfection assaysAntibody supershift assaysCAMP-responsive elementMigrating formPromoter elementsDNA sequencesFastest migrating formBovine aortic endothelial cellsShift assaysTransfection assaysPromoter responseSupershift assaysGene expressionFactor elementsC-JunAortic endothelial cellsEffects of TNFProteinHLA class I heavy-chain gene promoter elements mediating synergy between tumor necrosis factor and interferons.
Johnson DR, Pober JS. HLA class I heavy-chain gene promoter elements mediating synergy between tumor necrosis factor and interferons. Molecular And Cellular Biology 1994, 14: 1322-1332. PMID: 8289810, PMCID: PMC358487, DOI: 10.1128/mcb.14.2.1322.Peer-Reviewed Original ResearchBase SequenceBinding SitesCloning, MolecularConsensus SequenceDNA PrimersDNA-Binding ProteinsDrug SynergismGene ExpressionGenes, MHC Class IHeLa CellsHLA-B7 AntigenHumansInterferon-betaInterferon-gammaLymphotoxin-alphaMolecular Sequence DataNF-kappa BNuclear ProteinsPolymerase Chain ReactionPromoter Regions, GeneticRecombinant ProteinsSequence DeletionSequence Homology, Nucleic AcidTransfection
1993
Costimulation of peripheral blood T cell activation by human endothelial cells. Enhanced IL-2 transcription correlates with increased c-fos synthesis and increased Fos content of AP-1.
Hughes CC, Pober JS. Costimulation of peripheral blood T cell activation by human endothelial cells. Enhanced IL-2 transcription correlates with increased c-fos synthesis and increased Fos content of AP-1. The Journal Of Immunology 1993, 150: 3148-60. PMID: 8468462, DOI: 10.4049/jimmunol.150.8.3148.Peer-Reviewed Original ResearchConceptsC-fos mRNAIL-2 transcriptionNuclear factorT cellsTranscription factor AP-1PHA activationPeripheral blood T cell activationAP-1Blood T cell activationFactor AP-1C-fosC-JunIL-2 productionHuman umbilical vein ECT cell activationIL-2 mRNAC-Fos proteinEC costimulationEndothelial cells (EC) actAllograft rejectionHuman endothelial cellsIL-2 regulationCostimulatory signalsCell activationKey molecular mechanisms